Abstract
High-resolution Fourier transform nuclear magnetic resonance was used to observe the natural abundance 13C spectrum of a 0.02 M solution of bovine pancreatic ribonuclease A. Peaks assignable to only three carbons were easily observed after 10 hr of signal averaging. A number of tentative assignments were made.
As expected, the 13C spectrum was appreciably richer in detail than the corresponding proton spectrum. Natural abundance 13C Fourier transform nmr appears to be a practical tool for the study of biopolymers in solution.
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Selected References
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