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. 1989 Jun;86(12):4425–4429. doi: 10.1073/pnas.86.12.4425

Mismatch repair involving localized DNA synthesis in extracts of Xenopus eggs.

P Brooks 1, C Dohet 1, G Almouzni 1, M Méchali 1, M Radman 1
PMCID: PMC287282  PMID: 2734295

Abstract

Repair of heteroduplex DNA containing G.T or A.C mismatches or containing two tandem unpaired bases occurred in vitro with Xenopus egg extracts as detected by a physical assay. The repair was accompanied by a mismatch-stimulated and mismatch-localized DNA synthesis. Repaired molecules, separated from unrepaired molecules, showed a 20- to 100-fold increase in DNA synthesis in the region of the mismatch compared to regions distant from the mismatch. The remaining unrepaired heteroduplex DNA included molecules that also displayed mismatch-stimulated DNA synthesis in the mismatch-proximal regions. These may represent intermediates in the repair process. The patterns of DNA synthesis suggest that repair begins at some distance from the mismatch and that as much as 1 kilobase or more can be involved in the mismatch-stimulated synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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