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. 1970 Nov;67(3):1214–1220. doi: 10.1073/pnas.67.3.1214

Behavior of Tissue Culture Cells Infected with Polyoma Virus*

Renato Dulbecco 1
PMCID: PMC283339  PMID: 4320974

Abstract

Infection of tissue culture cells with the oncogenic polyoma virus, or its temperature-sensitive mutant Ts-a, causes several changes other than the previously known induction of cellular DNA synthesis. Cellular movement, survival, and mitotic rate are enhanced in low-serum medium, and morphology is changed; the cellular growth parameters, wound serum requirement, and topoinhibition are markedly decreased. The changes are similar to those that occur in cell transformation and are produced by viral functions known to be expressed in transformed cells. Clues as to the possible mechanisms of all these changes are analyzed and a possible mechanism is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aloni Y., Winocour E., Sachs L. Characterization of the simian virus 40-specific RNA in virus-yielding and transformed cells. J Mol Biol. 1968 Feb 14;31(3):415–429. doi: 10.1016/0022-2836(68)90418-x. [DOI] [PubMed] [Google Scholar]
  2. Branton P. E., Sheinin R. Control of DNA synthesis in cells infected with polyoma virus. Virology. 1968 Dec;36(4):652–661. doi: 10.1016/0042-6822(68)90196-7. [DOI] [PubMed] [Google Scholar]
  3. DULBECCO R. TRANSFORMATION OF CELLS IN VITRO BY DNA-CONTAINING VIRUSES. JAMA. 1964 Nov 23;190:721–726. doi: 10.1001/jama.1964.03070210027005. [DOI] [PubMed] [Google Scholar]
  4. Dulbecco R. Cell transformation by viruses. Science. 1969 Nov 21;166(3908):962–968. doi: 10.1126/science.166.3908.962. [DOI] [PubMed] [Google Scholar]
  5. Dulbecco R., Stoker M. G. Conditions determining initiation of DNA synthesis in 3T3 cells. Proc Natl Acad Sci U S A. 1970 May;66(1):204–210. doi: 10.1073/pnas.66.1.204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fried M. Characterization of a temperature-sensitive mutant of polyoma virus. Virology. 1970 Mar;40(3):605–617. doi: 10.1016/0042-6822(70)90205-9. [DOI] [PubMed] [Google Scholar]
  7. Fried M., Pitts J. D. Replication of polyoma virus DNA. I. A resting cell system for biochemical studies on polyoma virus. Virology. 1968 Apr;34(4):761–770. doi: 10.1016/0042-6822(68)90097-4. [DOI] [PubMed] [Google Scholar]
  8. Hatanaka M., Dulbecco R. Induction of DNA synthesis by SV40. Proc Natl Acad Sci U S A. 1966 Aug;56(2):736–740. doi: 10.1073/pnas.56.2.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  10. Kit S., De Torres R. A., Dubbs D. R., Salvi M. L. Induction of cellular deoxyribonuleic acid synthesis by simian virus 40. J Virol. 1967 Aug;1(4):738–746. doi: 10.1128/jvi.1.4.738-746.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Oda K., Dulbecco R. Regulation of transcription of the SV40 DNA in productively infected and in transformed cells. Proc Natl Acad Sci U S A. 1968 Jun;60(2):525–532. doi: 10.1073/pnas.60.2.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stoker M. Abortive transformation by polyoma virus. Nature. 1968 Apr 20;218(5138):234–238. doi: 10.1038/218234a0. [DOI] [PubMed] [Google Scholar]
  13. TODARO G. J., GREEN H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol. 1963 May;17:299–313. doi: 10.1083/jcb.17.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Todaro G. J., Aaronson S. A. Properties of clonal lines of murine sarcoma virus transformed Balb-3T3 cells. Virology. 1969 May;38(1):174–179. doi: 10.1016/0042-6822(69)90140-8. [DOI] [PubMed] [Google Scholar]
  15. VOGT M., DULBECCO R. Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology. 1962 Jan;16:41–51. doi: 10.1016/0042-6822(62)90200-3. [DOI] [PubMed] [Google Scholar]
  16. Weil R., Michel M. R., Ruschmann G. K. Induction of cellular DNA synthesis by polyoma virus. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1468–1475. doi: 10.1073/pnas.53.6.1468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Winocour E., Kaye A. M., Stollar V. Synthesis and transmethylation of DNA in polyoma-infected cultures. Virology. 1965 Oct;27(2):156–169. doi: 10.1016/0042-6822(65)90155-8. [DOI] [PubMed] [Google Scholar]

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