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. 1980 Jul;18(1):130–136. doi: 10.1128/aac.18.1.130

Examination of parameters affecting human interferon production with microcarrier-grown fibroblast cells.

D J Giard, R J Fleischaker
PMCID: PMC283952  PMID: 6158290

Abstract

Various parameters were examined for their effects on interferon production with human fibroblast cells (FS-4) grown on microcarriers, using a superinduction procedure. Optimal concentrations of cycloheximide and actinomycin D during induction were 10 micrograms/ml, respectively. Cells required 5 to 6 h of exposure to cycloheximide and at least 2 h of exposure to actinomycin D to achieve maximum yields. FS-4 cells were found to grow well at low serum concentrations (2.5 and 5%) and actually produced higher yields of interferon than cells grown at higher serum concentration (10 and 20%). Kinetics of interferon production at various temperatures revealed that significantly higher yields could be obtained at 34 degrees C than at either 37 or 30 degrees C. Priming cells for 16 h with 50 U of interferon per ml resulted in consistently higher yields of interferon. By modifying the superinduction procedure in accordance with the above findings, it is now possible to consistently obtain interferon yields of greater than 20,000 international units per 10(6) cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arvin A. M., Feldman S., Merigan T. C. Human leukocyte interferon in the treatment of varicella in children with cancer: a preliminary controlled trial. Antimicrob Agents Chemother. 1978 Apr;13(4):605–607. doi: 10.1128/aac.13.4.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Billiau A., Joniau M., De Somer P. Mass production of human interferon in diploid cells stimulated by poly-I:C. J Gen Virol. 1973 Apr;19(1):1–8. doi: 10.1099/0022-1317-19-1-1. [DOI] [PubMed] [Google Scholar]
  3. Giard D. J., Loeb D. H., Thilly W. G., Wang D. I., Levine D. W. Human interferon production with diploid fibroblast cells grown on microcarriers. Biotechnol Bioeng. 1979 Mar;21(3):433–442. doi: 10.1002/bit.260210306. [DOI] [PubMed] [Google Scholar]
  4. Giard D. J., Thilly W. G., Wang D. I., Levine D. W. Virus production with a newly developed microcarrier system. Appl Environ Microbiol. 1977 Dec;34(6):668–672. doi: 10.1128/aem.34.6.668-672.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Havell E. A., Vilcek J. Production of high-titered interferon in cultures of human diploid cells. Antimicrob Agents Chemother. 1972 Dec;2(6):476–484. doi: 10.1128/aac.2.6.476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Horoszewicz J. S., Leong S. S., Ito M., Buffett R. F., Karakousis C., Holyoke E., Job L., Dölen J. G., Carter W. A. Human fibroblast interferon in human neoplasia: clinical and laboratory study. Cancer Treat Rep. 1978 Nov;62(11):1899–1906. [PubMed] [Google Scholar]
  7. Levine D. W., Wong J. S., Wang D. I., Thilly W. G. Microcarrier cell culture: new methods for research-scale application. Somatic Cell Genet. 1977 Mar;3(2):149–155. doi: 10.1007/BF01551811. [DOI] [PubMed] [Google Scholar]
  8. Merigan T. C., Rand K. H., Pollard R. B., Abdallah P. S., Jordan G. W., Fried R. P. Human leukocyte interferon for the treatment of herpes zoster in patients with cancer. N Engl J Med. 1978 May 4;298(18):981–987. doi: 10.1056/NEJM197805042981801. [DOI] [PubMed] [Google Scholar]
  9. Merigan T. C., Reed S. E., Hall T. S., Tyrrell D. A. Inhibition of respiratory virus infection by locally applied interferon. Lancet. 1973 Mar 17;1(7803):563–567. doi: 10.1016/s0140-6736(73)90714-9. [DOI] [PubMed] [Google Scholar]
  10. Russell W. C., Newman C., Williamson D. H. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature. 1975 Feb 6;253(5491):461–462. doi: 10.1038/253461a0. [DOI] [PubMed] [Google Scholar]
  11. SANFORD K. K., EARLE W. R., EVANS V. J., WALTZ H. K., SHANNON J. E. The measurement of proliferation in tissue cultures by enumeration of cell nuclei. J Natl Cancer Inst. 1951 Feb;11(4):773–795. [PubMed] [Google Scholar]
  12. Schneider E. L., Stanbridge E. J., Epstein C. J. Incorporation of 3H-uridine and 3H-uracil into RNA: a simple technique for the detection of mycoplasma contamination of cultured cells. Exp Cell Res. 1974 Mar 15;84(1):311–318. doi: 10.1016/0014-4827(74)90411-x. [DOI] [PubMed] [Google Scholar]
  13. Strander H., Cantell K. Studies on antiviral and antitumor effects of human leukocyte interferon in vitro and in vivo. In Vitro Monogr. 1974;(3):49–56. [PubMed] [Google Scholar]
  14. Tan Y. H., Armstrong J. A., Ke Y. H., Ho M. Regulation of cellular interferon production: enhancement by antimetabolites. Proc Natl Acad Sci U S A. 1970 Sep;67(1):464–471. doi: 10.1073/pnas.67.1.464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vilcek J., Ng M. H. Post-transcriptional control of interferon synthesis. J Virol. 1971 May;7(5):588–594. doi: 10.1128/jvi.7.5.588-594.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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