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. 1980 Jan;33(1):123–128. doi: 10.1128/jvi.33.1.123-128.1980

C3H/HeN mammary tumor-bearing mice develop type-specific neutralizing antibodies and group-specific precipitating antibodies for the mouse mammary tumor virus.

R Massey, L O Arthur, C W Long, G Schochetman
PMCID: PMC288530  PMID: 6154146

Abstract

The development of mouse mammary tumor virus (MMTV)-neutralizing antibodies in various strains of mice was measured by their ability to neutralize the focus-forming capacity of a Kirsten sarcoma virus (C3H MMTV) pseudotype containing the MMTV envelope glycoprotein gp52. C3H/HeN, but not GR/N and RIII, mammary tumor-bearing mice were found to develop neutralizing antibodies to this pseudotype. In addition, non-tumor-bearing C3H/HeN, GR/N, RIII, NIH Swiss, C57BL/6, and BALB/c mice and 13 feral mice were also negative for neutralizing antibodies. The neutralization was immunoglobulin G mediated, and the antibodies of C3H/HeN mammary tumor-bearing mice were type specific and capable of distinguishing C3H and GR/N MMTVs from RIII and C3H/HeNf MMTVs. Precipitating antibodies were detected in sera from RIII and GR/N tumor-bearing mice, GR/N non-tumor-bearing mice, and six of the feral mice, although these same sera did not neutralize the Kirsten sarcoma virus (C3H MMTV) pseudotype. The results of this study and of a previous study demonstrate that C3H/HeN mammary tumor-bearing mice develop three functionally distinct antibody populations: (i) group-specific virus-precipitating antibodies; (ii) type-specific virus-neutralizing antibodies; and (iii) type-specific cytotoxic antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arthur L. O., Bauer R. F., Orme L. S., Fine D. L. Coexistence of the mouse mammary tumor virus (MMTV) major glycoprotein and natural antibodies to MMTV in sera of mammary tumor-bearing mice. Virology. 1978 Jun 15;87(2):266–275. doi: 10.1016/0042-6822(78)90132-0. [DOI] [PubMed] [Google Scholar]
  2. Arthur L. O., Fine D. L. Immunological characterization of mouse mammary tumor virus p10 and its presence in mammary tumors and sera of tumor-bearing mice. J Virol. 1979 Apr;30(1):148–156. doi: 10.1128/jvi.30.1.148-156.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Arthur L. O., Fine D. L. Naturally occurring humoral immunity to murine mammary tumor virus (MuMTV) and MuMTV GP52 in mice with low mammary tumor incidence. Int J Cancer. 1978 Dec;22(6):734–740. doi: 10.1002/ijc.2910220616. [DOI] [PubMed] [Google Scholar]
  4. Arthur L. O., Lovinger G. G., Schochetman G. Establishment of a C3Hf mammary tumor cell line expressing endogenous mouse mammary tumor virus: antigenic and genetic relationships of this virus with highly oncogenic mouse mammary tumor viruses. J Virol. 1979 Dec;32(3):852–859. doi: 10.1128/jvi.32.3.852-859.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Blair P. B. Immunology of the mouse mammary tumor virus (MTV): neutralization of MTV by mouse antiserum. Cancer Res. 1968 Jan;28(1):148–149. [PubMed] [Google Scholar]
  6. Blair P. B., Kavrin D. H., Dezfulian M., Weiss D. W. Immunology of the mouse mammary tumor virus (MTV) : identification in vitro of mouse antibodies against MTV. Cancer Res. 1966 Apr;26(4):647–651. [PubMed] [Google Scholar]
  7. DULBECCO R., VOGT M., STRICKLAND A. G. A study of the basic aspects of neutralization of two animal viruses, western equine encephalitis virus and poliomyelitis virus. Virology. 1956 Apr;2(2):162–205. doi: 10.1016/0042-6822(56)90017-4. [DOI] [PubMed] [Google Scholar]
  8. Fink M. A., Feller W. F., Sibal L. R. Methods for detection of antibody to the mammary tumor virus. J Natl Cancer Inst. 1968 Dec;41(6):1395–1400. [PubMed] [Google Scholar]
  9. Holmes E. C., Morton D. L. Detection of antibodies against the mammary tumor virus with the antiglobulin consumption test. J Natl Cancer Inst. 1969 May;42(5):733–738. [PubMed] [Google Scholar]
  10. Ihle J. N., Arthur L. O., Fine D. L. Autogenous immunity to mouse mammary tumor virus in mouse strains of high and low mammary tumor incidence. Cancer Res. 1976 Aug;36(8):2840–2844. [PubMed] [Google Scholar]
  11. Nowinski R. C., Lostrom M. E., Tam M. R., Stone M. R., Burnette W. N. The isolation of hybrid cell lines producing monoclonal antibodies against the p15(E) protein of ecotropic murine leukemia viruses. Virology. 1979 Feb;93(1):111–126. doi: 10.1016/0042-6822(79)90280-0. [DOI] [PubMed] [Google Scholar]
  12. Nowinski R. C., Old L. J., Moore D. H., Geering G., Boyse E. A. A soluble antigen of the mammary tumor virus. Virology. 1967 Jan;31(1):1–14. doi: 10.1016/0042-6822(67)90002-5. [DOI] [PubMed] [Google Scholar]
  13. Schochetman G., Arthur L. O., Long C. W., Massey R. J. Mice with spontaneous mammary tumors develop type-specific neutralizing and cytotoxic antibodies against the mouse mammary tumor virus envelope protein gp52. J Virol. 1979 Oct;32(1):131–139. doi: 10.1128/jvi.32.1.131-139.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schochetman G., Long C., Massey R. Generation of a mouse mammary tumor virus (MMTV) pseudotype of Kirsten sarcoma virus and restriction of MMTV gag expression in heterologous infected cells. Virology. 1979 Sep;97(2):342–353. doi: 10.1016/0042-6822(79)90345-3. [DOI] [PubMed] [Google Scholar]
  15. Závada J., Dickson C., Weiss R. Pseudotypes of vesicular stomatitis virus with envelope antigens provided by murine mammary tumor virus. Virology. 1977 Oct 1;82(1):221–231. doi: 10.1016/0042-6822(77)90045-9. [DOI] [PubMed] [Google Scholar]

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