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. 1980 Sep;35(3):629–636. doi: 10.1128/jvi.35.3.629-636.1980

Sites of synthesis of viral proteins in avian sarcoma virus-infected chicken cells.

A F Purchio, S Jovanovich, R L Erikson
PMCID: PMC288856  PMID: 6252329

Abstract

We determined the sites of synthesis of avian sarcoma virus-specific proteins in infected chicken cells by immunoprecipitation of the products synthesized in vitro by free and membrane-bound polyribosomes; 85% of Pr76, the precursor of the viral internal structural proteins (group-specific antigens), was synthesized on free polyribosomes, and 15% was synthesized on membrane-bound polyribosomes. Pr92, the lycosylated precursor of the viral glycoproteins (gp85 and gp35), was synthesized exclusively on membrane-bound polyribomes, which is consistent with its role as a membrane protein. When we investigated the site of synthesis of pp60src, the product of the avian sarcoma virus src gene, we found that 90% was synthesized on free polyribosomes, whereas 10% was detected on membrane-bound polyribosomes. The implications of these results with respect to the subcellular location of pp60src are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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