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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Nov;67(3):1493–1500. doi: 10.1073/pnas.67.3.1493

Development of Insulin-Sensitivity by Mouse Mammary Gland In Vitro*

S H Friedberg 1, Takami Oka 1, Yale J Topper 1
PMCID: PMC283381  PMID: 5274474

Abstract

Explants from mammary gland of pregnant mice are sensitive to insulin, but corresponding explants from mature, virgin mice are insensitive to insulin in certain respects. Isolated virgin tissue can acquire insulin-sensitivity independent of exogenous insulin. Some endocrinological and developmental implications are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Espinosa de los Monteros, Driscoll S. G., Steinke J. Insulin release from isolated human fetal pancreatic islets. Science. 1970 May 29;168(3935):1111–1112. doi: 10.1126/science.168.3935.1111. [DOI] [PubMed] [Google Scholar]
  2. GLOCK G. E., McLEAN P. Levels of enzymes of the direct oxidative pathway of carbohydrate metabolism in mammalian tissues and tumours. Biochem J. 1954 Jan;56(1):171–175. doi: 10.1042/bj0560171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Greengard O., Sahib M. K., Knox W. E. Developmental formation and distribution of arginase in rat tissues. Arch Biochem Biophys. 1970 Apr;137(2):477–482. doi: 10.1016/0003-9861(70)90465-0. [DOI] [PubMed] [Google Scholar]
  4. Juergens W. G., Stockdale F. E., Topper Y. J., Elias J. J. Hormone-dependent differentiation of mammary gland in vitro. Proc Natl Acad Sci U S A. 1965 Aug;54(2):629–634. doi: 10.1073/pnas.54.2.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LASFARGUES E. Y. Cultivation and behavior in vitro of the normal mammary epithelium of the adult mouse. Anat Rec. 1957 Jan;127(1):117–129. doi: 10.1002/ar.1091270111. [DOI] [PubMed] [Google Scholar]
  6. Leader D. P., Barry J. M. Increase in activity of glucose 6-phosphate dehydrogenase in mouse mammary tissue cultured in insulin. Biochem J. 1969 Jun;113(1):175–182. doi: 10.1042/bj1130175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lockwood D. H., Voytovich A. E., Stockdale F. E., Topper Y. J. Insulin-dependent DNA polymerase and DNA synthesis in mammary epithelial cells in vitro. Proc Natl Acad Sci U S A. 1967 Aug;58(2):658–664. doi: 10.1073/pnas.58.2.658. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mills E. S., Topper Y. J. Mammary alveolar epithelial cells: effect of hydrocortisone on ultrastructure. Science. 1969 Sep 12;165(3898):1127–1128. doi: 10.1126/science.165.3898.1127. [DOI] [PubMed] [Google Scholar]
  9. Mills E. S., Topper Y. J. Some ultrastructural effects of insulin, hydrocortisone, and prolactin on mammary gland explants. J Cell Biol. 1970 Feb;44(2):310–328. doi: 10.1083/jcb.44.2.310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Stockdale F. E., Juergens W. G., Topper Y. J. A histological and biochemical study of hormone-dependent differentiation of mammary gland tissue in vitro. Dev Biol. 1966 Apr;13(2):266–281. doi: 10.1016/0012-1606(66)90068-6. [DOI] [PubMed] [Google Scholar]
  11. Stockdale F. E., Topper Y. J. The role of DNA synthesis and mitosis in hormone-dependent differentiation. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1283–1289. doi: 10.1073/pnas.56.4.1283. [DOI] [PMC free article] [PubMed] [Google Scholar]

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