Abstract
In Staphylococcus aureus, penicillinase remaining cell-bound (60 to 75% of original total) after treatment with citrate does not become exopenicillinase. Exopenicillinase in these cells appears only under conditions permitting de novo penicillinase synthesis. By use of 14C-labeled cells, it was shown that exopenicillinase consists of newly synthesized molecules which have not equilibrated with preformed membrane-bound enzyme.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- COLLINS J. F. THE DISTRIBUTION AND FORMATION OF PENICILLINASE IN A BACTERIAL POPULATION OF BACILLUS LICHENIFORMIS. J Gen Microbiol. 1964 Mar;34:363–377. doi: 10.1099/00221287-34-3-363. [DOI] [PubMed] [Google Scholar]
- Chesbro W. R., Lampen J. O. Characteristics of secretion of penicillinase, alkaline phosphatase, and nuclease by Bacillus species. J Bacteriol. 1968 Aug;96(2):428–437. doi: 10.1128/jb.96.2.428-437.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coles N. W., Gross R. Influence of organic anions on the liberation of penicillinase from Staphylococcus aureus. Biochem J. 1967 Mar;102(3):748–752. doi: 10.1042/bj1020748. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coles N. W., Gross R. Liberation of surface-located penicillinase from Staphylococcus aureus. Biochem J. 1967 Mar;102(3):742–747. doi: 10.1042/bj1020742. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KUSHNER D. J., POLLOCK M. R. The location of cell-bound penicillinase in Bacillus subtilis. J Gen Microbiol. 1961 Oct;26:255–265. doi: 10.1099/00221287-26-2-255. [DOI] [PubMed] [Google Scholar]
- Lampen J. O. Cell-bound penicillinase of Bacillus licheniformis; properties and purification. J Gen Microbiol. 1967 Aug;48(2):249–259. doi: 10.1099/00221287-48-2-249. [DOI] [PubMed] [Google Scholar]
- Richmond M. H. New type of restriction to the expression of a structural gene in bacteria. Nature. 1967 Dec 23;216(5121):1191–1192. doi: 10.1038/2161191a0. [DOI] [PubMed] [Google Scholar]