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. 1986 Nov;60(2):793–796. doi: 10.1128/jvi.60.2.793-796.1986

Production of guanidine-resistant and -dependent poliovirus mutants from cloned cDNA: mutations in polypeptide 2C are directly responsible for altered guanidine sensitivity.

S E Pincus, E Wimmer
PMCID: PMC288960  PMID: 3022012

Abstract

cDNA fragments representing the region in polypeptide 2C containing mutations in a guanidine-resistant or -dependent mutant were cloned into the wild-type background of an infectious clone. Transfection of COS-1 cells with these plasmids yielded viruses that were either completely resistant to 2.0 mM guanidine hydrochloride or dependent on this concentration of drug for growth.

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Selected References

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