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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 May;66(1):57–64. doi: 10.1073/pnas.66.1.57

Glycosphingolipids of Plasma Membranes of Cultured Cells and an Enveloped Virus (SV5) Grown in These Cells

Hans-Dieter Klenk 1, Purnell W Choppin 1
PMCID: PMC286087  PMID: 4320464

Abstract

Glycosphingolipids of rhesus monkey kidney (MK), bovine kidney (MDBK), and two lines of hamster kidney (BHK21-F and Hak) cells have been compared with those of parainfluenza (SV5) virions grow in these cells. There are qualitative and quantitative differences in the neutral glycolipids and gangliosides found in the various cells. Cells with a high neutral glycolipid content (MK and MDBK) contain little or no gangliosides, and those with a relatively high ganglioside content (BHK21-F and HaK) contain little neutral glycolipid. Glycosphingolipids are found predominantly in the plasma membranes. Neutral glycolipids of the host cell membrane are incorporated into the envelope of SV5 virions, but neither gangliosides nor protein-bound neuraminic acid are found in virions. The absence of neuraminic acid from the virion may be due to the action of viral neuraminidase.

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Selected References

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  1. AMINOFF D. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961 Nov;81:384–392. doi: 10.1042/bj0810384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adams E. P., Gray G. M. The carbohydrate structures of the neutral ceramide glycolipids in kidneys of different mouse strains with special reference to the ceramide dihexosides. Chem Phys Lipids. 1968 Jun;2(2):147–155. doi: 10.1016/0009-3084(68)90018-2. [DOI] [PubMed] [Google Scholar]
  3. Blough H. A., Lawson D. E. The lipids of paramyxoviruses: a comparative study of Sendai and Newcastle disease viruses. Virology. 1968 Oct;36(2):286–292. doi: 10.1016/0042-6822(68)90146-3. [DOI] [PubMed] [Google Scholar]
  4. CHOPPIN P. W. MULTIPLICATION OF A MYXOVIRUS (SV5) WITH MINIMAL CYTOPATHIC EFFECTS AND WITHOUT INTERFERENCE. Virology. 1964 Jun;23:224–233. doi: 10.1016/0042-6822(64)90286-7. [DOI] [PubMed] [Google Scholar]
  5. Caliguiri L. A., Klenk H. D., Choppin P. W. The proteins of the parainfluenza virus SV5. 1. Separation of virion polypeptides by polyacrylamide gel electrophoresis. Virology. 1969 Nov;39(3):460–466. doi: 10.1016/0042-6822(69)90094-4. [DOI] [PubMed] [Google Scholar]
  6. Compans R. W., Holmes K. V., Dales S., Choppin P. W. An electron microscopic study of moderate and virulent virus-cell interactions of the parainfluenza virus SV5. Virology. 1966 Nov;30(3):411–426. doi: 10.1016/0042-6822(66)90119-x. [DOI] [PubMed] [Google Scholar]
  7. DITTMER J. C., LESTER R. L. A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS. J Lipid Res. 1964 Jan;5:126–127. [PubMed] [Google Scholar]
  8. Eto T., Ichikawa Y., Nishimura K., Ando S., Yamakawa T. Chemistry of lipid of the posthemyolytic residue or stroma of erythrocytes. XVI. Occurrence of ceramide pentasaccharide in the membrane of erythrocytes and reticulocytes of rabbit. J Biochem. 1968 Aug;64(2):205–213. doi: 10.1093/oxfordjournals.jbchem.a128881. [DOI] [PubMed] [Google Scholar]
  9. GRANZER E. [Thin layer chromatography of neuraminic acid derivatives]. Hoppe Seylers Z Physiol Chem. 1962 Aug 6;328:277–279. doi: 10.1515/bchm2.1962.328.1.277. [DOI] [PubMed] [Google Scholar]
  10. Gatt R., Berman E. R. A rapid procedure for the estimation of amino sugars on a micro scale. Anal Biochem. 1966 Apr;15(1):167–171. doi: 10.1016/0003-2697(66)90262-4. [DOI] [PubMed] [Google Scholar]
  11. Hakomori S. I., Murakami W. T. Glycolipids of hamster fibroblasts and derived malignant-transformed cell lines. Proc Natl Acad Sci U S A. 1968 Jan;59(1):254–261. doi: 10.1073/pnas.59.1.254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Holland J. J., Kiehn E. D. Influenza virus effects on cell membrane proteins. Science. 1970 Jan 9;167(3915):202–205. doi: 10.1126/science.167.3915.202. [DOI] [PubMed] [Google Scholar]
  13. Holmes K. V., Choppin P. W. On the role of the response of the cell membrane in determining virus virulence. Contrasting effects of the parainfluenza virus SV5 in two cell types. J Exp Med. 1966 Sep 1;124(3):501–520. doi: 10.1084/jem.124.3.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Holmes K. V., Klenk H. D., Choppin P. W. A comparison of immune cytolysis and virus-induced fusion of sensitive and resistant cell types. Proc Soc Exp Biol Med. 1969 Jun;131(2):651–657. doi: 10.3181/00379727-131-33945. [DOI] [PubMed] [Google Scholar]
  15. Isacson P., Koch A. E. Association of host antigens with a parainfluenza virus. Virology. 1965 Oct;27(2):129–138. doi: 10.1016/0042-6822(65)90153-4. [DOI] [PubMed] [Google Scholar]
  16. KLENK E., GIELEN W. [On a 2d hexose-containing ganglioside from human brain]. Hoppe Seylers Z Physiol Chem. 1963 Mar 10;330:218–226. [PubMed] [Google Scholar]
  17. Klenk H. D., Choppin P. W. Chemical composition of the parainfluenza virus SV5. Virology. 1969 Jan;37(1):155–157. doi: 10.1016/0042-6822(69)90321-3. [DOI] [PubMed] [Google Scholar]
  18. Klenk H. D., Choppin P. W. Lipids of plasma membranes of monkey and hamster kidney cells and of parainfluenza virions grown in these cells. Virology. 1969 Jun;38(2):255–268. doi: 10.1016/0042-6822(69)90367-5. [DOI] [PubMed] [Google Scholar]
  19. Laver W. G., Webster R. G. The structure of influenza viruses. IV. Chemical studies of the host antigen. Virology. 1966 Sep;30(1):104–115. doi: 10.1016/s0042-6822(66)81014-0. [DOI] [PubMed] [Google Scholar]
  20. Lee L. T., Howe C., Meyer K., Choi H. U. Quantitative precipitin analysis of influenza virus host antigen and of sulfated mucopolysaccharides of chicken embryonic allantoic fluid. J Immunol. 1969 May;102(5):1144–1155. [PubMed] [Google Scholar]
  21. Mora P. T., Brady R. O., Bradley R. M., McFarland V. W. Gangliosides in DNA virus-transformed and spontaneously transformed tumorigenic mouse cell lines. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1290–1296. doi: 10.1073/pnas.63.4.1290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SUZUKI K. A SIMPLE AND ACCURATE MICROMETHOD FOR QUANTITATIVE DETERMINATION OF GANGLIOSIDE PATTERNS. Life Sci. 1964 Nov;3:1227–1233. doi: 10.1016/0024-3205(64)90040-2. [DOI] [PubMed] [Google Scholar]

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