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. 1974 Feb;117(2):449–455. doi: 10.1128/jb.117.2.449-455.1974

Derepressed Levels of the Isoleucine-Valine and Leucine Enzymes in hisT1504, a Strain of Salmonella typhimurium with Altered Leucine Transfer Ribonucleic Acid

A A Rizzino 1, R S Bresalier 1, M Freundlich 1
PMCID: PMC285533  PMID: 4359646

Abstract

A hisT mutant of Salmonella typhimurium was found to have altered regulation of the isoleucine-valine and leucine enzymes. These enzymes in the hisT strain were derepressed two- to eightfold over those of the parent wild-type strain when grown in minimal medium or under repressing conditions. The amount of tRNALeu and the cellular concentration of charged tRNALeu was about the same in the hisT strain and in the wild type. However, leucyl-tRNA from the mutant was chromatographically different from that of wild type, confirming previous reports that hisT strains have altered tRNALeu. These results suggest strongly that tRNALeu is involved in repression of the isoleucine-valine and leucine enzymes in S. typhimurium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander R. R., Calvo J. M., Freundlich M. Mutants of Salmonella typhimurium with an altered leucyl-transfer ribonucleic acid synthetase. J Bacteriol. 1971 Apr;106(1):213–220. doi: 10.1128/jb.106.1.213-220.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Allaudeen H. S., Yang S. K., Söll D. Leucine tRNA(1) from HisT mutant of Salmonella typhimurium lacks two pseudouridines. FEBS Lett. 1972 Dec 1;28(2):205–208. doi: 10.1016/0014-5793(72)80713-0. [DOI] [PubMed] [Google Scholar]
  3. Arfin S. M., Koziell D. A. Inhibition of growth of Salmonella typhimurium and of threonine deaminase and transaminase B by beta-chloroalanine. J Bacteriol. 1971 Feb;105(2):519–522. doi: 10.1128/jb.105.2.519-522.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Arfin S. M., Umbarger H. E. Purification and properties of the acetohydroxy acid isomeroreductase of Salmonella typhimurium. J Biol Chem. 1969 Mar 10;244(5):1118–1127. [PubMed] [Google Scholar]
  5. Blatt J. M., Umbarger H. E. On the role of isoleucyl-tRNA synthetase in multivalent repression. Biochem Genet. 1972 Apr;6(2):99–118. doi: 10.1007/BF00486395. [DOI] [PubMed] [Google Scholar]
  6. Brenner M., Ames B. N. Histidine regulation in Salmonella typhimurium. IX. Histidine transfer ribonucleic acid of the regulatory mutants. J Biol Chem. 1972 Feb 25;247(4):1080–1088. [PubMed] [Google Scholar]
  7. Böck A., Faiman L. E., Neidhardt F. C. Biochemical and genetic characterization of a mutant of Escherichia coli with a temperature-sensitive valyl ribonucleic acid synthetase. J Bacteriol. 1966 Oct;92(4):1076–1082. doi: 10.1128/jb.92.4.1076-1082.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Calvo J. M., Freundlich M., Umbarger H. E. Regulation of branched-chain amino acid biosynthesis in Salmonella typhimurium: isolation of regulatory mutants. J Bacteriol. 1969 Mar;97(3):1272–1282. doi: 10.1128/jb.97.3.1272-1282.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FREUNDLICH M., BURNS R. O., UMBARGER H. E. Control of isoleucine, valine, and leucine biosynthesis. I. Multivalent repression. Proc Natl Acad Sci U S A. 1962 Oct 15;48:1804–1808. doi: 10.1073/pnas.48.10.1804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Freundlich M., Clarke L. P. Control of isoleucine, valine and leucine biosynthesis. V. Dual effect of alpha-aminobutyric acid on repression and endproduct inhibition in Escherichia coli. Biochim Biophys Acta. 1968 Dec 23;170(2):271–281. doi: 10.1016/0304-4165(68)90007-x. [DOI] [PubMed] [Google Scholar]
  12. Freundlich M., Trela J., Peng W. Evidence that the majority of leucine transfer ribonucleic acid is not involved in repression in Salmonella typhimurium. J Bacteriol. 1971 Nov;108(2):951–953. doi: 10.1128/jb.108.2.951-953.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hatfield G. W., Burns R. O. Specific binding of leucyl transfer RNA to an immature form of L-threonine deaminase: its implications in repression. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1027–1035. doi: 10.1073/pnas.66.4.1027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Iaccarino M., Berg P. Isoleucine auxotrophy as a consequence of a mutationally altered isoleucyl-transfer ribonucleic acid synthetase. J Bacteriol. 1971 Feb;105(2):527–537. doi: 10.1128/jb.105.2.527-537.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kelmers A. D., Heatherly D. E. Columns for rapid chromatographic separation of small amounts of tracer-labeled transfer ribonucleic acids. Anal Biochem. 1971 Dec;44(2):486–495. doi: 10.1016/0003-2697(71)90236-3. [DOI] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. O'Neill J. P., Freundlich M. Effect of cyclopentaneglycine on metabolism in Salmonella typhimurium. J Bacteriol. 1972 Aug;111(2):510–515. doi: 10.1128/jb.111.2.510-515.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Roe B., Marcu K., Dudock B. The isolation and sequence analysis of transfer RNA: the use of plaskon chromatography (RPC-5). Biochim Biophys Acta. 1973 Aug 10;319(1):25–36. doi: 10.1016/0005-2787(73)90037-3. [DOI] [PubMed] [Google Scholar]
  19. Roth J. R., Antón D. N., Hartman P. E. Histidine regulatory mutants in Salmonella typhimurium. I. Isolation and general properties. J Mol Biol. 1966 Dec 28;22(2):305–323. doi: 10.1016/0022-2836(66)90134-3. [DOI] [PubMed] [Google Scholar]
  20. Singer C. E., Smith G. R., Cortese R., Ames B. N. [Mutant tRNA His ineffective in repression and lacking two pseudouridine modifications]. Nat New Biol. 1972 Jul 19;238(81):72–74. doi: 10.1038/newbio238072a0. [DOI] [PubMed] [Google Scholar]
  21. UMBARGER H. E., BROWN B. Threonine deamination in Escherichia coli. II. Evidence for two L-threonine deaminases. J Bacteriol. 1957 Jan;73(1):105–112. doi: 10.1128/jb.73.1.105-112.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Umbarger H. E. Metabolite analogs as genetic and biochemical probes. Adv Genet. 1971;16:119–140. doi: 10.1016/s0065-2660(08)60356-9. [DOI] [PubMed] [Google Scholar]

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