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. 1989 Jan;86(1):147–151. doi: 10.1073/pnas.86.1.147

Evidence for selenocysteine coordination to the active site nickel in the [NiFeSe]hydrogenases from Desulfovibrio baculatus.

M K Eidsness 1, R A Scott 1, B C Prickril 1, D V DerVartanian 1, J Legall 1, I Moura 1, J J Moura 1, H D Peck Jr 1
PMCID: PMC286421  PMID: 2521386

Abstract

Ni and Se x-ray absorption spectroscopic studies of the [NiFeSe]hydrogenases from Desulfovibrio baculatus are described. The Ni site geometry is pseudo-octahedral with a coordinating ligand composition of 3-4 (N,O) at 2.06 A, 1-2 (S,Cl) at 2.17 A, and 1 Se at 2.44 A. The Se coordination environment consists of 1 C at 2.0 A and a heavy scatterer M (M = Ni or Fe) at approximately 2.4 A. These results are interpreted in terms of a selenocysteine residue coordinated to the Ni site. The possible role of the Ni-Se site in the catalytic activation of H2 is discussed.

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Selected References

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