Abstract
A new technique is described for studying controlled in vitro extension of plant cell walls. This technique uses frozen-thawed Avena coleoptile segments in which turgor is replaced by a constant applied force. In this system rapid extension is induced by low pH (3.0-3.6) but not by auxins or CO2. Extensions of over 30% have been achieved. Our results indicate that cell wall synthesis is not required for rapid cell extension, and suggest that wall extension may be controlled by acid-labile, alkali-stable cell wall bonds.
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- Coartney J. S., Morre D. J., Key J. L. Inhibition of RNA synthesis and auxin-induced cell wall extensibility and growth by Actinomycin D. Plant Physiol. 1967 Mar;42(3):434–439. doi: 10.1104/pp.42.3.434. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heyn A. N. Dextranase activity in coleoptiles of Avena. Science. 1970 Feb 6;167(3919):874–875. doi: 10.1126/science.167.3919.874. [DOI] [PubMed] [Google Scholar]
- Lamport D. T. The isolation and partial characterization of hydroxyproline-rich glycopeptides obtained by enzymic degradation of primary cell walls. Biochemistry. 1969 Mar;8(3):1155–1163. doi: 10.1021/bi00831a049. [DOI] [PubMed] [Google Scholar]
- Ray P. M. Radioautographic study of cell wall deposition in growing plant cells. J Cell Biol. 1967 Dec;35(3):659–674. doi: 10.1083/jcb.35.3.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rayle D. L., Cleland R. Enhancement of wall loosening and elongation by Acid solutions. Plant Physiol. 1970 Aug;46(2):250–253. doi: 10.1104/pp.46.2.250. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilson K. The growth of plant cell walls. Int Rev Cytol. 1964;17:1–49. doi: 10.1016/s0074-7696(08)60404-0. [DOI] [PubMed] [Google Scholar]