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. 1989 May;86(10):3919–3923. doi: 10.1073/pnas.86.10.3919

Activity of ion channels during volume regulation by clonal N1E115 neuroblastoma cells.

L C Falke 1, S Misler 1
PMCID: PMC287253  PMID: 2471194

Abstract

When exposed to a hypotonic bathing solution, clonal N1E115 neuroblastoma cells initially swell and then undergo a regulatory volume decrease (RVD). Using cell-attached patch-clamp recording, we have found that the activity of a stretch-sensitive, nonselective cation [C+(SA)] channel increases shortly after the onset of osmotically induced cell swelling; this depolarizes the cells as much as 30 mV. Shortly thereafter, and roughly coincident with the onset of RVD, two types of voltage-dependent channels open at the new resting potential; these are (i) a delayed-rectifier type K+ [K+(DR)] channel and (ii) a large-conductance anion channel. We suggest that opening of the C+(SA) channel may contribute to the volume "sensor" mechanism, while the depolarization-induced opening of the K+(DR) and anion channels may constitute a significant K+ salt exit pathway, operating in RVD.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Christensen O. Mediation of cell volume regulation by Ca2+ influx through stretch-activated channels. Nature. 1987 Nov 5;330(6143):66–68. doi: 10.1038/330066a0. [DOI] [PubMed] [Google Scholar]
  2. Cooper K. E., Tang J. M., Rae J. L., Eisenberg R. S. A cation channel in frog lens epithelia responsive to pressure and calcium. J Membr Biol. 1986;93(3):259–269. doi: 10.1007/BF01871180. [DOI] [PubMed] [Google Scholar]
  3. Falke L. C., Edwards K. L., Pickard B. G., Misler S. A stretch-activated anion channel in tobacco protoplasts. FEBS Lett. 1988 Sep 12;237(1-2):141–144. doi: 10.1016/0014-5793(88)80188-1. [DOI] [PubMed] [Google Scholar]
  4. Gray P. T., Bevan S., Ritchie J. M. High conductance anion-selective channels in rat cultured Schwann cells. Proc R Soc Lond B Biol Sci. 1984 Jun 22;221(1225):395–409. doi: 10.1098/rspb.1984.0041. [DOI] [PubMed] [Google Scholar]
  5. Guharay F., Sachs F. Stretch-activated single ion channel currents in tissue-cultured embryonic chick skeletal muscle. J Physiol. 1984 Jul;352:685–701. doi: 10.1113/jphysiol.1984.sp015317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kirber M. T., Walsh J. V., Jr, Singer J. J. Stretch-activated ion channels in smooth muscle: a mechanism for the initiation of stretch-induced contraction. Pflugers Arch. 1988 Sep;412(4):339–345. doi: 10.1007/BF01907549. [DOI] [PubMed] [Google Scholar]
  7. Kolb H. A., Brown C. D., Murer H. Identification of a voltage-dependent anion channel in the apical membrane of a Cl(-)-secretory epithelium (MDCK). Pflugers Arch. 1985 Mar;403(3):262–265. doi: 10.1007/BF00583597. [DOI] [PubMed] [Google Scholar]
  8. Lansman J. B., Hallam T. J., Rink T. J. Single stretch-activated ion channels in vascular endothelial cells as mechanotransducers? 1987 Feb 26-Mar 4Nature. 325(6107):811–813. doi: 10.1038/325811a0. [DOI] [PubMed] [Google Scholar]
  9. Lee S. C., Price M., Prystowsky M. B., Deutsch C. Volume response of quiescent and interleukin 2-stimulated T-lymphocytes to hypotonicity. Am J Physiol. 1988 Feb;254(2 Pt 1):C286–C296. doi: 10.1152/ajpcell.1988.254.2.C286. [DOI] [PubMed] [Google Scholar]
  10. Matteson D. R., Deutsch C. K channels in T lymphocytes: a patch clamp study using monoclonal antibody adhesion. Nature. 1984 Feb 2;307(5950):468–471. doi: 10.1038/307468a0. [DOI] [PubMed] [Google Scholar]
  11. Misler S., Falke L. C., Gillis K., McDaniel M. L. A metabolite-regulated potassium channel in rat pancreatic B cells. Proc Natl Acad Sci U S A. 1986 Sep;83(18):7119–7123. doi: 10.1073/pnas.83.18.7119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Quandt F. N. Three kinetically distinct potassium channels in mouse neuroblastoma cells. J Physiol. 1988 Jan;395:401–418. doi: 10.1113/jphysiol.1988.sp016926. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sackin H. A stretch-activated K+ channel sensitive to cell volume. Proc Natl Acad Sci U S A. 1989 Mar;86(5):1731–1735. doi: 10.1073/pnas.86.5.1731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Soejima M., Kokubun S. Single anion-selective channel and its ion selectivity in the vascular smooth muscle cell. Pflugers Arch. 1988 Mar;411(3):304–311. doi: 10.1007/BF00585119. [DOI] [PubMed] [Google Scholar]
  15. Woll K. H., Leibowitz M. D., Neumcke B., Hille B. A high-conductance anion channel in adult amphibian skeletal muscle. Pflugers Arch. 1987 Dec;410(6):632–640. doi: 10.1007/BF00581324. [DOI] [PubMed] [Google Scholar]

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