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. 1970 Feb;101(2):618–627. doi: 10.1128/jb.101.2.618-627.1970

Comparative Ultrastructure of the Thiobacilli

J M Shively a,1, G L Decker a, J W Greenawalt a
PMCID: PMC284949  PMID: 5413830

Abstract

The ultrastructure of seven Thiobacillus species was studied. The structure of their cell envelopes is similar, if not identical, to that found in other gram-negative bacteria. Obvious differences were noted in the middle layer of the cell envelope of the seven cultures. Polyhedral inclusion bodies were apparent in four of the organisms: T. thioparus, T. neapolitanus, T. intermedius, and T. thiooxidans. Lamellar bodies, similar to those present in certain photosynthetic bacteria were found in a few cells of T. thioparus. Structures resembling mesosomes were discovered in T. dinitrificans. A few cells of T. intermedius possessed paracrystalline bodies. Other inclusions, probably volutin and polysaccharide, were present in some of the cultures.

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Selected References

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  1. Barton L. L., Shively J. M. Thiosulfate utilization by Thiobacillus thiooxidans ATCC 8085. J Bacteriol. 1968 Feb;95(2):720–720. doi: 10.1128/jb.95.2.720-.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cohen-Bazire G., Kunisawa R., Poindexter J. S. The internal membranes of Caulobacter crescentus. J Gen Microbiol. 1966 Feb;42(2):301–308. doi: 10.1099/00221287-42-2-301. [DOI] [PubMed] [Google Scholar]
  3. DUGAN P. R., LUNDGREN D. G. ENERGY SUPPLY FOR THE CHEMOAUTOTROPH FERROBACILLUS FERROOXIDANS. J Bacteriol. 1965 Mar;89:825–834. doi: 10.1128/jb.89.3.825-834.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. De Petris S. Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers. J Ultrastruct Res. 1967 Jul;19(1):45–83. doi: 10.1016/s0022-5320(67)80059-5. [DOI] [PubMed] [Google Scholar]
  5. Gibbs S. P., Sistrom W. R., Worden P. B. The photosynthetic apparatus of Rhodospirillum molischianum. J Cell Biol. 1965 Aug;26(2):395–412. doi: 10.1083/jcb.26.2.395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KELLENBERGER E., RYTER A., SECHAUD J. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J Biophys Biochem Cytol. 1958 Nov 25;4(6):671–678. doi: 10.1083/jcb.4.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MURRAY R. G., STEED P., ELSON H. E. THE LOCATION OF THE MUCOPEPTIDE IN SECTIONS OF THE CELL WALL OF ESCHERICHIA COLI AND OTHER GRAM-NEGATIVE BACTERIA. Can J Microbiol. 1965 Jun;11:547–560. doi: 10.1139/m65-072. [DOI] [PubMed] [Google Scholar]
  9. MURRAY R. G., WATSON S. W. STRUCTURE OF NITROSOCYSTIS OCEANUS AND COMPARISON WITH NITROSOMONAS AND NITROBACTER. J Bacteriol. 1965 Jun;89:1594–1609. doi: 10.1128/jb.89.6.1594-1609.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mahoney R. P., Edwards M. R. Fine Structure of Thiobacillus thiooxidans. J Bacteriol. 1966 Aug;92(2):487–495. doi: 10.1128/jb.92.2.487-495.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Norris J. R., Proctor H. M. Crystalline inclusions in Bacillus thuringiensis. J Bacteriol. 1969 May;98(2):824–826. doi: 10.1128/jb.98.2.824-826.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Remsen C., Lundgren D. G. Electron microscopy of the cell envelope of Ferrobacillus ferrooxidans prepared by freeze-etching and chemical fixation techniques. J Bacteriol. 1966 Dec;92(6):1765–1771. doi: 10.1128/jb.92.6.1765-1771.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Taylor B. F., Hoare D. S. New facultative Thiobacillus and a reevaluation of the heterotrophic potential of Thiobacillus novellus. J Bacteriol. 1969 Oct;100(1):487–497. doi: 10.1128/jb.100.1.487-497.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. VISHNIAC W., SANTER M. The thiobacilli. Bacteriol Rev. 1957 Sep;21(3):195–213. doi: 10.1128/br.21.3.195-213.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Van Caeseele L., Lees H. The ultrastructures of autotrophically and heterotrophically grown Thiobacillus novellus. Can J Microbiol. 1969 Jul;15(7):651–654. doi: 10.1139/m69-116. [DOI] [PubMed] [Google Scholar]
  16. Wang W. S., Lundgren D. G. Poly-beta-hydroxybutyrate in the chemolithotrophic bacterium Ferrobacillus ferrooxidans. J Bacteriol. 1969 Feb;97(2):947–950. doi: 10.1128/jb.97.2.947-950.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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