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. 1970 Jul;66(3):693–700. doi: 10.1073/pnas.66.3.693

Role of RNA Synthesis in the Estrogen Induction of a Specific Uterine Protein*

Anthony B DeAngelo 1,†, Jack Gorski 1,‡
PMCID: PMC283106  PMID: 5269235

Abstract

The rate of amino acid incorporation into a specific uterine protein (induced protein band) isolated by gel electrophoresis has been shown to be markedly stimulated within an hour after estrogen administration. Injection of actinomycin D (8 mg/kg) prior to estrogen blocks the synthesis of induced protein. The accumulation of the product of the actinomycin D-sensitive step (induced protein band RNA) is significant 15 minutes after estrogen, and its synthesis would appear to be initiated as soon as the estrogen-receptor complex reaches the nucleus. Blocking protein synthesis with puromycin or cycloheximide did not affect the accumulation of induced protein band RNA, indicating that this is one of the earliest macromolecular synthetic events to occur after estrogen administration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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