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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Jul;66(3):882–889. doi: 10.1073/pnas.66.3.882

Isozyme Patterns and Sexual Morphogenesis in Schizophyllum*

Chiu-Sheng Wang 1,, John R Raper 1,
PMCID: PMC283133  PMID: 4393267

Abstract

Isozymes of several different classes of enzymes in partially purified protein extracts of five strains of Schizophyllum commune, isogenic except for genes controlling sexual morphogenesis, were separated on polyacrylamide gel by disc electrophoresis. After staining, isozyme patterns were compared on the bases of the presence or absence, electrophoretic mobility (Rf values), and relative activities of specific isozymes. Differences in isozyme patterns in 14 enzymes, i.e., NADH-dehydrogenase, NADPH-dehydrogenase, a number of NAD and NADP-dependent dehydrogenases, acid phosphatases, leucine aminopeptidase, and esterases, were correlated with the operation or inactivity of the A-and B-sequences of sexual morphogenesis. In only a single instance, i.e., phenolases, no marked differences could be correlated with sexual morphogenesis.

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Selected References

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  1. Britten R. J., Davidson E. H. Gene regulation for higher cells: a theory. Science. 1969 Jul 25;165(3891):349–357. doi: 10.1126/science.165.3891.349. [DOI] [PubMed] [Google Scholar]
  2. Dennen D. W., Niederpruem D. J. Regulation of glutamate dehydrogenases during morphogenesis of Schizophyllum commune. J Bacteriol. 1967 Mar;93(3):904–913. doi: 10.1128/jb.93.3.904-913.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dorn G. Phosphatase mutants in Aspergillus nidulans. Science. 1965 Nov 26;150(3700):1183–1184. doi: 10.1126/science.150.3700.1183. [DOI] [PubMed] [Google Scholar]
  4. PARAG Y. Mutations in the B incompatibility factor of Schizophyllum commune. Proc Natl Acad Sci U S A. 1962 May 15;48:743–750. doi: 10.1073/pnas.48.5.743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. RAPER J. R., ESSER K. Antigenic differences due to the incompatibility factors in Schizophyllum commune. Z Vererbungsl. 1961;92:439–444. doi: 10.1007/BF00890065. [DOI] [PubMed] [Google Scholar]
  6. Raper C. A., Raper J. R. Mutations modifying sexual morphogenesis in schizophyllum. Genetics. 1966 Nov;54(5):1151–1168. doi: 10.1093/genetics/54.5.1151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Raper J. R., Boyd D. H., Raper C. A. Primary and secondary mutations at the incompatibility loci in Schizophyllum. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1324–1332. doi: 10.1073/pnas.53.6.1324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Wang C. S., Raper J. R. Protein specificity and sexual morphogenesis in Schizophyllum commune. J Bacteriol. 1969 Jul;99(1):291–297. doi: 10.1128/jb.99.1.291-297.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Wessels J. G. Biochemistry of sexual morphogenesis in Schizophyllum commune: effect of mutations affecting the incomptability system on cell-wall metabolism. J Bacteriol. 1969 May;98(2):697–704. doi: 10.1128/jb.98.2.697-704.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wessels J. G., Niederpruem D. J. Role of a cell-wall glucan-degrading enzyme in mating of Schizophyllum commune. J Bacteriol. 1967 Nov;94(5):1594–1602. doi: 10.1128/jb.94.5.1594-1602.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

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