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. 1969 May;98(2):737–743. doi: 10.1128/jb.98.2.737-743.1969

Surface Features of Bacillus polymyxa Spores as Revealed by Scanning Electron Microscopy

Judith A Murphy 1, L Leon Campbell 1
PMCID: PMC284879  PMID: 4891267

Abstract

The surface features of Bacillus polymyxa spores were compared by use of thin sections, carbon replicas, and the scanning electron microscope. Some features of the characteristic ridges, previously reported in ultrathin sections and carbon replicas of spores of this species, were more clearly revealed with the scanning electron microscope. A three-dimensional image is provided because of the greater depth of focus possible with this instrument. End-on views of B. polymyxa spores readily illustrate the polygonal porelike structure present.

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Selected References

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  1. ANINGA S., VAN DEN HOOFF A. An electron microscope study on the shape of the spores of Bacillus polymyxa. Antonie Van Leeuwenhoek. 1956;22(4):327–330. doi: 10.1007/BF02538345. [DOI] [PubMed] [Google Scholar]
  2. BRADLEY D. E., FRANKLIN J. G. Electron microscope survey of the surface configuration of spores of the genus Bacillus. J Bacteriol. 1958 Dec;76(6):618–630. doi: 10.1128/jb.76.6.618-630.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BRADLEY D. E., WILLIAMS D. J. An electron microscope study of the spores of some species of the genus Bacillus using carbon replicas. J Gen Microbiol. 1957 Aug;17(1):75–79. doi: 10.1099/00221287-17-1-75. [DOI] [PubMed] [Google Scholar]
  4. DE BOER W. E., SPIT B. J. A NEW TYPE OF BACTERIAL CELL WALL STRUCTURE REVEALED BY REPLICA TECHNIQUE. Antonie Van Leeuwenhoek. 1964;30:239–248. doi: 10.1007/BF02046729. [DOI] [PubMed] [Google Scholar]
  5. DONDERO N. C., HOLBERT P. E. The endospore of Bacillus polymyxa. J Bacteriol. 1957 Jul;74(1):43–47. doi: 10.1128/jb.74.1.43-47.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ellar D. J., Lundgren D. G. Fine structure of sporulation in Bacillus cereus grown in a chemically defined medium. J Bacteriol. 1966 Dec;92(6):1748–1764. doi: 10.1128/jb.92.6.1748-1764.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fitz-James P. C. MORPHOLOGY OF SPORE DEVELOPMENT IN CLOSTRIDIUM PECTINOVORUM. J Bacteriol. 1962 Jul;84(1):104–114. doi: 10.1128/jb.84.1.104-114.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Freer J. H., Levinson H. S. Fine structure of Bacillus megaterium during microcycle sporogenesis. J Bacteriol. 1967 Aug;94(2):441–457. doi: 10.1128/jb.94.2.441-457.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HOLBERT P. E. An effective method of preparing sections of Bacillus polymyxa sporangia and spores for electron microscopy. J Biophys Biochem Cytol. 1960 Apr;7:373–376. doi: 10.1083/jcb.7.2.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KELLENBERGER E., RYTER A., SECHAUD J. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J Biophys Biochem Cytol. 1958 Nov 25;4(6):671–678. doi: 10.1083/jcb.4.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. OHYE D. F., MURRELL W. G. Formation and structure of the spore of Bacillus coagulans. J Cell Biol. 1962 Jul;14:111–123. doi: 10.1083/jcb.14.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Powers E. M. Method for obtaining free bacterial spores of Bacillus subtilis var. niger. Appl Microbiol. 1968 Jan;16(1):180–181. doi: 10.1128/am.16.1.180-181.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. VENABLE J. H., COGGESHALL R. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY. J Cell Biol. 1965 May;25:407–408. doi: 10.1083/jcb.25.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. WARTH A. D., OHYE D. F., MURRELL W. G. The composition and structure of bacterial spores. J Cell Biol. 1963 Mar;16:579–592. doi: 10.1083/jcb.16.3.579. [DOI] [PMC free article] [PubMed] [Google Scholar]

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