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. 1986 May;29(5):833–837. doi: 10.1128/aac.29.5.833

Transfer of amikacin resistance by closely related plasmids in members of the family Enterobacteriaceae isolated in Chile.

G T Van Nhieu, F W Goldstein, M E Pinto, J F Acar, E Collatz
PMCID: PMC284162  PMID: 3015007

Abstract

During a 9-month period when amikacin was the sole aminoglycoside used clinically in a hospital in Santiago, Chile, resistance to amikacin and other antibiotics was encountered in 42 strains of the family Enterobacteriaceae, including Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii, Enterobacter cloacae, Serratia marcescens, and Serratia liquefaciens. Amikacin resistance was transferable by conjugation and carried by IncM plasmids ranging in size from ca. 48.4 to 58.1 kilobase pairs. The plasmids had ca. 70 to 80% of their structure in common, as judged after digestion with restriction endonucleases. The resistance was mediated by a 6' aminoglycoside acetyltransferase. We conclude that selective pressure has favored the dissemination of a wide-host-range amikacin resistance plasmid and its derivatives.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acar J. F., Witchitz J. L., Goldstein F., Talbot J. N., Le Goffic F. Suceptibility of aminoglycoside-resistant gram-negative bacilli to amikacin: delineation of individual resistance patterns. J Infect Dis. 1976 Nov;134(Suppl):S280–S285. doi: 10.1093/infdis/135.supplement_2.s280. [DOI] [PubMed] [Google Scholar]
  2. Betts R. F., Valenti W. M., Chapman S. W., Chonmaitree T., Mowrer G., Pincus P., Messner M., Robertson R. Five-year surveillance of aminoglycoside usage in a university hospital. Ann Intern Med. 1984 Feb;100(2):219–222. doi: 10.7326/0003-4819-100-2-219. [DOI] [PubMed] [Google Scholar]
  3. Chabbert Y. A., Scavizzi M. R., Witchitz J. L., Gerbaud G. R., Bouanchaud D. H. Incompatibility groups and the classification of fi - resistance factors. J Bacteriol. 1972 Nov;112(2):666–675. doi: 10.1128/jb.112.2.666-675.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Datta N., Hedges R. W. Compatibility groups among fi - R factors. Nature. 1971 Nov 26;234(5326):222–223. doi: 10.1038/234222a0. [DOI] [PubMed] [Google Scholar]
  5. Davies J., Smith D. I. Plasmid-determined resistance to antimicrobial agents. Annu Rev Microbiol. 1978;32:469–518. doi: 10.1146/annurev.mi.32.100178.002345. [DOI] [PubMed] [Google Scholar]
  6. Foster T. J. Plasmid-determined resistance to antimicrobial drugs and toxic metal ions in bacteria. Microbiol Rev. 1983 Sep;47(3):361–409. doi: 10.1128/mr.47.3.361-409.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Haas M. J., Dowding J. E. Aminoglycoside-modifying enzymes. Methods Enzymol. 1975;43:611–628. doi: 10.1016/0076-6879(75)43124-x. [DOI] [PubMed] [Google Scholar]
  8. Kawabe H., Naito T., Mitsuhashi S. Acetylation of amikacin, a new semisynthetic antibiotic, by Pseudomonas aeruginosa carrying an R factor. Antimicrob Agents Chemother. 1975 Jan;7(1):50–54. doi: 10.1128/aac.7.1.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kawaguchi H., Naito T., Nakagawa S., Fujisawa K. I. BB-K 8, a new semisynthetic aminoglycoside antibiotic. J Antibiot (Tokyo) 1972 Dec;25(12):695–708. doi: 10.7164/antibiotics.25.695. [DOI] [PubMed] [Google Scholar]
  10. Labigne-Roussel A., Gerbaud G., Courvalin P. Translocation of sequences encoding antibiotic resistance from the chromosome to a receptor plasmid in Salmonella ordonez. Mol Gen Genet. 1981;182(3):390–408. doi: 10.1007/BF00293927. [DOI] [PubMed] [Google Scholar]
  11. Levine J. F., Maslow M. J., Leibowitz R. E., Pollock A. A., Hanna B. A., Schaefler S., Simberkoff M. S., Rahal J. J., Jr Amikacin-resistant gram-negative bacilli: correlation of occurrence with amikacin use. J Infect Dis. 1985 Feb;151(2):295–300. doi: 10.1093/infdis/151.2.295. [DOI] [PubMed] [Google Scholar]
  12. Meyer J. F., Nies B. A., Wiedemann B. Amikacin resistance mediated by multiresistance transposon Tn2424. J Bacteriol. 1983 Aug;155(2):755–760. doi: 10.1128/jb.155.2.755-760.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Meyer J. F., Wiedemann B. Characterization of aminoglycoside 6'-N-acetyltransferases [AAC(6')] from gram-negative bacteria and Streptomyces kanamyceticus. J Antimicrob Chemother. 1985 Mar;15(3):271–282. doi: 10.1093/jac/15.3.271. [DOI] [PubMed] [Google Scholar]
  14. Moody M. M., de Jongh C. A., Schimpff S. C., Tillman G. L. Long-term amikacin use. Effects on aminoglycoside susceptibility patterns of gram-negative bacilli. JAMA. 1982 Sep 10;248(10):1199–1202. doi: 10.1001/jama.248.10.1199. [DOI] [PubMed] [Google Scholar]
  15. Portnoy D. A., Moseley S. L., Falkow S. Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun. 1981 Feb;31(2):775–782. doi: 10.1128/iai.31.2.775-782.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Price K. E., Kresel P. A., Farchione L. A., Siskin S. B., Karpow S. A. Epidemiological studies of aminoglycoside resistance in the U.S.A. J Antimicrob Chemother. 1981 Jul;8 (Suppl A):89–105. doi: 10.1093/jac/8.suppl_a.89. [DOI] [PubMed] [Google Scholar]
  17. Radika K., Northrop D. B. Purification of two forms of kanamycin acetyltransferase from Escherichia coli. Arch Biochem Biophys. 1984 Aug 15;233(1):272–285. doi: 10.1016/0003-9861(84)90626-x. [DOI] [PubMed] [Google Scholar]
  18. Sanson-le Pors M. J., Casin I. M., Collatz E. Plasmid-mediated aminoglycoside phosphotransferases in Haemophilus ducreyi. Antimicrob Agents Chemother. 1985 Aug;28(2):315–319. doi: 10.1128/aac.28.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Witchitz J. L. Epidemiological aspects of aminoglycoside resistance in France. J Antimicrob Chemother. 1981 Jul;8 (Suppl A):71–82. doi: 10.1093/jac/8.suppl_a.71. [DOI] [PubMed] [Google Scholar]
  20. Witchitz J. L., Golstein F. W., Pangon B., Christol D., Acar J. F. Phénotypes de résistance aux aminosides et sensibilité à l'amikacine en milieu hospitalier. Nouv Presse Med. 1979 Oct 31;8(42):3417–3420. [PubMed] [Google Scholar]

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