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. 1973 Feb;113(2):576–585. doi: 10.1128/jb.113.2.576-585.1973

Competence for Transfection in Staphylococcus aureus

Jan-Eric Sjöström 1, Martin Lindberg 1, Lennart Philipson 1
PMCID: PMC285268  PMID: 4266172

Abstract

Lysogenicity with phage P11 is a requirement for competence in the presence of calcium ions in Staphylococcus aureus 8325N. The wild-type strain 8325N, lysogenic for the phages P11, P12, and P13, is also competent, but strain 8325-4, a nonlysogenic derivative of strain 8325N, as well as strains 8325-4 (P12) and 8325-4 (P13) could not develop competence. Preincubation of strain 8325-4 with culture filtrates from a competent strain can induce competence, but rabbit anti-P11 serum can neutralize the competence factor. Superinfection of competent strain 8325-4 (P11) with phage P11 at high multiplicities increases the transfection frequency. Uptake of deoxyribonucleic acid by competent cells is dependent on calcium ion concentration, pH, and temperature. Inhibition of energy metabolism or protein synthesis before and during incubation with deoxyribonucleic acid affects the binding and uptake. The ability to develop competence during bacterial growth differs between the wild-type strain (8325N) and a nuclease-deficient mutant (8325N nuc). The wild-type strain has a narrow competence maximum in the early exponential growth phase where no extracellular nuclease activity is produced. The nuc strain shows in addition competence maxima later in the exponential growth phase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALEXANDER M., HEPPEL L. A., HURWITZ J. The purification and properties of micrococcal nuclease. J Biol Chem. 1961 Nov;236:3014–3019. [PubMed] [Google Scholar]
  2. BARNHART B. J., HERRIOTT R. M. PENETRATION OF DEOXYRIBONUCLEIC ACID INTO HEMOPHILUS INFLUENZAE. Biochim Biophys Acta. 1963 Sep 17;76:25–39. [PubMed] [Google Scholar]
  3. Bott K. F., Wilson G. A. Metabolic and nutritional factors influencing the development of competence for transfection of Bacillus subtilis. Bacteriol Rev. 1968 Dec;32(4 Pt 1):370–378. [PMC free article] [PubMed] [Google Scholar]
  4. Brown D. T., Brown N. C., Burlingham B. T. Morphology and physical properties of Staphylococcus bacteriophage P11-M15. J Virol. 1972 Apr;9(4):664–671. doi: 10.1128/jvi.9.4.664-671.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Charpak M., Dedonder R. Production d'un "facteur de compétence" soluble par Bacillus subtilis Marburg ind 168. C R Acad Sci Hebd Seances Acad Sci D. 1965 May 24;260(21):5638–5641. [PubMed] [Google Scholar]
  6. Dobrzański W. T., Kaczanowska J., Osowiecki H. Deoxyribonuclease production in populations of staphylococci. Zentralbl Bakteriol Orig. 1968 Feb;206(1):41–45. [PubMed] [Google Scholar]
  7. Epstein H. T. Factors affecting bacterial competence for transfection and transfection enhancement. Bacteriol Rev. 1968 Dec;32(4 Pt 1):313–319. [PMC free article] [PubMed] [Google Scholar]
  8. JACOBS S. I., WILLIS A. T., GOODBURN G. M. PIGMENT PRODUCTION AND ENZYMATIC ACTIVITY OF STAPHYLOCOCCI: THE DIFFERENTIATION OF PATHOGENS FROM COMMENSALS. J Pathol Bacteriol. 1964 Jan;87:151–156. doi: 10.1002/path.1700870121. [DOI] [PubMed] [Google Scholar]
  9. JYSSUM K., LIE S. GENETIC FACTORS DETERMINING COMPETENCE IN TRANSFORMATION OF NEISSERIA MENINGITIDIS. 1. A PERMANENT LOSS OF COMPETENCE. Acta Pathol Microbiol Scand. 1965;63:306–316. doi: 10.1111/apm.1965.63.2.306. [DOI] [PubMed] [Google Scholar]
  10. KAISER A. D., HOGNESS D. S. The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage lambda-dg. J Mol Biol. 1960 Dec;2:392–415. doi: 10.1016/s0022-2836(60)80050-2. [DOI] [PubMed] [Google Scholar]
  11. Lindberg M., Sjöström J. E., Johansson T. Transformation of chromosomal and plasmid characters in Staphylococcus aureus. J Bacteriol. 1972 Feb;109(2):844–847. doi: 10.1128/jb.109.2.844-847.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  13. NOVICK R. P. ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1963 Oct;33:121–136. doi: 10.1099/00221287-33-1-121. [DOI] [PubMed] [Google Scholar]
  14. NOVICK R. P., RICHMOND M. H. NATURE AND INTERACTIONS OF THE GENETIC ELEMENTS GOVERNING PENICILLINASE SYNTHESIS IN STAPHYLOCOCCUS AUREUS. J Bacteriol. 1965 Aug;90:467–480. doi: 10.1128/jb.90.2.467-480.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Novick R. Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology. 1967 Sep;33(1):155–166. doi: 10.1016/0042-6822(67)90105-5. [DOI] [PubMed] [Google Scholar]
  16. Omenn G. S., Friedman J. Isolation of mutants of Staphylococcus aureus lacking extracellular nuclease activity. J Bacteriol. 1970 Mar;101(3):921–924. doi: 10.1128/jb.101.3.921-924.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Riva S., Polsinelli M. Relationship between competence for transfection and for transformation. J Virol. 1968 Jun;2(6):587–593. doi: 10.1128/jvi.2.6.587-593.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. STUY J. H. Transformability of Haemophilus influenzae. J Gen Microbiol. 1962 Nov;29:537–549. doi: 10.1099/00221287-29-3-537. [DOI] [PubMed] [Google Scholar]
  19. Sjöström J. E., Lindberg M., Philipson L. Transfection of Staphylococcus aureus with bacteriophage deoxyribonucleic acid. J Bacteriol. 1972 Jan;109(1):285–291. doi: 10.1128/jb.109.1.285-291.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Spizizen J., Reilly B. E., Evans A. H. Microbial transformation and transfection. Annu Rev Microbiol. 1966;20:371–400. doi: 10.1146/annurev.mi.20.100166.002103. [DOI] [PubMed] [Google Scholar]
  21. Streips U. N., Welker N. E. Competence-inducing factor of Bacillus stearothermophilus. J Bacteriol. 1971 Jun;106(3):955–959. doi: 10.1128/jb.106.3.955-959.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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