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. 1980 Jul;35(1):259–264. doi: 10.1128/jvi.35.1.259-264.1980

Three independent isolates of feline sarcoma virus code for three distinct gag-x polyproteins.

S K Ruscetti, L P Turek, C J Sherr
PMCID: PMC288804  PMID: 6251274

Abstract

Cells nonproductively transformed by the Snyder-Theilen, Gardner-Arnstein, and McDonough strains of feline sarcoma virus synthesize gag-x polyproteins of 78,000, 100,000, and 180,000 daltons, respectively. These feline sarcoma virus-coded products were precipitated by antisera to polypeptides encoded by the gag gene of feline leukemia virus and by rat antisera raised to feline sarcoma virus-transformed rat tumor cells. Precipitation with rat antisera absorbed with feline leukemia virus showed that the x-portions of the three gag-x proteins were each antigenically distinct, suggesting that the src genes of the three independent isolates are not identical. Anti-x sera did not precipitate products from radiolabeled cat lymphoid tumor cells (FL74) and therefore lacked reactivity to the feline leukemia virus-induced tumor-specific antigen, FOCMA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barbacid M., Stephenson J. R., Aaronson S. A. gag Gene of mammalian type-C RNA tumour viruses. Nature. 1976 Aug 12;262(5569):554–559. doi: 10.1038/262554a0. [DOI] [PubMed] [Google Scholar]
  2. Beemon K., Hunter T., Sefton B. M. Polymorphism of avian sarcoma virus src proteins. J Virol. 1979 Apr;30(1):190–200. doi: 10.1128/jvi.30.1.190-200.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Essex M. Horizontally and vertically transmitted oncornaviruses of cats. Adv Cancer Res. 1975;21:175–248. doi: 10.1016/s0065-230x(08)60973-2. [DOI] [PubMed] [Google Scholar]
  4. Frankel A. E., Gilbert J. H., Porzig K. J., Scolnick E. M., Aaronson S. A. Nature and distribution of feline sarcoma virus nucleotide sequences. J Virol. 1979 Jun;30(3):821–827. doi: 10.1128/jvi.30.3.821-827.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gardner M. B., Rongey R. W., Arnstein P., Estes J. D., Sarma P., Huebner R. J., Rickard C. G. Experimental transmission of feline fibrosarcoma to cats and dogs. Nature. 1970 May 30;226(5248):807–809. doi: 10.1038/226807a0. [DOI] [PubMed] [Google Scholar]
  6. Hardy W. D., Jr, Zuckerman E. E., MacEwen E. G., Hayes A. A., Essex M. A feline leukaemia virus- and sarcoma virus-induced tumour-specific antigen. Nature. 1977 Nov 17;270(5634):249–251. doi: 10.1038/270249a0. [DOI] [PubMed] [Google Scholar]
  7. Henderson I. C., Lieber M. M., Todaro G. J. Mink cell line Mv 1 Lu (CCL 64). Focus formation and the generation of "nonproducer" transformed cell lines with murine and feline sarcoma viruses. Virology. 1974 Jul;60(1):282–287. doi: 10.1016/0042-6822(74)90386-9. [DOI] [PubMed] [Google Scholar]
  8. Khan A. S., Deobagkar D. N., Stephenson J. R. Isolation and characterization of a feline sarcoma virus-coded precursor polyprotein. Competition immunoassay for nonstructural components. J Biol Chem. 1978 Dec 25;253(24):8894–8901. [PubMed] [Google Scholar]
  9. Khan A. S., Stephenson J. R. Feline leukemia virus: biochemical and immunological characterization of gag gene-coded structural proteins. J Virol. 1977 Sep;23(3):599–607. doi: 10.1128/jvi.23.3.599-607.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McDonough S. K., Larsen S., Brodey R. S., Stock N. D., Hardy W. D., Jr A transmissible feline fibrosarcoma of viral origin. Cancer Res. 1971 Jul;31(7):953–956. [PubMed] [Google Scholar]
  11. Porzig K. J., Barbacid M., Aaronson S. A. Biological properties and translational products of three independent isolates of feline sarcoma virus. Virology. 1979 Jan 15;92(1):91–107. doi: 10.1016/0042-6822(79)90217-4. [DOI] [PubMed] [Google Scholar]
  12. Rickard C. G., Post J. E., Noronha F., Barr L. M. A transmissible virus-induced lymphocytic leukemia of the cat. J Natl Cancer Inst. 1969 Jun;42(6):987–1014. [PubMed] [Google Scholar]
  13. Robbins K. C., Barbacid M., Porzig K. J., Aaronson S. A. Involvement of different exogenous feline leukemia virus subgroups in the generation of independent feline sarcoma virus isolates. Virology. 1979 Aug;97(1):1–11. doi: 10.1016/0042-6822(79)90367-2. [DOI] [PubMed] [Google Scholar]
  14. Ruscetti S. K., Linemeyer D., Feild J., Troxler D., Scolnick E. M. Characterization of a protein found in cells infected with the spleen focus-forming virus that shares immunological cross-reactivity with the gp70 found in mink cell focus-inducing virus particles. J Virol. 1979 Jun;30(3):787–798. doi: 10.1128/jvi.30.3.787-798.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sarma P. S., Sharar A. L., McDonough S. The SM strain of feline sarcoma virus. Biologic and antigenic characterization of virus. Proc Soc Exp Biol Med. 1972 Sep;140(4):1365–1368. doi: 10.3181/00379727-140-36675. [DOI] [PubMed] [Google Scholar]
  16. Scolnick E. M., Parks W. P., Livingston D. M. Radioimmunoassay of mammalian type C viral proteins. I. Species specific reactions of murine and feline viruses. J Immunol. 1972 Sep;109(3):570–577. [PubMed] [Google Scholar]
  17. Sherr C. J., Fedele L. A., Donner L., Turek L. P. Restriction endonuclease mapping of unintegrated proviral DNA of Snyder-Theilen feline sarcoma virus: localization of sarcoma-specific sequences. J Virol. 1979 Dec;32(3):860–875. doi: 10.1128/jvi.32.3.860-875.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sherr C. J., Fedele L. A., Oskarsson M., Maizel J., Vande Woude G. Molecular cloning of Snyder-Theilen feline leukemia and sarcoma viruses: comparative studies of feline sarcoma virus with its natural helper virus and with Moloney murine sarcoma virus. J Virol. 1980 Apr;34(1):200–212. doi: 10.1128/jvi.34.1.200-212.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sherr C. J., Sen A., Todaro G. J., Sliski A., Essex M. Pseudotypes of feline sarcoma virus contain an 85,000-dalton protein with feline oncornavirus-associated cell membrane antigen (FOCMA) activity. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1505–1509. doi: 10.1073/pnas.75.3.1505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sherr C. J., Todaro G. J., Sliski A., Essex M. Characterization of a feline sarcoma virus-coded antigen (FOCMA-S) by radioimmunoassay. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4489–4493. doi: 10.1073/pnas.75.9.4489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shih T. Y., Weeks M. O., Young H. A., Scolnick E. M. p21 of Kirsten murine sarcoma virus is thermolabile in a viral mutant temperature sensitive for the maintenance of transformation. J Virol. 1979 Aug;31(2):546–546. doi: 10.1128/jvi.31.2.546-546.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sliski A. H., Essex M., Meyer C., Todaro G. Feline oncornavirus-associated cell membrane antigen: expression in transformed nonproducer mink cells. Science. 1977 Jun 17;196(4296):1336–1339. doi: 10.1126/science.194310. [DOI] [PubMed] [Google Scholar]
  23. Sliski A. H., Essex M. Sarcoma virus-induced transformation specific antigen: presence of antibodies in cats that were naturally exposed to leukemia virus. Virology. 1979 Jun;95(2):581–586. doi: 10.1016/0042-6822(79)90512-9. [DOI] [PubMed] [Google Scholar]
  24. Snyder H. W., Jr, Hardy W. D., Jr, Zuckerman E. E., Fleissner E. Characterisation of a tumour-specific antigen on the surface of feline lymphosarcoma cells. Nature. 1978 Oct 19;275(5681):656–658. doi: 10.1038/275656a0. [DOI] [PubMed] [Google Scholar]
  25. Snyder S. P., Theilen G. H. Transmissible feline fibrosarcoma. Nature. 1969 Mar 15;221(5185):1074–1075. doi: 10.1038/2211074a0. [DOI] [PubMed] [Google Scholar]
  26. Stephenson J. R., Khan A. S., Sliski A. H., Essex M. Feline oncornavirus-associated cell membrane antigen: evidence for an immunologically crossreactive feline sarcoma virus-coded protein. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5608–5612. doi: 10.1073/pnas.74.12.5608. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Theilen G. H., Kawakami T. G., Rush J. D., Munn R. J. Replication of cat leukemia virus in cell suspension cultures. Nature. 1969 May 10;222(5193):589–590. doi: 10.1038/222589b0. [DOI] [PubMed] [Google Scholar]

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