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. 1980 Aug;35(2):498–504. doi: 10.1128/jvi.35.2.498-504.1980

Characterization of JC papovavirus adapted to growth in human embryonic kidney cells.

T Miyamura, K Yoshiike, K K Takemoto
PMCID: PMC288835  PMID: 6255188

Abstract

Human papovavirus JC virus was adapted to growth in human embryonic kidney (HEK) cells. After eight passages, the HEK-adapted JC virus produced high virus yields and was capable of forming plaques in HEK monolayer cultures. Eleven plaque-purified stocks were prepared and characterized. Biologically, the plaque-purified virus induced tumor and viral antigens in HEK cells earlier and in a higher percentage of cells than uncloned virus. Cytopathic changes were also evident sooner and were more extensive. The DNA from uncloned as well as plaque-purified isolates was analyzed by restriction endonuclease cleavage followed by gel electrophoresis. The DNA from uncloned HEK-adapted virus was heterogeneous. Plaque-purified virus isolates yielded DNA which, although much less heterogeneous than the uncloned stock, still consisted of two or more species of viral DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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