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. 1973 Feb;113(2):724–726. doi: 10.1128/jb.113.2.724-726.1973

Host Specificity of Salmonella typhimurium Deoxyribonucleic Acid Restriction and Modification

Harvey Slocum 1, Herbert W Boyer 1
PMCID: PMC285287  PMID: 4570605

Abstract

The restriction and modification genes of Salmonella typhimurium which lie near the thr locus were transferred to a restrictionless mutant of Escherichia coli. These genes were found to be allelic to the E. coli K, B, and A restriction and modification genes. E. coli recombinants with the restriction and modification host specificity of S. typhimurium restricted phage λ that had been modified by each of the seven known host specificities of E. coli at efficiency of plating levels of about 10−2. Phage λ modified with the S. typhimurium host specificity was restricted by six of the seven E. coli host specificities but not by the RII (fi− R-factor controlled) host specificity. It is proposed that the restriction and modification enzymes of this S. typhimurium host specificity have two substrates, one of which is a substrate for the RII host specificity enzymes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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