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. 1970 Jul;66(3):844–849. doi: 10.1073/pnas.66.3.844

Conversion of Bacterial Aldolase from Vegetative to Spore Form by a Sporulation-Specific Protease*

H L Sadoff 1,, Emel Celikkol 1,, H L Engelbrecht 1,§
PMCID: PMC283127  PMID: 4987627

Abstract

A protease is elaborated by cells of Bacillus cereus during sporulation. This sporulation-specific enzyme, in a limited proteolysis, converts vegetative cell aldolase to spore aldolase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ACKERS G. K. MOLECULAR EXCLUSION AND RESTRICTED DIFFUSION PROCESSES IN MOLECULAR-SIEVE CHROMATOGRAPHY. Biochemistry. 1964 May;3:723–730. doi: 10.1021/bi00893a021. [DOI] [PubMed] [Google Scholar]
  2. BERNLOHR R. W., NOVELLI G. D. BACITRACIN BIOSYNTHESIS AND SPORE FORMATION: THE PHYSIOLOGICAL ROLE OF AN ANTIBIOTIC. Arch Biochem Biophys. 1963 Oct;103:94–104. doi: 10.1016/0003-9861(63)90014-6. [DOI] [PubMed] [Google Scholar]
  3. BERNLOHR R. W. POSTLOGARITHMIC PHASE METABOLISM OF SPORULATING MICROORGANISMS. I. PROTEASE OF BACILLUS LICHENIFORMIS. J Biol Chem. 1964 Feb;239:538–543. [PubMed] [Google Scholar]
  4. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  5. Engelbrecht H. L., Sadoff H. L. Properties of purine nucleoside phosphorylases from spores and vegetative cells of Bacillus cereus and their modification by orthophosphate. J Biol Chem. 1969 Nov 25;244(22):6228–6232. [PubMed] [Google Scholar]
  6. FREESE E., PARK S. W., CASHEL M. THE DEVELOPMENTAL SIGNIFICANCE OF ALANINE DEHYDROGENASE IN BACILLUS SUBTILIS. Proc Natl Acad Sci U S A. 1964 Jun;51:1164–1172. doi: 10.1073/pnas.51.6.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GREEN J. H., SADOFF H. L. COMPARISION OF SOLUBLE REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE OXIDASES FROM CELLS AND SPORES OF CLOSTRIDIUM BOTULINUM. J Bacteriol. 1965 Jun;89:1499–1505. doi: 10.1128/jb.89.6.1499-1505.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gardner R., Kornberg A. Biochemical studies of bacterial sporulation and germination. V. Purine nucleoside phosphorylase of vegetative cells and spores of Bacillus cereus. J Biol Chem. 1967 May 25;242(10):2383–2388. [PubMed] [Google Scholar]
  9. HASHIMOTO T., BLACK S. H., GERHARDT P. Development of fine structure, thermostability, and dipicolinate during sporogenesis in a bacillus. Can J Microbiol. 1960 Apr;6:203–212. doi: 10.1139/m60-022. [DOI] [PubMed] [Google Scholar]
  10. Kerjan P., Szulmajster J. DNA dependent RNA polymerase from vegetative cells and from spores of B.Subtilis: III. Isolation of a stimulating factor. FEBS Lett. 1969 Nov 29;5(4):288–290. doi: 10.1016/0014-5793(69)80370-4. [DOI] [PubMed] [Google Scholar]
  11. Kornberg A., Spudich J. A., Nelson D. L., Deutscher M. P. Origin of proteins in sporulation. Annu Rev Biochem. 1968;37:51–78. doi: 10.1146/annurev.bi.37.070168.000411. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Losick R., Sonenshein A. L. Change in the template specificity of RNA polymerase during sporulation of Bacillus subtilis. Nature. 1969 Oct 4;224(5214):35–37. doi: 10.1038/224035a0. [DOI] [PubMed] [Google Scholar]
  14. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  15. RUTTER W. J. EVOLUTION OF ALDOLASE. Fed Proc. 1964 Nov-Dec;23:1248–1257. [PubMed] [Google Scholar]
  16. Sadoff H. L., Hitchins A. D., Celikkol E. Properties of fructose 1,6-diphosphate aldolases from spores and vegetative cells of Bacillus cereus. J Bacteriol. 1969 Jun;98(3):1208–1218. doi: 10.1128/jb.98.3.1208-1218.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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