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. 1992 Oct;66(10):6220–6222. doi: 10.1128/jvi.66.10.6220-6222.1992

Protein P4 of the bacteriophage phi 6 procapsid has a nucleoside triphosphate-binding site with associated nucleoside triphosphate phosphohydrolase activity.

P Gottlieb 1, J Strassman 1, L Mindich 1
PMCID: PMC283676  PMID: 1326667

Abstract

Bacteriophage phi 6 contains three segments of double-stranded RNA. The procapsid consists of proteins P1, P2, P4, and P7, which are encoded by the viral L segment. cDNA copies of this segment have been cloned into plasmids that direct the production of these proteins, which assemble into polyhedral procapsids. These procapsids are capable of packaging plus-sense phi 6 RNA in the presence of nucleoside triphosphate and synthesizing the complementary minus strand to form double-stranded RNA. In this article, we report the presence of a nucleotide-binding site in protein P4. The viral procapsid and nucleocapsid exhibit a nucleoside triphosphate phosphohydrolase activity that converts nucleoside triphosphates into nucleoside diphosphates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoyama A., Hamatake R. K., Hayashi M. Morphogenesis of phi X174: in vitro synthesis of infectious phage from purified viral components. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7285–7289. doi: 10.1073/pnas.78.12.7285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bamford D. H., Mindich L. Electron microscopy of cells infected with nonsense mutants of bacteriophage phi 6. Virology. 1980 Nov;107(1):222–228. doi: 10.1016/0042-6822(80)90287-1. [DOI] [PubMed] [Google Scholar]
  3. Borsa J., Grover J., Chapman J. D. Presence of nucleoside triphosphate phosphohydrolase activity in purified virions of reovirus. J Virol. 1970 Sep;6(3):295–302. doi: 10.1128/jvi.6.3.295-302.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Campbell K. P., MacLennan D. H. Labeling of high affinity ATP binding sites on the 53,000- and 160,000-dalton glycoproteins of the sarcoplasmic reticulum with the photoaffinity probe 8-N3-[alpha-32P]ATP. J Biol Chem. 1983 Feb 10;258(3):1391–1394. [PubMed] [Google Scholar]
  5. Day L. A., Mindich L. The molecular weight of bacteriophage phi 6 and its nucleocapsid. Virology. 1980 Jun;103(2):376–385. doi: 10.1016/0042-6822(80)90196-8. [DOI] [PubMed] [Google Scholar]
  6. Emori Y., Iba H., Okada Y. Transcriptional regulation of three double-stranded RNA segments of bacteriophage phi 6 in vitro. J Virol. 1983 Apr;46(1):196–203. doi: 10.1128/jvi.46.1.196-203.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Georgopoulos D. E., Leibowitz M. J. Nucleotide phosphotransferase, nucleotide kinase and inorganic pyrophosphatase activities of killer virions of yeast. Yeast. 1987 Jun;3(2):117–129. doi: 10.1002/yea.320030208. [DOI] [PubMed] [Google Scholar]
  8. Gottlieb P., Strassman J., Bamford D. H., Mindich L. Production of a polyhedral particle in Escherichia coli from a cDNA copy of the large genomic segment of bacteriophage phi 6. J Virol. 1988 Jan;62(1):181–187. doi: 10.1128/jvi.62.1.181-187.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gottlieb P., Strassman J., Frucht A., Qiao X. Y., Mindich L. In vitro packaging of the bacteriophage phi 6 ssRNA genomic precursors. Virology. 1991 Apr;181(2):589–594. doi: 10.1016/0042-6822(91)90892-f. [DOI] [PubMed] [Google Scholar]
  10. Gottlieb P., Strassman J., Qiao X. Y., Frucht A., Mindich L. In vitro replication, packaging, and transcription of the segmented double-stranded RNA genome of bacteriophage phi 6: studies with procapsids assembled from plasmid-encoded proteins. J Bacteriol. 1990 Oct;172(10):5774–5782. doi: 10.1128/jb.172.10.5774-5782.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Guo P., Peterson C., Anderson D. Prohead and DNA-gp3-dependent ATPase activity of the DNA packaging protein gp16 of bacteriophage phi 29. J Mol Biol. 1987 Sep 20;197(2):229–236. doi: 10.1016/0022-2836(87)90121-5. [DOI] [PubMed] [Google Scholar]
  12. Kapuler A. M., Mendelsohn N., Klett H., Acs G. Four base-specific nucleoside 5'-triphosphatases in the subviral core of reovirus. Nature. 1970 Mar 28;225(5239):1209–1213. doi: 10.1038/2251209a0. [DOI] [PubMed] [Google Scholar]
  13. Mindich L. Bacteriophage phi 6: a unique virus having a lipid-containing membrane and a genome composed of three dsRNA segments. Adv Virus Res. 1988;35:137–176. doi: 10.1016/s0065-3527(08)60710-1. [DOI] [PubMed] [Google Scholar]
  14. Mindich L., Bamford D., Goldthwaite C., Laverty M., Mackenzie G. Isolation of nonsense mutants of lipid-containing bacteriophage PRD1. J Virol. 1982 Dec;44(3):1013–1020. doi: 10.1128/jvi.44.3.1013-1020.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mindich L., Davidoff-Abelson R. The characterization of a 120 S particle formed during phi 6 infection. Virology. 1980 Jun;103(2):386–391. doi: 10.1016/0042-6822(80)90197-x. [DOI] [PubMed] [Google Scholar]
  16. Mindich L., Sinclair J. F., Cohen J. The morphogenesis of bacteriophage phi6: particles formed by nonsense mutants. Virology. 1976 Nov;75(1):224–231. doi: 10.1016/0042-6822(76)90021-0. [DOI] [PubMed] [Google Scholar]
  17. Semancik J. S., Vidaver A. K., Van Etten J. L. Characterization of segmented double-helical RNA from bacteriophage phi6. J Mol Biol. 1973 Aug 25;78(4):617–625. doi: 10.1016/0022-2836(73)90283-0. [DOI] [PubMed] [Google Scholar]
  18. Sinclair J. F., Cohen J., Mindich L. The isolation of suppressible nonsence mutants of bacteriophage phi6. Virology. 1976 Nov;75(1):198–208. [PubMed] [Google Scholar]
  19. Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]
  20. Testa D., Banerjee A. K. Nucleoside diphosphate kinase activity in purified cores of vesicular stomatitis virus. J Biol Chem. 1979 Sep 25;254(18):9075–9079. [PubMed] [Google Scholar]
  21. Usala S. J., Brownstein B. H., Haselkorn R. Displacement of parental RNA strands during in vitro transcription by bacteriophage phi 6 nucleocapsids. Cell. 1980 Apr;19(4):855–862. doi: 10.1016/0092-8674(80)90076-8. [DOI] [PubMed] [Google Scholar]
  22. Vidaver A. K., Koski R. K., Van Etten J. L. Bacteriophage phi6: a Lipid-Containing Virus of Pseudomonas phaseolicola. J Virol. 1973 May;11(5):799–805. doi: 10.1128/jvi.11.5.799-805.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Walker J. E., Saraste M., Runswick M. J., Gay N. J. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1982;1(8):945–951. doi: 10.1002/j.1460-2075.1982.tb01276.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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