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. 1970 Apr;65(4):1033–1040. doi: 10.1073/pnas.65.4.1033

Stimulated Formation of Adenosine 3′,5′-Cyclic Phosphate in Cerebral Cortex: Synergism between Electrical Activity and Biogenic Amines

Hirotoshi Shimizu 1, Cyrus R Creveling 1, John Daly 1
PMCID: PMC283019  PMID: 4314898

Abstract

When cortical slices are incubated with adenine-14C, adenine nucleotides are labeled in a small and relatively stable pool. The ATP-14C of this pool is readily converted to cAMP-14C during incubations with depolarizing agents, such as K+, ouabain, veratridine, or batrachotoxin. During incubations with these agents, release of acetylcholine and of adenosine into the medium is enhanced. The increase in release of adenosine parallels the enhanced formation of cAMP-14C elicited by depolarizing agents, providing further evidence that adenosine may serve to couple electrical activity in the central nervous system with formation of cAMP. When adenosine or a depolarizing agent are incubated, together with a biogenic amine, such as histamine, serotonin, or norepinephrine, the combined effect on cAMP-14C formation in cortical slices is much more than additive. Extracellular levels of biogenic amines could in this manner modulate cAMP formation and biochemical responses in nervous tissue during electrical activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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