Abstract
The control mutation that results in a concomitant severalfold increase in the activities of γ-aminobutyrate-α-ketoglutarate transaminase (GSST, EC 2.6.1.19) and succinic semialdehyde dehydrogenase (SSDH, EC 1.2.1.16), leading to the acquisition of the ability to utilize γ-aminobutyrate (GABA) as the sole source of nitrogen by Escherichia coli K-12 mutants, was mapped by mating and transduction with P1kc. The locus affected, gabC, is approximately 48% co-transduced with the thyA gene, located at min 55 of the E. coli K-12 chromosome. The structural gene of the first enzyme in the GABA pathway, GSST, was mapped by interrupted mating, using one of the GSST-less mutants, DB742, isolated in this work. The mutated locus, gabT, is situated at about min 73 of the E. coli chromosome, close to the gltC gene. Genetic evidence concerning the sensitivity of the enzymes of the GABA pathway to catabolite repression under different physiological conditions suggests that the two structural genes of the GABA regulon do not constitute one operon.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAR-GUTTMAN H., HALPERN Y. S., FALK R. A METHOD FOR QUANITATIVE DETERMINATION OF THE RATES OF BACTERIAL AUXOTROPH MUTATIONS. Mutat Res. 1964 Oct;106:334–336. doi: 10.1016/0027-5107(64)90012-0. [DOI] [PubMed] [Google Scholar]
- Dover S., Halpern Y. S. Control of the pathway of -aminobutyrate breakdown in Escherichia coli K-12. J Bacteriol. 1972 Apr;110(1):165–170. doi: 10.1128/jb.110.1.165-170.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dover S., Halpern Y. S. Utilization of -aminobutyric acid as the sole carbon and nitrogen source by Escherichia coli K-12 mutants. J Bacteriol. 1972 Feb;109(2):835–843. doi: 10.1128/jb.109.2.835-843.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAYES W. The kinetics of the mating process in Escherichia coli. J Gen Microbiol. 1957 Feb;16(1):97–119. doi: 10.1099/00221287-16-1-97. [DOI] [PubMed] [Google Scholar]
- LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
- LURIA S. E., ADAMS J. N., TING R. C. Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles. Virology. 1960 Nov;12:348–390. doi: 10.1016/0042-6822(60)90161-6. [DOI] [PubMed] [Google Scholar]
- Low B. Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12. J Bacteriol. 1973 Feb;113(2):798–812. doi: 10.1128/jb.113.2.798-812.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAAS R., MAAS W. K. Introduction of a gene from Escherichia coli B into HFR and F-strains of Escherichia coli K-12. Proc Natl Acad Sci U S A. 1962 Nov 15;48:1887–1893. doi: 10.1073/pnas.48.11.1887. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marcus M., Halpern Y. S. The metabolic pathway of glutamate in Escherichia coli K-12. Biochim Biophys Acta. 1969 Apr 1;177(2):314–320. doi: 10.1016/0304-4165(69)90141-x. [DOI] [PubMed] [Google Scholar]
- Silverstone A. E., Arditti R. R., Magasanik B. Catabolite-insensitive revertants of lac promoter mutants. Proc Natl Acad Sci U S A. 1970 Jul;66(3):773–779. doi: 10.1073/pnas.66.3.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Silverstone A. E., Magasanik B., Reznikoff W. S., Miller J. H., Beckwith J. R. Catabolite sensitive site of the lac operon. Nature. 1969 Mar 15;221(5185):1012–1014. doi: 10.1038/2211012b0. [DOI] [PubMed] [Google Scholar]
- Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]