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. 1973 Feb;113(2):565–571. doi: 10.1128/jb.113.2.565-571.1973

Membrane Lipids of Mycoplasma hominis

Shlomo Rottem 1, Shmuel Razin 1
PMCID: PMC285266  PMID: 4347923

Abstract

Essentially all of the lipids of Mycoplasma hominis (200 μg/mg of cell protein) were found to be located in the cell membrane. Over one-half were neutral lipids incorporated from the growth medium and consisting of 43% free cholesterol, 19% esterified cholesterol, 23% triglycerides, 10% free fatty acids, and small amounts of di- and monoglycerides. The polar lipids accounting for about 40% of the total were synthesized by the organisms. Phosphatidylglycerol was the predominant lipid of this fraction. The minor components, tentatively identified as lysophosphatidylglycerol and phosphatidic acid, seem to represent breakdown products of phosphatidylglycerol. No glycolipids were detected. Being unable to synthesize long-chain fatty acids, M. hominis utilized the fatty acids of the growth medium for polar lipid synthesis, preferentially the saturated ones, so that the polar lipids had highly saturated hydrocarbon chains. It is proposed that the large take up of unsaturated neutral lipids and cholesterol from the medium offsets the marked condensing effect of the saturated polar lipids, although electron paramagnetic resonance spectrometry of spin-labeled fatty acids incorporated into the M. hominis membrane indicated that the lipid region is still more rigid than that of the Acholeplasma laidlawii membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARGAMAN M., RAZIN S. CHOLESTEROL AND CHOLESTEROL ESTERS IN MYCOPLASMA. J Gen Microbiol. 1965 Jan;38:153–160. doi: 10.1099/00221287-38-1-153. [DOI] [PubMed] [Google Scholar]
  2. DITTMER J. C., LESTER R. L. A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS. J Lipid Res. 1964 Jan;5:126–127. [PubMed] [Google Scholar]
  3. Engelman D. M. Lipid bilayer structure in the membrane of Mycoplasma laidlawii. J Mol Biol. 1971 May 28;58(1):153–165. doi: 10.1016/0022-2836(71)90238-5. [DOI] [PubMed] [Google Scholar]
  4. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Ladbrooke B. D., Chapman D. Thermal analysis of lipids, proteins and biological membranes. A review and summary of some recent studies. Chem Phys Lipids. 1969 Dec;3(4):304–356. doi: 10.1016/0009-3084(69)90040-1. [DOI] [PubMed] [Google Scholar]
  7. McElhaney R. N., Tourtellotte M. E. Mycoplasma membrane lipids: variations in fatty acid composition. Science. 1969 Apr 25;164(3878):433–434. doi: 10.1126/science.164.3878.433. [DOI] [PubMed] [Google Scholar]
  8. Metcalfe S. M., Metcalfe J. C., Engelman D. M. Structural comparisons of native and reaggregated membranes from Mycoplasma laidawii and erythrocytes using a fluorescence probe. Biochim Biophys Acta. 1971 Aug 13;241(2):422–430. doi: 10.1016/0005-2736(71)90042-3. [DOI] [PubMed] [Google Scholar]
  9. PARSONS J., WYCOFF H. D. Chromatographic microassay for cholesterol and cholesterol esters. Science. 1957 Feb 22;125(3243):347–348. doi: 10.1126/science.125.3243.347. [DOI] [PubMed] [Google Scholar]
  10. Pollack J. D., Razin S., Pollack M. E., Cleverdon R. C. Fractionation of mycoplasma cells for enzyme localization. Life Sci. 1965 May;4(9):973–977. doi: 10.1016/0024-3205(65)90200-6. [DOI] [PubMed] [Google Scholar]
  11. RAZIN S. OSMOTIC LYSIS OF MYCOPLASMA. J Gen Microbiol. 1963 Dec;33:471–475. doi: 10.1099/00221287-33-3-471. [DOI] [PubMed] [Google Scholar]
  12. Razin S. Structure and function in mycoplasma. Annu Rev Microbiol. 1969;23:317–356. doi: 10.1146/annurev.mi.23.100169.001533. [DOI] [PubMed] [Google Scholar]
  13. Razin S., Tourtellotte M. E., McElhaney R. N., Pollack J. D. Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells. J Bacteriol. 1966 Feb;91(2):609–616. doi: 10.1128/jb.91.2.609-616.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Romano N., Smith P. F., Mayberry W. R. Lipids of a T strain of Mycoplasma. J Bacteriol. 1972 Feb;109(2):565–569. doi: 10.1128/jb.109.2.565-569.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rottem S., Hubbell W. L., Hayflick L., McConnell H. M. Motion of fatty acid spin labels in the plasma membrane of mycoplasma. Biochim Biophys Acta. 1970;219(1):104–113. doi: 10.1016/0005-2736(70)90065-9. [DOI] [PubMed] [Google Scholar]
  16. Rottem S., Muhsam-Peled O., Razin S. Acyl carrier protein in mycoplasmas. J Bacteriol. 1973 Feb;113(2):586–591. doi: 10.1128/jb.113.2.586-591.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rottem S., Panos C. The synthesis of long-chain fatty acids by a cell-free system from Mycoplasma laidlawii A. Biochemistry. 1970 Jan 6;9(1):57–63. doi: 10.1021/bi00803a008. [DOI] [PubMed] [Google Scholar]
  18. Rottem S., Razin S. Isolation of mycoplasma membranes by digitonin. J Bacteriol. 1972 May;110(2):699–705. doi: 10.1128/jb.110.2.699-705.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rottem S., Stein O., Razin S. Reassembly of Mycoplasma membranes disaggregated by detergents. Arch Biochem Biophys. 1968 Apr;125(1):46–56. doi: 10.1016/0003-9861(68)90637-1. [DOI] [PubMed] [Google Scholar]
  20. STERN I., SHAPIRO B. A rapid and simple method for the determination of esterified fatty acids and for total fatty acids in blood. J Clin Pathol. 1953 May;6(2):158–160. doi: 10.1136/jcp.6.2.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shaw N., Smith P. F., Koostra W. L. The lipid composition of Mycoplasma laidlawii strain B. Biochem J. 1968 Apr;107(3):329–333. doi: 10.1042/bj1070329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Shaw N. The detection of lipids on thin-layer chromatograms with the periodate-Schiff reagents. Biochim Biophys Acta. 1968 Oct 22;164(2):435–436. doi: 10.1016/0005-2760(68)90171-9. [DOI] [PubMed] [Google Scholar]
  23. Steim J. M., Tourtellotte M. E., Reinert J. C., McElhaney R. N., Rader R. L. Calorimetric evidence for the liquid-crystalline state of lipids in a biomembrane. Proc Natl Acad Sci U S A. 1969 May;63(1):104–109. doi: 10.1073/pnas.63.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tourtellotte M. E., Branton D., Keith A. Membrane structure: spin labeling and freeze etching of Mycoplasma laidawii. Proc Natl Acad Sci U S A. 1970 Jul;66(3):909–916. doi: 10.1073/pnas.66.3.909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. de Kruyff B., Demel R. A., van Deenen L. L. The effect of cholesterol and epicholesterol incorporation on the permeability and on the phase transition of intact Acholeplasma laidlawii cell membranes and derived liposomes. Biochim Biophys Acta. 1972 Jan 17;255(1):331–347. doi: 10.1016/0005-2736(72)90032-6. [DOI] [PubMed] [Google Scholar]

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