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. 1972 Feb;109(2):848–854. doi: 10.1128/jb.109.2.848-854.1972

Chromosome Replication and Cell Division in Escherichia coli at Various Temperatures of Growth

O Pierucci 1
PMCID: PMC285215  PMID: 4550822

Abstract

The effect of temperature on the growth rate and the pattern of chromosome replication during the division cycle of Escherichia coli B/r growing in various media was investigated. The time between divisions, the time for a round of replication (C), and the time between completion of a round and cell division (D) were threefold longer at 21 C than at 37 C. At all temperatures and in all media, D equalled one-half C, suggesting that a common mechanism controls chromosome replication and the progression of the cell toward division after completion of a round of replication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bird R. E., Lark K. G. Chromosome replication in Escherichia coli 15T- at different growth rates: rate of replication of the chromosome and the rate of formation of small pieces. J Mol Biol. 1970 Apr 28;49(2):343–366. doi: 10.1016/0022-2836(70)90249-4. [DOI] [PubMed] [Google Scholar]
  2. Bird R., Lark K. G. Initiation and termination of DNA replication after amino acid starvation of E. coli 15T-. Cold Spring Harb Symp Quant Biol. 1968;33:799–808. doi: 10.1101/sqb.1968.033.01.092. [DOI] [PubMed] [Google Scholar]
  3. Chai N. C., Lark K. G. Cytological studies of deoxyribonucleic acid replication in Escherichia coli 15T-: replication at slow growth rates and after a shift-up into rich medium. J Bacteriol. 1970 Oct;104(1):401–409. doi: 10.1128/jb.104.1.401-409.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark D. J. Regulation of deoxyribonucleic acid replication and cell division in Escherichia coli B-r. J Bacteriol. 1968 Oct;96(4):1214–1224. doi: 10.1128/jb.96.4.1214-1224.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clark D. J. The regulation of DNA replication and cell division in E. coli B-r. Cold Spring Harb Symp Quant Biol. 1968;33:823–838. doi: 10.1101/sqb.1968.033.01.094. [DOI] [PubMed] [Google Scholar]
  6. Cooper S., Helmstetter C. E. Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):519–540. doi: 10.1016/0022-2836(68)90425-7. [DOI] [PubMed] [Google Scholar]
  7. Donachie W. D. Relationship between cell size and time of initiation of DNA replication. Nature. 1968 Sep 7;219(5158):1077–1079. doi: 10.1038/2191077a0. [DOI] [PubMed] [Google Scholar]
  8. Eberle H., Lark K. G. Chromosome replication in Bacillus subtilis cultures growing at different rates. Proc Natl Acad Sci U S A. 1967 Jan;57(1):95–101. doi: 10.1073/pnas.57.1.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fuchs E., Hanawalt P. Isolation and characterization of the DNA replication complex from Escherichia coli. J Mol Biol. 1970 Sep 14;52(2):301–322. doi: 10.1016/0022-2836(70)90032-x. [DOI] [PubMed] [Google Scholar]
  10. HANAWALT P. C., RAY D. S. ISOLATION OF THE GROWING POINT IN THE BACTERIAL CHROMOSOME. Proc Natl Acad Sci U S A. 1964 Jul;52:125–132. doi: 10.1073/pnas.52.1.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Helmstetter C. E. DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):507–518. doi: 10.1016/0022-2836(68)90424-5. [DOI] [PubMed] [Google Scholar]
  12. Helmstetter C. E., Pierucci O. Cell division during inhibition of deoxyribonucleic acid synthesis in Escherichia coli. J Bacteriol. 1968 May;95(5):1627–1633. doi: 10.1128/jb.95.5.1627-1633.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Helmstetter C., Cooper S., Pierucci O., Revelas E. On the bacterial life sequence. Cold Spring Harb Symp Quant Biol. 1968;33:809–822. doi: 10.1101/sqb.1968.033.01.093. [DOI] [PubMed] [Google Scholar]
  14. Inouye M., Guthrie J. P. A mutation which changes a membrane protein of E. coli. Proc Natl Acad Sci U S A. 1969 Nov;64(3):957–961. doi: 10.1073/pnas.64.3.957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kubitschek H. E., Freedman M. L. Chromosome replication and the division cycle of Escherichia coli B-r. J Bacteriol. 1971 Jul;107(1):95–99. doi: 10.1128/jb.107.1.95-99.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pierucci O., Helmstetter C. E. Chromosome replication, protein synthesis and cell division in Escherichia coli. Fed Proc. 1969 Nov-Dec;28(6):1755–1760. [PubMed] [Google Scholar]
  17. Pierucci O. Regulation of cell division in Escherichia coli. Biophys J. 1969 Jan;9(1):90–112. doi: 10.1016/S0006-3495(69)86371-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ryter A., Hirota Y., Jacob F. DNA-membrane complex and nuclear segregation in bacteria. Cold Spring Harb Symp Quant Biol. 1968;33:669–676. doi: 10.1101/sqb.1968.033.01.076. [DOI] [PubMed] [Google Scholar]
  19. SCHAECHTER M., MAALOE O., KJELDGAARD N. O. Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium. J Gen Microbiol. 1958 Dec;19(3):592–606. doi: 10.1099/00221287-19-3-592. [DOI] [PubMed] [Google Scholar]
  20. Siccardi A. G., Shapiro B. M. On the process of cellular division in Escherichia coli. IV. Altered protein composition and turnover of the membranes of thermosensitive mutants defective in chromosomal replication. J Mol Biol. 1971 Mar 28;56(3):475–490. doi: 10.1016/0022-2836(71)90395-0. [DOI] [PubMed] [Google Scholar]
  21. Smith D. W., Hanawalt P. C. Properties of the growing point region in the bacterial chromosome. Biochim Biophys Acta. 1967 Dec 19;149(2):519–531. doi: 10.1016/0005-2787(67)90180-3. [DOI] [PubMed] [Google Scholar]
  22. Sueoka N., Quinn W. G. Membrane attachment of the chromosome replication origin in Bacillus subtilis. Cold Spring Harb Symp Quant Biol. 1968;33:695–705. doi: 10.1101/sqb.1968.033.01.078. [DOI] [PubMed] [Google Scholar]
  23. Urban J. E., Lark K. G. DNA replication in Escherichia coli 15T- growing at 20 degrees C. J Mol Biol. 1971 Jun 28;58(3):711–724. doi: 10.1016/0022-2836(71)90035-0. [DOI] [PubMed] [Google Scholar]
  24. Werner R. Mechanism of DNA replication. Nature. 1971 Apr 30;230(5296):570–572. doi: 10.1038/230570a0. [DOI] [PubMed] [Google Scholar]

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