Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1980 Mar;17(3):470–473. doi: 10.1128/aac.17.3.470

In vitro susceptibility of 104 clinical isolates of Haemophilus influenzae to moxalactam (LY127935), ampicillin, chloramphenicol, and ticarcillin.

E O Mason Jr, S L Kaplan, D C Anderson, D B Hinds, R D Feigin
PMCID: PMC283811  PMID: 6448579

Abstract

A total of 104 strains of Haemophilus influenzae isolated from pediatric patients over a 1-year period were tested for susceptibility to moxalactam (LY127935), ampicillin, chloramphenicol, and ticarcillin. Of these strains, 30 produced beta-lactamase. LY127935 inhibited 99% of the strains at a concentration of 0.125 microgram/ml; the remaining strain was inhibited by this antibiotic at 0.25 microgram/ml. beta-Lactamase-producing strains were inhibited by ampicillin at greater than or equal to 2 microgram/ml. beta-Lactamase-negative strains were all inhibited by ampicillin at less than or equal to 1 microgram/ml, except for one nontypable strain which required 2 microgram of ampicillin per ml for inhibition. All strains were susceptible to chloramphenicol at less than or equal to 4 microgram/ml. beta-Lactamase-producing strains were less susceptible (geometric mean = 4.702 microgram/ml) to ticarcillin than were strains which did not produce beta-lactamase (geometric mean = 0.331 microgram/ml). LY127935 susceptibility was not influenced by increasing inoculum size, as ws ampicillin susceptibility. Combinations of LY127935 and chloramphenicol or ampicillin were not antagonistic in vitro.

Full text

PDF
473

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barza M., Tally F. P., Jacobus N. V., Gorbach S. L. In vitro activity of LY127935. Antimicrob Agents Chemother. 1979 Sep;16(3):287–292. doi: 10.1128/aac.16.3.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brinkley A. W., Huber T. W. Method for evaluating broth culture media: application to Haemophilus. J Clin Microbiol. 1978 Nov;8(5):520–524. doi: 10.1128/jcm.8.5.520-524.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Escamilla J. Susceptibility of Haemophilus influenza to ampicillin as determined by use of a modified, one-minute beta-lactamase test. Antimicrob Agents Chemother. 1976 Jan;9(1):196–198. doi: 10.1128/aac.9.1.196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kammer R. B., Preston D. A., Turner J. R., Hawley L. C. Rapid detection of ampicillin-resistant Haemophilus influenzae and their susceptibility to sixteen antibiotics. Antimicrob Agents Chemother. 1975 Jul;8(1):91–94. doi: 10.1128/aac.8.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Katz S. L. Ampicillin-resistant Hemophilus influenzae type b: a status report. Pediatrics. 1975 Jan;55(1):6–8. [PubMed] [Google Scholar]
  6. Kilian M. A rapid method for the differentiation of Haemophilus strains. The porphyrin test;. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Dec;82(6):835–842. doi: 10.1111/j.1699-0463.1974.tb02381.x. [DOI] [PubMed] [Google Scholar]
  7. Lau W. K., Young L. S., Black R. E., Winston D. J., Linne S. R., Weinstein R. J., Hewitt W. L. Comparative efficacy and toxicity of amikacin/carbenicillin versus gentamicin/carbenicillin in leukopenic patients: a randomized prospective trail. Am J Med. 1977 Jun;62(6):959–966. doi: 10.1016/0002-9343(77)90669-6. [DOI] [PubMed] [Google Scholar]
  8. Myhre E. B. Typing of Haemophilus influenzae by counterimmunoelectrophoresis. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Apr;82(2):164–166. doi: 10.1111/j.1699-0463.1974.tb02308.x. [DOI] [PubMed] [Google Scholar]
  9. Neu H. C., Aswapokee N., Fu K. P., Aswapokee P. Antibacterial activity of a new 1-oxa cephalosporin compared with that of other beta-lactam compounds. Antimicrob Agents Chemother. 1979 Aug;16(2):141–149. doi: 10.1128/aac.16.2.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Overturf G. D., Steinberg E. A., Underman A. E., Wilkins J., Leedom J. M., Mathies A. W., Jr, Wehrle P. F. Comparative trial of carbenicillin and ampicillin therapy for purulent meningitis. Antimicrob Agents Chemother. 1977 Mar;11(3):420–426. doi: 10.1128/aac.11.3.420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Trager G. M., White G. W., Zimelis V. M., Panwalker A. P. LY-127935: a novel beta-lactam antibiotic with unusual antibacterial activity. Antimicrob Agents Chemother. 1979 Sep;16(3):297–300. doi: 10.1128/aac.16.3.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ward J. I., Tsai T. F., Filice G. A., Fraser D. W. Prevalence of ampicillin- and chloramphenicol-resistant strains of Haemophilus influenzae causing meningitis and bacteremia: national survey of hospital laboratories. J Infect Dis. 1978 Sep;138(3):421–424. doi: 10.1093/infdis/138.3.421. [DOI] [PubMed] [Google Scholar]
  13. Wise R., Andrews J. M., Bedford K. A. LY127935, a novel oxa-beta-lactam: an in vitro comparison with other beta-lactam antibiotics. Antimicrob Agents Chemother. 1979 Sep;16(3):341–345. doi: 10.1128/aac.16.3.341. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES