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. 1972 Feb;109(2):784–789. doi: 10.1128/jb.109.2.784-789.1972

Role of Metabolic Energy in the Transport of β-Galactosides by Streptococcus lactis1

E R Kashket a, T H Wilson a
PMCID: PMC285206  PMID: 4621686

Abstract

Streptococcus lactis (ATCC 7962) accumulated thiomethyl-β-galactoside (TMG) and other galactosides against concentration gradients when the cells were supplied with a metabolizable substrate, such as glucose. The accumulated TMG was free and not phosphorylated. In the absence of glucose, TMG rapidly entered the cell to a concentration equal to that of the medium. Agents that uncouple oxidative phosphorylation abolished active transport but not the carrier-facilitated entry of TMG. Evidence that the transport carriers were functional in the absence of glucose or in the presence of uncoupling agents included the demonstration of counterflow, which depends on competitive inhibition for the carrier for exit.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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