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. 1989 May;86(10):3599–3603. doi: 10.1073/pnas.86.10.3599

Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase.

J Colicelli 1, C Birchmeier 1, T Michaeli 1, K O'Neill 1, M Riggs 1, M Wigler 1
PMCID: PMC287185  PMID: 2542941

Abstract

A rat brain cDNA library has been constructed in a Saccharomyces cerevisiae expression vector and used to isolate genes that can function in yeast to suppress the phenotypic effects of RAS2val19, a mutant form of the RAS2 gene analogous to an oncogenic mutant of the human HRAS gene. One cDNA, DPD, was cloned and its genetic and biochemical properties were characterized. A DPD product would share 80% amino acid sequence identity with the Drosophila melanogaster dunce-encoded protein over an extended region. We have shown that the DPD protein is a high-affinity cAMP-specific phosphodiesterase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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