Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Oct;67(2):674–681. doi: 10.1073/pnas.67.2.674

Replication and Repair of DNA in Cells of Escherichia coli Treated with Toluene*

Robb E Moses 1,†, Charles C Richardson 1,‡
PMCID: PMC283258  PMID: 4943178

Abstract

DNA synthesis has been studied in Escherichia coli cells made permeable to nucleotides by treatment with toluene. Replicative synthesis, as distinguished from repair synthesis, occurs at a rate comparable to that observed in vivo; it is dependent on the presence of all four deoxyribonucleoside triphosphates, but does not require exogenous DNA; and it is stimulated by ATP. Furthermore, replicative synthesis can be abolished at the restrictive temperature in DNA temperature-sensitive mutants. N-ethylmaleimide completely inhibits this type of synthesis, whereas it does not inhibit repair synthesis. Repair synthesis further differs from replicative synthesis in the following points: it does not require ATP; it persists at the restrictive temperature in DNA temperature-sensitive mutants; it can be induced by endogenous or exogenous nuclease activity; and its demonstration requires a Pol+ strain.

Full text

PDF
681

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson E. H. Growth Requirements of Virus-Resistant Mutants of Escherichia Coli Strain "B". Proc Natl Acad Sci U S A. 1946 May;32(5):120–128. doi: 10.1073/pnas.32.5.120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyle J. M., Paterson M. C., Setlow R. B. Excision-repair properties of an Escherichia coli mutant deficient in DNA polymerase. Nature. 1970 May 23;226(5247):708–710. doi: 10.1038/226708a0. [DOI] [PubMed] [Google Scholar]
  3. Buttin G., Wright M. Enzymatic DNA degradation in E. coli: its relationship to synthetic processes at the chromosome level. Cold Spring Harb Symp Quant Biol. 1968;33:259–269. doi: 10.1101/sqb.1968.033.01.030. [DOI] [PubMed] [Google Scholar]
  4. CAIRNS J. The bacterial chromosome and its manner of replication as seen by autoradiography. J Mol Biol. 1963 Mar;6:208–213. doi: 10.1016/s0022-2836(63)80070-4. [DOI] [PubMed] [Google Scholar]
  5. De Lucia P., Cairns J. Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1164–1166. doi: 10.1038/2241164a0. [DOI] [PubMed] [Google Scholar]
  6. Dürwald H., Hoffmann-Berling H. Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants. J Mol Biol. 1968 Jul 14;34(2):331–346. doi: 10.1016/0022-2836(68)90257-x. [DOI] [PubMed] [Google Scholar]
  7. Englund P. T., Huberman J. A., Jovin T. M., Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. XXX. Binding of triphosphates to deoxyribonucleic acid polymerase. J Biol Chem. 1969 Jun 10;244(11):3038–3044. [PubMed] [Google Scholar]
  8. Frankel F. R., Majumdar C., Weintraub S., Frankel D. M. DNA polymerase and the cell membrane after T4 infection. Cold Spring Harb Symp Quant Biol. 1968;33:495–500. doi: 10.1101/sqb.1968.033.01.057. [DOI] [PubMed] [Google Scholar]
  9. Ganesan A. T. Studies on in vitro replication of Bacillus subtilis DNA. Cold Spring Harb Symp Quant Biol. 1968;33:45–57. doi: 10.1101/sqb.1968.033.01.010. [DOI] [PubMed] [Google Scholar]
  10. Gross J., Gross M. Genetic analysis of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1166–1168. doi: 10.1038/2241166a0. [DOI] [PubMed] [Google Scholar]
  11. Jackson R. W., DeMoss J. A. Effects of toluene on Escherichia coli. J Bacteriol. 1965 Nov;90(5):1420–1425. doi: 10.1128/jb.90.5.1420-1425.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kanner L., Hanawalt P. Repair deficiency in a bacterial mutant defective in DNA polymerase. Biochem Biophys Res Commun. 1970 Apr 8;39(1):149–155. doi: 10.1016/0006-291x(70)90770-9. [DOI] [PubMed] [Google Scholar]
  13. Kelly R. B., Cozzarelli N. R., Deutscher M. P., Lehman I. R., Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break. J Biol Chem. 1970 Jan 10;245(1):39–45. [PubMed] [Google Scholar]
  14. Knippers R., Strätling W. The DNA replicating capacity of isolated E. coli cell wall-membrane complexes. Nature. 1970 May 23;226(5247):713–717. doi: 10.1038/226713a0. [DOI] [PubMed] [Google Scholar]
  15. LEVIN D. H., THANG M. N., GRUNBERG-MANAGO M. SYNTHESIS IN VIVO OF POLYNUCLEOTIDE PHOSPHORYLASE IN ESCHERICHIA COLI. I. EFFECT OF AMINO ACIDS ON POLYNUCLEOTIDE PHOSPHORYLASE ACTIVITY IN A CHLORAMPHENICOL-INHIBITED SYSTEM. Biochim Biophys Acta. 1963 Dec 20;76:558–571. doi: 10.1016/0006-3002(63)90082-9. [DOI] [PubMed] [Google Scholar]
  16. NAGATA T. The molecular synchrony and sequential replication of DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1963 Apr;49:551–559. doi: 10.1073/pnas.49.4.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Oishi M. An ATP-dependent deoxyribonuclease from Escherichia coli with a possible role in genetic recombination. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1292–1299. doi: 10.1073/pnas.64.4.1292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. PETTIJOHN D., HANAWALT P. EVIDENCE FOR REPAIR-REPLICATION OF ULTRAVIOLET DAMAGED DNA IN BACTERIA. J Mol Biol. 1964 Aug;9:395–410. doi: 10.1016/s0022-2836(64)80216-3. [DOI] [PubMed] [Google Scholar]
  19. RICHARDSON C. C., INMAN R. B., KORNBERG A. ENZYMIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. 18. THE REPAIR OF PARTIALLY SINGLE-STRANDED DNA TEMPLATES BY DNA POLYMERASE. J Mol Biol. 1964 Jul;9:46–69. doi: 10.1016/s0022-2836(64)80090-5. [DOI] [PubMed] [Google Scholar]
  20. Richardson C. C. Enzymes in DNA metabolism. Annu Rev Biochem. 1969;38:795–840. doi: 10.1146/annurev.bi.38.070169.004051. [DOI] [PubMed] [Google Scholar]
  21. SCHILDKRAUT C. L., RICHARDSON C. C., KORNBERG A. ENZYMIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XVII. SOME UNUSUAL PHYSICAL PROPERTIES OF THE PRODUCT PRIMED BY NATIVE DNA TEMPLATES. J Mol Biol. 1964 Jul;9:24–45. doi: 10.1016/s0022-2836(64)80089-9. [DOI] [PubMed] [Google Scholar]
  22. Smith D. W., Schaller H. E., Bonhoeffer F. J. DNA synthesis in vitro. Nature. 1970 May 23;226(5247):711–713. doi: 10.1038/226711a0. [DOI] [PubMed] [Google Scholar]
  23. YOSHIKAWA H., SUEOKA N. Sequential replication of Bacillus subtilis chromosome. I. Comparison of marker frequencies in exponential and stationary growth phases. Proc Natl Acad Sci U S A. 1963 Apr;49:559–566. doi: 10.1073/pnas.49.4.559. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES