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. 1994 Oct;38(10):2460–2464. doi: 10.1128/aac.38.10.2460

gyrA mutations in quinolone-resistant isolates of the fish pathogen Aeromonas salmonicida.

H Oppegaard 1, H Sørum 1
PMCID: PMC284763  PMID: 7840589

Abstract

gyrA mutations in quinolone-resistant isolates of Aeromonas salmonicida have been detected by using PCR to amplify the quinolone resistance-determining region of gyrA and subsequent cloning and sequencing of PCR products. Comparison of nucleotide and derived amino acid sequences of PCR products from quinolone-susceptible and -resistant bacteria revealed a serine 83-to-isoleucine substitution in the gyrase A protein of resistant isolates. One of the resistant isolates differed from the other by a two- to fourfold-higher MIC of the fluoroquinolone enrofloxacin and carried an additional alanine 67-to-glycine substitution, which may contribute to the higher level of resistance.

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Selected References

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