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. 1974 Feb;117(2):775–782. doi: 10.1128/jb.117.2.775-782.1974

Transcription from the Complementary Deoxyribonucleic Acid Strands of Bacillus subtilis During Various Stages of Sporulation

Chikako Sumida-Yasumoto 1, Roy H Doi 1
PMCID: PMC285572  PMID: 4204440

Abstract

The messenger ribonucleic acid (mRNA) pattern of log-phase and sporulating cells of Bacillus subtilis has been analyzed by deoxyribonucleic acid (DNA)-RNA hybrid studies with the complementary-strand fragments of DNA. Approximately 80% of the mRNA of log-phase and sporulating cells from stages I, III, and IV hybridizes with the heavy DNA fragments, and 20% hybridizes with the light DNA fragments. Hybrid competition studies indicated that there was either a greatly reduced rate of transcription or a turn-off of some log-phase genes during the sporulation stages. However, a significant amount of log-phase gene transcription occurred even at late stages of sporulation. Similar studies indicate a significantly increased rate of transcription or a turn-on of sporulation phase genes during the latter stages of sproulation. There is a sequential increase in the amount of sporulation-specific transcription from both complementary-strand fragments of DNA. These results indicate that the RNA polymerase population in sporulating cells can transcribe both log-phase and sporulation-phase genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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