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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1989 Jan;86(2):577–581. doi: 10.1073/pnas.86.2.577

Construction of functional artificial minichromosomes in the fission yeast Schizosaccharomyces pombe.

K M Hahnenberger 1, M P Baum 1, C M Polizzi 1, J Carbon 1, L Clarke 1
PMCID: PMC286515  PMID: 2643117

Abstract

The centromere DNAs from chromosomes I and III of Schizosaccharomyces pombe have been cloned in an artificial chromosome vector in both budding and fission yeasts. In S. pombe, synthetic linear and circular minichromosomes containing an intact centromere are stable mitotically and behave as independent genetic linkage groups that segregate properly through meiosis. These experiments present a general strategy for the isolation of centromeres from other organisms.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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