Abstract
A defined medium for Aquaspirillum serpens VHL allows the replacement of the complex media now in use. It was developed by batch culture methods but supports growth in continuous culture. A basal salts medium supplemented with L-aspartic acid, L-alanine, and L-glutamic acid provided the best growth (turbidity), as long as ammonium chloride was omitted. Ammonium chloride caused either a lag or a reduction or a complete inhibition of the growth of A. serpens VHL on the above amino acids and other organic supplements depending on the combination used. Ammonium sulfate and ammonium hydroxide with L-glutamic acid allowed growth, but the lag period was increased in shake flask cultures. Vitamins, cysteine hydrochloride, and carbon dioxide had no effect on the growth rate. Viability (less than 50%) was inadequate to maintain continuous culture with L-glutamic acid as the sole source of carbon and nitrogen. Combinations of amino and carboxylic acids were then tested and, of these, L-glutamic acid (1 g/liter) and L-histidine (75 mg/liter) without ammonium chloride in the basal salts medium supported growth in batch and continuous culture. L-Glutamic acid was the limiting substrate for growth.
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Selected References
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- Crothers S. F., Robinson J. Changes in the permeability of Escherichia coli during parasitization by Bdellovibrio bacteriovorus. Can J Microbiol. 1971 May;17(5):689–697. doi: 10.1139/m71-111. [DOI] [PubMed] [Google Scholar]
- Dias F. F., Dondero N. A., Finstein M. S. Attached growth of Sphaerotilus and mixed populations in a continuous-flow apparatus. Appl Microbiol. 1968 Aug;16(8):1191–1199. doi: 10.1128/am.16.8.1191-1199.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kolenbrander P. E., Ensign J. C. Isolation and chemical structure of the peptidoglycan of Spirillum serpens cell walls. J Bacteriol. 1968 Jan;95(1):201–210. doi: 10.1128/jb.95.1.201-210.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Krieg N. R. Biology of the chemoheterotrophic spirilla. Bacteriol Rev. 1976 Mar;40(1):55–115. doi: 10.1128/br.40.1.55-115.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kropinski A. M. A chemically defined medium for Aquaspirillum aquaticum ATCC 11330. Can J Microbiol. 1975 Nov;21(11):1886–1889. doi: 10.1139/m75-274. [DOI] [PubMed] [Google Scholar]
- STOKES J. L. Studies on the filamentous sheathed iron bacterium Sphaerotilus natans. J Bacteriol. 1954 Mar;67(3):278–291. doi: 10.1128/jb.67.3.278-291.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]