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. 1970 Mar;65(3):536–543. doi: 10.1073/pnas.65.3.536

Isolation of Double Lysogens from 3T3 Cells Transformed by Plaque Morphology Mutants of SV40*

D R Dubbs 1, Saul Kit 1
PMCID: PMC282940  PMID: 4315613

Abstract

Simian virus 40 (SV40) rescued from 3T3 lines transformed independently by fuzzy plaque strain, SV40 (mKS-U4), and small-clear plaque strain, SV40 (mKS-U88), resembled the virus used to initiate the transformation. When a mixture of the two viruses was used for transformation, both plaque types could be rescued. Two clonal lines, after fusion with CV-1 cells, yielded both plaque types of infectious centers, and a third clonal line, 3T3(4-88)G-1, yielded fuzzy and small-clear plaque types of infectious centers plus large-clear infectious centers resembling „wild-type” SV40 clone 307L. Two secondary and 14 tertiary clones of 3T3(4-88)G-1 were isolated, all of which yielded fuzzy plaque type and clear infectious centers after fusion with CV-1 cells. When these infectious centers were picked and replated on CV-1 cells, the fuzzy and small-clear plaque types of SV40, as well as a large-clear plaque type resembling parental SV40, were isolated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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