Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Mar;65(3):585–592. doi: 10.1073/pnas.65.3.585

Labeling and Other Effects of Actinomycin D on Human Chromosomes*

Charles P Miles 1,†
PMCID: PMC282947  PMID: 5267140

Abstract

3H-actinomycin D, a guanine-binding agent, labels fixed human chromosomes nonrandomly. Actinomycin D added in G2 inhibits secondary constrictions and breaks chromosomes. There is some tendency for label to be concentrated at the ends of chromosomes and near the centromere. Labeling with 3H-thymidine in the late stage of DNA synthesis shows a different pattern and in general lacks the telomeric concentrations. The sites of actinomycin D-induced breaks do not show good correspondence with the sites of actinomycin D label.

Full text

PDF
587

Images in this article

588Image
on p.588

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong D. Serial cultivation of human leukemic cells. Proc Soc Exp Biol Med. 1966 Jun;122(2):475–481. doi: 10.3181/00379727-122-31166. [DOI] [PubMed] [Google Scholar]
  2. Barski G., Cornefert-Jensen F. Cytogenetic study of Sticker venereal sarcoma in European dogs. J Natl Cancer Inst. 1966 Dec;37(6):787–797. [PubMed] [Google Scholar]
  3. COHEN M. M., SHAW M. W. EFFECTS OF MITOMYCIN C ON HUMAN CHROMOSOMES. J Cell Biol. 1964 Nov;23:386–395. doi: 10.1083/jcb.23.2.386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Camargo E. P., Plaut W. The radioautographic detection of DNA with tritiated actinomycin D. J Cell Biol. 1967 Dec;35(3):713–716. doi: 10.1083/jcb.35.3.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clarkson B., Strife A., De Harven E. Continuous culture of seven new cell lines (SK-L1 to 7) from patients with acute leukemia. Cancer. 1967 Jun;20(6):926–947. doi: 10.1002/1097-0142(196706)20:6<926::aid-cncr2820200603>3.0.co;2-4. [DOI] [PubMed] [Google Scholar]
  6. GERMAN J. THE PATTERN OF DNA SYNTHESIS IN THE CHROMOSOMES OF HUMAN BLOOD CELLS. J Cell Biol. 1964 Jan;20:37–55. doi: 10.1083/jcb.20.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GILBERT C. W., MULDAL S., LAJTHA L. G., ROWLEY J. Time-sequence of human chromosome duplication. Nature. 1962 Sep 1;195:869–873. doi: 10.1038/195869a0. [DOI] [PubMed] [Google Scholar]
  8. Hsu T. C., Arrighi F. E., Klevecz R. R., Brinkley B. R. The nucleoli in mitotic divisions of mammalian cells in vitro. J Cell Biol. 1965 Aug;26(2):539–553. doi: 10.1083/jcb.26.2.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KABACK M. M., SAKSELA E., MELLMAN W. J. THE EFFECT OF 5-BROMODEOXYURIDINE ON HUMAN CHROMOSOMES. Exp Cell Res. 1964 Mar;34:182–186. doi: 10.1016/0014-4827(64)90193-4. [DOI] [PubMed] [Google Scholar]
  10. Miles C. P., O'Neill F. 3H labeling patterns of permanent cell line chromosomes showing pulverization or accentuated secondary constrictions. J Cell Biol. 1969 Feb;40(2):553–561. doi: 10.1083/jcb.40.2.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Miles C. P., O'Neill F., Armstrong D., Clarkson B., Keane J. Chromosome patterns of human leukocyte established cell lines. Cancer Res. 1968 Mar;28(3):481–490. [PubMed] [Google Scholar]
  12. Miles C. P., O'Neill F. Chromosome studies of 8 in vitro lines of Burkitt's lymphoma. Cancer Res. 1967 Feb;27(2):392–402. [PubMed] [Google Scholar]
  13. OHNO S., TRUJILLO J. M., KAPLAN W. D., KINOSITA R. Nucleolus-organisers in the causation of chromosomal anomalies in man. Lancet. 1961 Jul 15;2(7194):123–126. doi: 10.1016/s0140-6736(61)92647-2. [DOI] [PubMed] [Google Scholar]
  14. Ostertag W., Kersten W. The action of proflavin and actinomycin D in causing chromatid breakage in human cells. Exp Cell Res. 1965 Aug;39(1):296–301. doi: 10.1016/0014-4827(65)90033-9. [DOI] [PubMed] [Google Scholar]
  15. SIRLIN J. L., JACOB J. SEQUENTIAL AND REVERSIBLE INHIBITION OF SYNTHESIS OF RIBONUCLEIC ACID IN THE NUCLEOLUS AND CHROMOSOMES: EFFECT OF BENZAMIDE AND SUBSTITUTED BENZIMIDAZOLES ON DIPTERAN SALIVARY GLANDS. Nature. 1964 Nov 7;204:545–547. doi: 10.1038/204545a0. [DOI] [PubMed] [Google Scholar]
  16. Simard R. The binding of actinomycin D-3H to heterochromatin as studied by quantitative high resolution radioautography. J Cell Biol. 1967 Dec;35(3):716–722. doi: 10.1083/jcb.35.3.716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Southam C. M., Burchenal J. H., Clarkson B., Tanzi A., Mackey R., McComb V. Hetero- transplantation of human cell lines from Burkitt's tumors and acute leukemia into newborn rats. Cancer. 1969 Feb;23(2):281–299. doi: 10.1002/1097-0142(196902)23:2<281::aid-cncr2820230205>3.0.co;2-#. [DOI] [PubMed] [Google Scholar]
  18. Waring M. J. Drugs which affect the structure and function of DNA. Nature. 1968 Sep 28;219(5161):1320–1325. doi: 10.1038/2191320a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES