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. 1970 Jul;66(3):952–958. doi: 10.1073/pnas.66.3.952

Immunoglobulin Synthesis and Secretion, IV. Sites of Incorporation of Sugars as Determined by Subcellular Fractionation*

Jonathan W Uhr 1,2,†, Isaac Schenkein 1,2
PMCID: PMC283143  PMID: 5269256

Abstract

P3K cells were uniformly labeled with [14C]leucine and also for 1 hr with [3H]leucine, [3]galactose, or [3H]glucosamine. Subcellular fractionation of labeled cells was performed and the amounts of radioactive immunoglobulin G in each fraction were determined. The results indicate that amino acids are incorporated in the rough endoplasmic reticulum, galactose primarily in the smooth endoplasmic reticulum, and glucosamine at both sites. A small portion of radioactivity was found in the microsomal supernatant regardless of the radioisotope employed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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