Abstract
The effects of bdellovibrio infection on host permeability and respiration were investigated by measuring respiration rates and the rate of o-nitrophenyl-β-d-galactopyranoside hydrolysis during the course of single infection cycles of Bdellovibrio bacteriovorus strain 109 growing on Escherichia coli ML 35 (lac i−z+y−). The data show that among the very early consequences of parasite attack on the host are an increase in permeability and a general disruption of respiratory activity of the host, and it is suggested that both phenomena stem from early damage to host membrane. The rapid onset of damage after inception of the cycle and the failure of streptomycin to prevent the damage indicate that complete penetration of the parasite into the host is not a requirement for the observed effects. The data also show that bdellovibrio does not use host energy-generating mechanisms for its growth and suggest that the parasites may have a search mechanism that permits them, to some degree, to distinguish between infected and uninfected hosts.
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- Burnham J. C., Hashimoto T., Conti S. F. Electron microscopic observations on the penetration of Bdellovibrio bacteriovorus into gram-negative bacterial hosts. J Bacteriol. 1968 Oct;96(4):1366–1381. doi: 10.1128/jb.96.4.1366-1381.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Seidler R. J., Starr M. P. Factors affecting the intracellular parasitic growth of Bdellovibrio bacteriovorus developing within Escherichia coli. J Bacteriol. 1969 Feb;97(2):912–923. doi: 10.1128/jb.97.2.912-923.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shilo M., Bruff B. Lysis of Gram-negative bacteria by host-independent ectoparasitic Bdellovibrio bacteriovorus isolates. J Gen Microbiol. 1965 Sep;40(3):317–328. doi: 10.1099/00221287-40-3-317. [DOI] [PubMed] [Google Scholar]
- Shilo M. Morphological and physiological aspects of the interaction of Bdellovibrio with host bacteria. Curr Top Microbiol Immunol. 1969;50:174–204. doi: 10.1007/978-3-642-46169-9_6. [DOI] [PubMed] [Google Scholar]
- Simpson F. J., Robinson J. Some energy-producing systems in Bdellovibrio bacteriovorus, strain 6-5-S. Can J Biochem. 1968 Aug;46(8):865–873. doi: 10.1139/o68-129. [DOI] [PubMed] [Google Scholar]
- Stanier R. Y. THE CYTOPHAGA GROUP: A CONTRIBUTION TO THE BIOLOGY OF MYXOBACTERIA. Bacteriol Rev. 1942 Sep;6(3):143–196. doi: 10.1128/br.6.3.143-196.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Starr M. P., Baigent N. L. Parasitic interaction of Bdellovibrio bacteriovorus with other bacteria. J Bacteriol. 1966 May;91(5):2006–2017. doi: 10.1128/jb.91.5.2006-2017.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stolp H. Bdellovibrio bacteriovorus--ein räuberischer Bakterienparisit. Naturwissenschaften. 1968 Feb;55(2):57–63. doi: 10.1007/BF00599479. [DOI] [PubMed] [Google Scholar]
- Varon M., Shil M. Interacton of Bdellovibrio bacteriovorus and host bacteria. I. Kinetic studies of attachment and invasion of Escherichia coli B by Bdellovibrio bacteriovorus. J Bacteriol. 1968 Mar;95(3):744–753. doi: 10.1128/jb.95.3.744-753.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Varon M., Shilo M. Interaction of Bdellovibrio bacteriovorus and host bacteria. II. Intracellular growth and development of Bdellovibrio bacteriovorus in liquid cultures. J Bacteriol. 1969 Jul;99(1):136–141. doi: 10.1128/jb.99.1.136-141.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]