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. 1989 May;86(10):3609–3613. doi: 10.1073/pnas.86.10.3609

Aspartyl beta-hydroxylase: in vitro hydroxylation of a synthetic peptide based on the structure of the first growth factor-like domain of human factor IX.

R S Gronke 1, W J VanDusen 1, V M Garsky 1, J W Jacobs 1, M K Sardana 1, A M Stern 1, P A Friedman 1
PMCID: PMC287187  PMID: 2726737

Abstract

beta-Hydroxylation of aspartic acid is a post-translational modification that occurs in several vitamin K-dependent coagulation proteins. By use of a synthetic substrate comprised of the first epidermal growth factor-like domain in human factor IX and either mouse L-cell extracts or rat liver microsomes as the source of enzyme, in vitro aspartyl beta-hydroxylation was accomplished. Aspartyl beta-hydroxylase appears to require the same cofactors as known alpha-ketoglutarate-dependent dioxygenases. The hydroxylation reaction proceeds with the same stereospecificity and occurs only at the aspartate corresponding to the position seen in vivo. Further purification and characterization of this enzymatic activity should now be possible.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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