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. 1980 Mar;17(3):322–326. doi: 10.1128/aac.17.3.322

Effects of sulfamethoxazole and trimethoprim on human neutrophil and lymphocyte functions in vitro: in vivo effects of co-trimoxazole.

R Anderson, G Grabow, R Oosthuizen, A Theron, A J Van Rensburg
PMCID: PMC283784  PMID: 7425598

Abstract

The effects of sulfamethoxazole and trimethoprim individually and in combination on in vitro neutrophil random migration, chemotaxis to autologous endotoxin-activated serum and the synthetic chemotactic tripeptide N-formyl-L-methionyl-L-leucyl-L-phenylalanine, phagocytosis and postphagocytic Nitro Blue Tetrazolium reduction, glycolysis, hexose monophosphate shunt activity, myeloperoxidase-mediated protein iodination, hydrogen peroxide production, and degranulation were assessed. The effects on lymphocyte mitogen-induced transformation were also evaluated. It was found that the test agents individually and in combination at high concentrations (> 100 microgram/ml) caused the inhibition of neutrophil postphagocytic myeloperoxidase-mediated protein iodination, which was related to the interference with H2O2 formation as the enzyme per se was unaffected. Both agents caused the inhibition of lymphocyte transformation at high concentrations (> 100 microgram/ml). In vivo studies before and after the ingestion of co-trimoxazole by three individuals showed no inhibition of any of the neutrophil functions tested. The inhibition of lymphocyte transformation was observed in one individual after the ingestion of the chemotherapeutic agent. These findings indicate that the concentrations which inhibit neutrophil H2O2 production and lymphocyte transformation in vitro are not attainable in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson R., van Rensburg A. J. The in vitro effects of propranolol and atenolol on neutrophil motility and post-phagocytic metabolic activity. Immunology. 1979 May;37(1):15–24. [PMC free article] [PubMed] [Google Scholar]
  2. Arvilommi H., Vuori M., Salmi A. Immunosuppression by co-trimoxazole. Br Med J. 1972 Sep 23;3(5829):761–762. doi: 10.1136/bmj.3.5829.761-b. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Arvilommi H., Vuori M., Salmi A. Sulphamethoxazole-trimethoprim: effect on antibody response in man. Chemotherapy. 1976;22(1):37–42. doi: 10.1159/000221908. [DOI] [PubMed] [Google Scholar]
  4. Bushby S. R., Hitchings G. H. Trimethoprim, a sulphonamide potentiator. Br J Pharmacol Chemother. 1968 May;33(1):72–90. doi: 10.1111/j.1476-5381.1968.tb00475.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gaylarde P. M., Sarkany I. Suppression of thymidine uptake of human lymphocytes by co-trimoxazole. Br Med J. 1972 Jul 15;3(5819):144–146. doi: 10.1136/bmj.3.5819.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ghilchik M. W., Morris A. S., Reeves D. S. Immunosuppressive powers of the antibacterial agent trimethoprim. Nature. 1970 Jul 25;227(5256):393–394. doi: 10.1038/227393a0. [DOI] [PubMed] [Google Scholar]
  7. Kobayashi Y., Amano D., Ueda K., Kagosaki Y., Usui T. Treatment of seven cases of chronic granulomatous disease with sulfamethoxazole-trimethoprim (SMX-TMP). Eur J Pediatr. 1978 Apr 20;127(4):247–254. doi: 10.1007/BF00493540. [DOI] [PubMed] [Google Scholar]
  8. Paul B. B., Selvaraj R. J., Sbarra A. J. A sensitive assay method for peroxidases from various sources. J Reticuloendothel Soc. 1978 May;23(5):407–410. [PubMed] [Google Scholar]
  9. Root R. K., Metcalf J., Oshino N., Chance B. H2O2 release from human granulocytes during phagocytosis. I. Documentation, quantitation, and some regulating factors. J Clin Invest. 1975 May;55(5):945–955. doi: 10.1172/JCI108024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Root R. K., Stossel T. P. Myeloperoxidase-mediated iodination by granulocytes. Intracellular site of operation and some regulating factors. J Clin Invest. 1974 May;53(5):1207–1215. doi: 10.1172/JCI107667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sher R., Anderson R., Rabson A. R., Koornhof H. J. Standardisation of the nitro-blue tetrazolium test and factors affecting its clinical application. S Afr Med J. 1974 Feb 9;48(6):209–212. [PubMed] [Google Scholar]
  12. WOOD H. G., KATZ J., LANDAU B. R. ESTIMATION OF PATHWAYS OF CARBOHYDRATE METABOLISM. Biochem Z. 1963;338:809–847. [PubMed] [Google Scholar]

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