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. 1970 Apr;102(1):85–96. doi: 10.1128/jb.102.1.85-96.1970

d-Glucaric Acid and Galactaric Acid Catabolism by Agrobacterium tumefaciens

Yung Feng Chang a,1, David Sidney Feingold a
PMCID: PMC284973  PMID: 4314480

Abstract

Cell-free extract (crude extract) of Agrobacterium tumefaciens grown on d-glucuronate or d-glucarate converts d-glucarate and galactarate to a mixture of 2-keto-3-deoxy- and 4-deoxy-5-keto-d-glucarate. These compounds are then converted by partially purified crude extract to an intermediate tentatively identified as 2,5-diketoadipate. The same enzyme preparation further decarboxylates this intermediate to α-ketoglutarate semialdehyde, which is subsequently oxidized in a nicotinamide adenine dinucleotide-dependent reaction to α-ketoglutaric acid. Since A. tumefaciens converts d-glucuronic acid to d-glucarate, a pathway from d-glucuronate to α-ketoglutarate in A. tumefaciens was determined.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASHWELL A., WAHBA A. J., HICKMAN J. A new pathway of uronic acid metabolism. Biochim Biophys Acta. 1958 Oct;30(1):186–187. doi: 10.1016/0006-3002(58)90257-9. [DOI] [PubMed] [Google Scholar]
  2. Adams E., Rosso G. Alpha-ketoglutaric semialdehyde dehydrogenase of Pseudomonas. Properties of the purified enzyme induced by hydroxyproline and of the glucarate-induced and constitutive enzymes. J Biol Chem. 1967 Apr 25;242(8):1802–1814. [PubMed] [Google Scholar]
  3. Ankel H., Feingold D. S. Biosynthesis of uridine diphosphate D-xylose. II. Uridine diphosphate D-glucuronate carboxy-lyase of Cryptococcus laurentii. Biochemistry. 1966 Jan;5(1):182–189. doi: 10.1021/bi00865a024. [DOI] [PubMed] [Google Scholar]
  4. BLUMENTHAL H. J., FISH D. C. Bacterial conversion of D-glucarate to glycerate and pyruvate. Biochem Biophys Res Commun. 1963 May 3;11:239–243. doi: 10.1016/0006-291x(63)90341-3. [DOI] [PubMed] [Google Scholar]
  5. Blumenthal H. J., Jepson T. Asymmetric dehydration of galactarate by bacterial galactarate dehydratase. Biochem Biophys Res Commun. 1964 Oct 14;17(3):282–287. doi: 10.1016/0006-291x(64)90398-5. [DOI] [PubMed] [Google Scholar]
  6. CAVALLINI D., FRONTALI N. Quantitative determination of keto-acids by paper partition chromatography. Biochim Biophys Acta. 1954 Mar;13(3):439–445. doi: 10.1016/0006-3002(54)90351-0. [DOI] [PubMed] [Google Scholar]
  7. CAVALLINI D., FRONTALI N., TOSCHI G. Keto-acid content of human blood and urine. Nature. 1949 Nov 5;164(4175):792–792. doi: 10.1038/164792b0. [DOI] [PubMed] [Google Scholar]
  8. Chang Y. F., Feingold D. S. Hexuronic acid dehydrogenase of Agrobacterium tumefaciens. J Bacteriol. 1969 Sep;99(3):667–673. doi: 10.1128/jb.99.3.667-673.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. DAGLEY S., TRUDGILL P. W. THE METABOLISM OF GALACTARATE, D-GLUCARATE AND VARIOUS PENTOSES BY SPECIES OF PSEUDOMONAS. Biochem J. 1965 Apr;95:48–58. doi: 10.1042/bj0950048. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. ENTNER N., DOUDOROFF M. Glucose and gluconic acid oxidation of Pseudomonas saccharophila. J Biol Chem. 1952 May;196(2):853–862. [PubMed] [Google Scholar]
  11. Farmer J. J., 3rd, Eagon R. G. Aldohexuronic acid catabolism by a soil Aeromonas. J Bacteriol. 1969 Jan;97(1):97–106. doi: 10.1128/jb.97.1.97-106.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KESSLER G., NEUFELD E. F., FEINGOLD D. S., HASSID W. Z. Metabolism of D-glucuronic acid and D-galacturonic acid by Phaseolus aureus seedlings. J Biol Chem. 1961 Feb;236:308–312. [PubMed] [Google Scholar]
  13. KILGORE W. W., STARR M. P. Uronate oxidation by phytopathogenic pseudomonads. Nature. 1959 May 16;183(4672):1412–1413. doi: 10.1038/1831412a0. [DOI] [PubMed] [Google Scholar]
  14. Kohn L. D., Jakoby W. B. Tartaric acid metabolism. 3. The formation of glyceric acid. J Biol Chem. 1968 May 25;243(10):2465–2471. [PubMed] [Google Scholar]
  15. LEVIN D. H., RACKER E. Condensation of arabinose 5-phosphate and phosphorylenol pyruvate by 2-keto-3-deoxy-8-phosphooctonic acid synthetase. J Biol Chem. 1959 Oct;234:2532–2539. [PubMed] [Google Scholar]
  16. LOEWUS F. A., KELLY S. The metabolism of p-galacturonic acid and its methyl ester in the detached ripening strawberry. Arch Biochem Biophys. 1961 Dec;95:483–493. doi: 10.1016/0003-9861(61)90180-1. [DOI] [PubMed] [Google Scholar]
  17. Marsh C. A. Metabolism of d-glucuronolactone in mammalian systems. 2. Conversion of d-glucuronolactone into d-glucaric acid by tissue preparations. Biochem J. 1963 Apr;87(1):82–90. doi: 10.1042/bj0870082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. PREISS J., ASHWELL G. 3-Deoxy-D-glycero-2,5-hexodiulo-sonic acid, a new intermediate in the metabolism of polygalacturonate. Biochem Biophys Res Commun. 1962 Aug 7;8:357–360. doi: 10.1016/0006-291x(62)90007-4. [DOI] [PubMed] [Google Scholar]
  19. SINGH R. M., ADAMS E. ALPHA-KETOGLUTARIC SEMIALDEHYDE: A METABOLIC INTERMEDIATE. Science. 1964 Apr 3;144(3614):67–68. doi: 10.1126/science.144.3614.67. [DOI] [PubMed] [Google Scholar]
  20. SMILEY J. D., ASHWELL G. Uronic acid metabolism in bacteria. III. Purification and properties of D-altronic acid and D-mannonic acid dehydrases in Escherichia coli. J Biol Chem. 1960 Jun;235:1571–1575. [PubMed] [Google Scholar]
  21. SRINIVASAN P. R., SPRINSON D. B. 2-Keto-3-deoxy-D-arabo-heptonic acid 7-phosphate synthetase. J Biol Chem. 1959 Apr;234(4):716–722. [PubMed] [Google Scholar]
  22. STARR M. P., MORAN F. Eliminative split of pectic substances by phytopathogenic soft-rot bacteria. Science. 1962 Mar 16;135(3507):920–921. doi: 10.1126/science.135.3507.920. [DOI] [PubMed] [Google Scholar]
  23. STOOLMILLER A. C., ABELES R. H. FORMATION OF ALPHA-KETOGLUTARATE SEMIALDEHYDE. AN INTRAMOLECULAR DISMUTATION REACTION. Biochem Biophys Res Commun. 1965 May 3;19:438–443. doi: 10.1016/0006-291x(65)90143-9. [DOI] [PubMed] [Google Scholar]
  24. STRECKER H. J. Glutamic dehydrogenase. Arch Biochem Biophys. 1953 Sep;46(1):128–140. doi: 10.1016/0003-9861(53)90176-3. [DOI] [PubMed] [Google Scholar]
  25. Singh R. M., Adams E. Isolation and identification of 2,5-dioxovalerate, an intermediate in the bacterial oxidation of hydroxyproline. J Biol Chem. 1965 Nov;240(11):4352–4356. [PubMed] [Google Scholar]
  26. TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
  27. TSUJINO I., SAITO T. A new unsaturated uronide isolated from alginase hydrolysate. Nature. 1961 Dec 9;192:970–971. doi: 10.1038/192970a0. [DOI] [PubMed] [Google Scholar]
  28. WARAVDEKAR V. S., SASLAW L. D. A method of estimation of 2-deoxyribose. Biochim Biophys Acta. 1957 May;24(2):439–439. doi: 10.1016/0006-3002(57)90224-x. [DOI] [PubMed] [Google Scholar]
  29. WARREN L. Thiobarbituric acid spray reaction for deoxy sugars and sialic acids. Nature. 1960 Apr 16;186:237–237. doi: 10.1038/186237a0. [DOI] [PubMed] [Google Scholar]
  30. WEISSBACH A., HURWITZ J. The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification. J Biol Chem. 1959 Apr;234(4):705–709. [PubMed] [Google Scholar]
  31. ZAJIC J. E. Hexuronic dehydrogenase of Agrobacterium tumefaciens. J Bacteriol. 1959 Nov;78:734–735. doi: 10.1128/jb.78.5.734-735.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]

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