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. 1973 Jun;114(3):1040–1044. doi: 10.1128/jb.114.3.1040-1044.1973

Role of Cyclic Adenosine 3′,5′-Monophosphate in the In Vivo Expression of the Galactose Operon of Escherichia coli

Lucia B Rothman-Denes 1, Joanne E Hesse 1, Wolfgang Epstein 1
PMCID: PMC285362  PMID: 4351384

Abstract

Studies of levels of galactokinase in Escherichia coli with mutations affecting synthesis of, or response to, cyclic adenosine 3′,5′-monophosphate show that this nucleotide does not play a major role in expression of the galactose operon, causing at most a twofold stimulation. The discrepancy between our in vivo results and the marked stimulation by cyclic adenosine 3′,5′-monophosphate in in vitro systems indicates that current cell-free systems lack a factor which allows efficient expression of the galactose operon even in the absence of cyclic adenosine 3′,5′-monophosphate or of the binding protein for this nucleotide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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