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. 1973 Nov;116(2):823–831. doi: 10.1128/jb.116.2.823-831.1973

Development of Ultraviolet Resistance in Sporulating Bacillus cereus T

G R Germaine a,1, Elizabeth Coggiola a, W G Murrell a
PMCID: PMC285452  PMID: 4200858

Abstract

Ultraviolet (UV) resistance patterns of sporulating Bacillus cereus were determined. Six different categories of UV resistance were discernible as the organism progressed from exponential growth to the free, mature spore. Light microscope observations allowed the assignment of certain sporulation stages to specific UV resistance categories. Marked changes in UV resistance were associated with stages III, mid-IV, and post-IV of sporulation. Dipicolinic acid was shown to sensitize forespores to UV radiation. Mechanisms invoked to explain the different UV categories are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aronson A. I., Fitz-James P. C. Biosynthesis of bacterial spore coats. J Mol Biol. 1968 Apr 14;33(1):199–212. doi: 10.1016/0022-2836(68)90288-x. [DOI] [PubMed] [Google Scholar]
  2. Donnellan J. E., Jr, Stafford R. S. The ultraviolet photochemistry and photobiology of vegetative cells and spores of Bacillus megaterium. Biophys J. 1968 Jan;8(1):17–28. doi: 10.1016/S0006-3495(68)86471-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fréhel C., Ryter A. Réversibilité de la sporulation chez B. subtilis. Ann Inst Pasteur (Paris) 1969 Sep;117(3):297–311. [PubMed] [Google Scholar]
  4. Germaine G. R., Murrell W. G. Effect of dipicolinic acid on the ultraviolet radiation resistance of Bacillus cereus spores. Photochem Photobiol. 1973 Mar;17(3):145–153. doi: 10.1111/j.1751-1097.1973.tb06344.x. [DOI] [PubMed] [Google Scholar]
  5. Gordon R. A., Murrell W. G. Simple method of detecting spore septum formation and synchrony of sporulation. J Bacteriol. 1967 Jan;93(1):495–496. doi: 10.1128/jb.93.1.495-496.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lewis J. C. Determination of dipicolinic acid in bacterial spores by ultraviolet spectrometry of the calcium chelate. Anal Biochem. 1967 May;19(2):327–337. doi: 10.1016/0003-2697(67)90168-6. [DOI] [PubMed] [Google Scholar]
  7. ROMIG W. R., WYSS O. Some effects of ultraviolet radiation of sporulating cultures of Bacillus cereus. J Bacteriol. 1957 Sep;74(3):386–391. doi: 10.1128/jb.74.3.386-391.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. STEWART B. T., HALVORSON H. O. Studies on the spores of aerobic bacteria. I. The occurrence of alanine racemase. J Bacteriol. 1953 Feb;65(2):160–166. doi: 10.1128/jb.65.2.160-166.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. STUY J. H. Studies on the mechanism of radiation in activation of micro-organisms. III. Inactivation of germinating spores of Bacillus cereus. Biochim Biophys Acta. 1956 Nov;22(2):241–246. doi: 10.1016/0006-3002(56)90146-9. [DOI] [PubMed] [Google Scholar]
  10. Wise J., Swanson A., Halvorson H. O. Dipicolinic acid-less mutants of Bacillus cereus. J Bacteriol. 1967 Dec;94(6):2075–2076. doi: 10.1128/jb.94.6.2075-2076.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

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