Oxidation of multiple methionine residues impairs rapid sodium channel inactivation

Abstract

Reactive oxygen species (ROS) readily oxidize the sulfur-containing amino acids cysteine and methionine (Met). The impact of Met oxidation on the fast inactivation of the skeletal muscle sodium channel Na_V1.4 expressed in human embryonic kidney cells was studied by applying the Met-preferring oxidant chloramine-T (ChT) or by irradiating the ROS-producing dye Lucifer Yellow in the patch pipettes. Both interventions dramatically slowed down inactivation of the sodium channels. Replacement of Met in the Ile-Phe-Met inactivation motif with Leu (M1305L) strongly attenuated the oxidizing effect on inactivation but did not eliminate it completely. Mutagenesis of conserved Met residues in the intracellular linkers connecting the membrane-spanning segments of the channel (M1469L and M1470L) also markedly diminished the oxidation sensitivity of the channel, while that of other conserved Met residues (442, 1139, 1154, 1316) were without any noticeable effect. The results of mutagenesis of results, assays of other Na_V channel isoforms (Na_V1.2, Na_V1.5, Na_V1.7) and the kinetics of the oxidation-induced removal of inactivation collectively indicate that multiple Met target residues need to be oxidized to completely impair inactivation. This arrangement using multiple Met residues confers a finely graded oxidative modulation of Na_V channels and allows organisms to adapt to a variety of oxidative stress conditions, such as ischemic reperfusion.

INTRODUCTION

Excess reactive oxygen species (ROS), as found in oxidation stress situations, affect lipids, nucleic acids and proteins in cells. In proteins the sulphur-containing amino acids cysteine (Cys) and methionine (Met) are readily oxidized by ROS [1]. Oxidation of Met generates the two Met sulfoxide (MetO) epimers Met-S-O and Met-R-O, which are reduced by the methionine sulfoxide reductases (MSRs) A and B, respectively [2]. Oxidation and reduction of methionines may serve different functions [3]: (1) Intracellular ROS can be scavenged by reversible oxidation of Met residues that are not critical for the function of a protein. Such residues may prevent other critical Met residues from becoming oxidized, as was postulated for the E. coli glutamine synthetase enzyme complex [4]. (2) MSRs may reduce oxidized Met residues that are critical for protein function, thus serving a role as repair enzymes. For example, oxidative loss of calmodulin functions, such as activation of plasma membrane Ca²⁺-ATPase, may be restored by MSRs [5]. (3) Reversible Met oxidation may regulate specific oxidation-sensitive processes. Coexpression of Drosophila Shaker C/B potassium channels in Xenopus oocytes with MSRA or MSRB protects fast inactivation of the channel against oxidation, an effect that could be attributed to a Met residue in the N-terminal ball domain, which is responsible for fast inactivation [6–8].

Several lines of evidence argue that oxidative modification of voltage-gated sodium channels (Na_V channels) with pathophysiological consequences also occurs (e.g., [9–12]) but the underlying molecular mechanisms remain elusive. Na_V channels rapidly open upon membrane depolarization to allow Na⁺ influx but the influx is transient because the channels inactivate quickly. In this inactivation process, a hydrophobic triad consisting of Ile-Phe-Met (IFM) in the linker between domains 3 and 4 (D3–D4) of all Na_v channels interacts with moieties on the channels’ inner pore entries (e.g., [13,14]). Since MetO is more hydrophilic than Met [15], the hydrophobic interaction between the linker and its receptor on the channel may be disturbed if MetO is present. In fact, several studies using oxidants, such as ChT and H₂O₂, indicated that oxidation of Met may impair fast inactivation in both neuronal and muscle Na_v channels [16–19]. Similar effects are evoked by irradiation of HEK 293 cells expressing the human isoforms of Na_V1.4 or Na_V1.5 with UV-A (320–380 nm wavelength) light, which triggers the production of intracellular ROS [20]. However, a mutant of the rat Na_V1.4 channel with the inactivating IFM motif mutated to IFI remained sensitive to both, UV-A and H₂O₂ exposure [20], thus suggesting that the Met in the inactivation motif is not the only target.

We have examined the oxidation sensitivity of Na_V channel inactivation by replacing conserved Met residues in the IFM motif and other intracellular linkers of the rat Na_V1.4 channel and subjecting the expressed channels to oxidation. Mutation of Met1305 in the IFM motif in the D3–D4 linker drastically decreased oxidation sensitivity. Essentially the same effect was observed for two Met residues in the S4–S5 linker of domain 4 and also for a combination of the two mutants. The mutagenesis results and the kinetics of oxidation-induced modification of channel gating suggest that at least two Met residues are oxidized to impair inactivation. Because the mutation of other Met residues conserved among mammalian Na_V channel types had only minor effects, we postulate that the Met residues in the IFM motif and in its receptor are primarily responsible for the oxidation sensitivity of Na_V1.4 channel inactivation.

MATERIALS AND METHODS

Expression plasmids and mutagenesis

The α-subunit-encoding Na_V channel gene rNa_V1.4 (P15390; [21]) in the plasmid vector pcDNA3 was used as a background for mutagenesis. Site-specific mutagenesis was performed to replace methionine with leucine at positions 442, 1139, 1154, 1305, 1316, 1469, 1470. Mutant nomenclature is as follows: IFL: M1305L; IFM_LL: M1469L·M1470L; IFM_LM: M1469L; IFM_ML: M1470L; IFL_LL: M1305L·M1469L·M1470L; IFM_4L: M442L·M1139L·M1154L·M1316L; IFM_6L: IFM_LL combined with IFM_4L; IFL_6L: IFL combined with IFM_6L. As a control the following wild-type channels were used: rat Na_V1.2 (P04775; [22]), human Na_V1.7 (NP002968; [23]), and human Na_V1.5 (Q14524; [24]). Since the cardiac hNa_V1.5 channels harbour a cysteine residue in the pore region, it is sensitive to extracellular cysteine-modifying agents. We therefore constructed the mutant hNa_V1.5_C373Y to generate a pore region in domain-1 similar to that in Na_V1.4 channels. All channel types except for mutant IFL_6L expressed well, and typically cells with 1–10 nA of maximal inward current were included for analysis.

Cell culture

HEK 293 cells (CAMR, Porton Down, Salisbury, UK) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) mixed 1:1 with Ham’s F12 medium and supplemented with 10% fetal calf serum in a 5% CO₂ incubator. Cells were trypsinized, diluted with culture medium and grown in 35-mm dishes. Electrophysiological experiments were performed 1–5 days after plating. HEK 293 cells were transfected with the respective plasmids using the Rotifect® (Roth, Karlsruhe, Germany) transfection reagent following the vendor’s instructions.

Electrophysiological measurements

Whole-cell voltage-clamp experiments were performed as described previously [25]. Briefly, patch pipettes with resistances of 0.7–2.0 MΩ were used and the series resistance was compensated for >70% to minimize voltage errors. A patch-clamp amplifier EPC9 was operated by PatchMaster software (both HEKA Elektronik, Lambrecht, Germany). Holding potential was −120 mV. Leak and capacitive currents were corrected with a p/n method with a leak holding voltage of −120 mV. Currents were low-pass filtered at 5 kHz and sampled at a rate of 25 kHz. All experiments were performed at 19–21°C.

The patch pipettes contained (mM): 35 NaCl; 105 CsF; 10 EGTA; 10 HEPES (pH 7.4 with CsOH). The bath solution contained (mM): 150 NaCl; 2 KCl, 1.5 CaCl₂; 1 MgCl₂; 10 HEPES (pH 7.4 with NaOH).

Chloramine-T-induced oxidation

Chloramine-T (ChT) was diluted in the respective bath solution immediately before application. It was applied by total exchange of the bath solution within about 10 s.

Lucifer Yellow-induced oxidation

Cells were loaded with the fluorescent dye Lucifer Yellow (LY) (1 mM; Sigma) via the patch pipette. After recording control currents, cells were illuminated with 436-nm light from a PolyChrome-1 light source (XBO lamp, TILL Photonics) using a 40x dry objective.

Current-voltage relationships

From a holding potential of −120 mV cells were depolarized to −80 and +60 mV in steps of 10 mV for 40 ms every 3 s. The peak current-voltage relationships were fit according to a Hodgkin-Huxley formalism with m = 3 activation gates and a single-channel conductance according to the Goldman-Hodgkin-Katz equation.

$$I(V)=\mathrm{\Gamma }V\frac{1-e^{-(V-E_{\mathit{rev}})/25mV}}{1-e^{-V/25mV}}\frac{1}{{\left(1+e^{-(V-V_m)/k_m}\right)}^3}$$ (eq. 1)

V_m is the voltage of half-maximal gate activation and k_m the corresponding slope factor. Γ is the maximal conductance of all channels and E_rev the reversal potential.

Voltage dependence of fast inactivation

From a holding potential of −120 mV cells were conditioned for 500 ms at voltages ranging from −140 to 0 mV in steps of 5 mV. Subsequently, peak current was determined at −10 mV and normalised to a control peak current measured before conditioning. The repetition interval was 3 s. The normalised peak current plotted versus the conditioning voltage was described with a Boltzmann function:

$$\frac{I}{I_{\mathit{contr}}}(V)=\frac{1}{\left(1+e^{-(V-V_h)/k_h}\right)}$$ (eq. 2)

with the half-maximal inactivation voltage V_h and the corresponding slope factor k_h that indicates the voltage dependence of inactivation.

Time course of inactivation removal

The time course of oxidation-induced loss of inactivation was monitored by measuring the ratio of current after 10 ms and the peak current from pulses elicited to −10 mV at an interval of 5 s (r). The inactivation index, 1-r(t), was plotted as a function of time (t) and fit with the following function:

$$1-r(t)=1-r_0-(r_{\infty }-r_0){\left(1-e^{-(t-t_0)/\tau }\right)}^n$$ (eq. 3)

With the ratio before oxidation, r₀, the time of oxidation start, t₀, the time constant, τ, and an exponent, n. r_∞ is the ratio after infinite oxidation, which was set to 1 throughout.

Data analysis and statistics

Data were analysed with FitMaster (HEKA Elektronik) and IgorPro (WaveMetrics, Lake Oswego, OR, USA). Averaged data are presented as mean ± SEM (n = number of independent measurements). Groups of data were compared with a two-sided Student’s t-test or ANOVA followed by a post hoc Bonferroni test when appropriate.

RESULTS

Removal of fast inactivation by chloramine-T

Rat skeletal muscle sodium channels (Na_V1.4) were expressed in HEK 293 cells and Na⁺ currents were recorded in the whole-cell configuration in response to depolarizations to −10 mV applied every 5 s. The influence of various concentrations of extracellular chloramine-T (ChT) was assayed. Superposition of current traces measured before and 200 s after ChT application (Fig. 1a) shows that increasing ChT concentrations remove inactivation in a progressive manner. Similar removal of inactivation was observed at other test depolarizations (not shown). Channel activation was not markedly affected, but at high ChT (1 mM) concentration a peak current reduction could be observed after long waiting periods (not shown).

Fig. 1.

a–c Effect of ChT on the inactivation of Na_V1.4 channels in HEK 293 cells. a Current responses to depolarisations to −10 mV before (black) and 200 s after (grey) application of the indicated ChT concentration. b Superposition of inactivation index data from representative experiments in which the indicated concentration of ChT was applied at time zero. The superimposed curves are fits according to Eq. (3) with an exponent set to 2. The vertical grey bar indicates 200 s, i.e. the time from which the current traces in a were selected. c The time constants determined from fits as in panel b were transformed to rate constants (k = 1/τ) and plotted as a function of the ChT concentration. The error bars indicate SEM values (n = 4–11). The straight line is the result of a linear fit with a slope of 11.7 ± 0.5 (sM)⁻¹.

The inactivation index (see Materials and Methods), an operational descriptor proportional to the extent of inactivation, was plotted as a function of time (Fig. 1b). Increasing concentrations of ChT resulted in a loss of inactivation as indicated by a marked decrease in the inactivation index being about half-maximal after 100 s when 1 mM ChT was applied. The time course of inactivation removal could not be adequately described with a single exponential because the onset of the effect showed a noticeable degree of sigmoidicity. Therefore, as an operational data descriptor, we chose to fit the results with Eq. (3) assuming an exponent of n = 2. This approach resulted in reasonable data descriptions (see Fig. 1b), but it should be noted that deviations from an exponent of two would improve the fit considerably in some cases (see below).

The rate of ChT-induced loss of inactivation was estimated from the time constants of data fits as shown in Fig. 1b and is plotted in Fig. 1c as a function of ChT concentration. The rate roughly depends on the ChT concentration in a linear manner indicating that the approach to fit the time course with n = 2 extracted a rate constant describing the oxidation of a target in a first-order reaction.

As shown in Fig. 2a, progressive loss of inactivation induced by 200 μM ChT did not continue when ChT was washed away with external solutions containing the reducing agent dithiothreitol (DTT; 1 mM). DTT is expected to inactivate any residual ChT in the bath chamber and should restore thiol-modifications at cysteine residues. Even prolonged exposure to DTT did not restore inactivation (verified in each of the four independent experiments for the wild type and mutant M1316L); neither the time course of current inactivation nor the current magnitude changed upon DTT wash after ChT application (Fig. 2b). This result suggests that ChT either did not alter the redox state of cysteine residues or cysteine modification did not result in noticeable changes in channel function.

Fig. 2.

a, b Removal of inactivation is irreversible. a Inactivation index as a function of time; application of 200 μM ChT and wash with 1 mM DTT is indicated. b Current responses to depolarisations to −10 mV at the times during the experiment indicated by the white data points in a.

To ensure that the inactivation removal effect of ChT is caused by its ability to oxidize proteins, we used p-toluenesulfonamide (p-TSA). p-TSA is similar to ChT, but has an amide moiety instead of the N-NaCl group in ChT, and thus is incapable of generating reactive species. When applied to Na_V1.4 channels at a concentration of 2 mM for 1,000 s no alteration of channel inactivation was observed (n = 3; data not shown).

Effect of chloramine-T on various Na_V channel types

The time course of inactivation was also observed to be sensitive to oxidation in other Na_V channel types (e.g., [19,20,26]). To compare the sensitivity quantitatively, we expressed rNa_V1.2, hNa_V1.5, and hNa_V1.7 channels in HEK 293 cells and subjected them to ChT. Application of 200 μM extracellular ChT potently and rapidly blocked wild-type hNa_V1.5 channels (more than 50% in 30 s), probably because of a cysteine residue in the tetrodotoxin binding site in the pore loop of domain-1. Therefore we constructed and analysed mutant hNa_V1.5_C373Y in these experiments. This mutant has inactivation properties indistinguishable from those of the wild type, but the peak inward current is less sensitive to oxidation caused by ChT (only about 20% rapid current reduction upon application of 200 μM ChT). The inactivation of all channel types examined was similarly sensitive to ChT (Fig. 3).

Fig. 3.

a, b Removal of inactivation in Na_V isoforms rNa_V1.2, rNa_V1.4, hNa_V1.5_C373Y, and hNa_V1.7. a Superimposed current traces for depolarization from −120 mV to −10 mV before (black) and 500 s after application of 200 μM ChT (grey) for the indicated channel types. b Statistics on inactivation index after 500 s.

Influence of conserved methionine residues in the core domain of Na_V1.4 channels

Given the lack of reversibility under DTT application and the previously shown preference of ChT to oxidize Met residues [6,27], the loss of Na_V channel inactivation is probably mediated by oxidation of one or more methionines in the Na_V1.4 protein. A previous report has indeed suggested that Met oxidation plays a role in oxidation-induced removal of inactivation of Na_V channels in toad skeletal muscle fibres [19].

There is a conserved Met residue in the inactivation domain (IFM motif) between domain-3 and domain-4 (Fig. 4a). Thus we generated a mutant of Na_V1.4 in which this Met was replaced with leucine (M1305L, generating an IFL motif) and examined the sensitivity to ChT (Fig. 4b, top). As indicated by Wang and Wang [20], elimination of the Met from the IFM-motif fails to completely remove the oxidation sensitivity of the channel inactivation. However, the time course of ChT-induced loss of inactivation is much slower than in the wild type. Owing to this slow time course with 0.2 mM ChT, we could not accurately conclude if the slowing of inactivation reaches a steady state within a typical lifetime of a recording situation (30 min). However, treatment with ChT at 1 mM ChT resulted in complete loss of inactivation. Therefore, for kinetic analysis we assumed that ultimately all channels lose inactivation. Superposition of current traces from several cells recorded 300 s after start of ChT application (Fig. 4b, bottom) clearly shows that the IFL-mutant is less sensitive than the wild type. In Fig. 4c, the inactivation index after 500 s ChT (0.2 and 1.0 mM) application is presented. For the wild type this is 0.474 ± 0.035 (n = 10) and for mutant IFL 0.727 ± 0.017 (n = 5; P = 0.007) in 0.2 mM ChT; for 1.0 mM ChT the numbers are 0.019±0.007 (n = 8) and 0.449±0.050 (n = 4; P < 0.001). The finding that mutation of the Met residue in the IFM-motif alters but does not eliminate the oxidation sensitivity of the inactivation process suggests that other Met residues are involved.

Fig. 4.

a–c Influence of methionine mutations on the effect of ChT. a Schematic diagram illustrating the topology of a Na_V channel α-subunit. Conserved Met residues in the core domain excluding the N- and C-terminal regions are indicated as circles. The numbers refer to the positions in rNa_V1.4. b Examples of ChT-induced (200 μM) loss of inactivation (grey) with superimposed fits (black) according to Eq. (3) for the wild type and the indicated mutants. Bottom:: Superposition of current traces obtained from different cells exposed to 200 μM ChT for 300 s. c Mean inactivation index obtained 500 s after application of 0.2 mM (white) or 1.0 mM ChT (grey). Error bars are SEM values, n = 4–12.

The rat Na_V1.4 channel protein in total contains 66 Met residues making it impractical mutating all of them individually and in combination with the one in the inactivation domain. Since oxidation-induced loss of inactivation was also observed in other Na_V channel types (Fig. 3), one can speculate that the relevant methionines might be conserved. In addition, if such methionines were subject to cellular regulation such as reduction by methionine sulfoxide reductases, they are expected to be accessible from the cytosolic side. A multiple sequence alignment of all nine mammalian Na_V channels revealed that there are seven conserved Met residues in the core domain excluding the N- and C-terminal domains that face the cytosol (Fig. 4a). Most noticeably, there is a double-Met motif in the putative receptor for the inactivation domain in the S4–S5 linker of domain-4 (M1469, M1470) and two methionines in the corresponding linker of domain-3 (M1138, M1154). The remaining two methionines are in S6 of domain-1 (M442) and in the inactivation linker connecting domain-3 and domain-4 (1316). The documented relevance of the residues M1469 and M1470 for rapid inactivation [28] prompted us to generate a double mutant M1469L·M1470L, both in the background of the wild type (IFM) and in the background of the IFL mutant (termed IFM_LL and IFL_LL, respectively).

Both mutants, when exposed to 200 μM ChT, retained oxidation sensitivity, but the time course of inactivation removal was noticeably shower than in the wild-type channel (Fig. 4b). Using the inactivation index after 500 s of 0.2 and 1.0 mM ChT application as a measure (Fig. 4c), mutants IFM_LL (0.779 ± 0.015, n = 6; 0.394 ± 0.055, n = 5) and IFL_LL (0.863 ± 0.010, n = 6; 0.644 ± 0.076, n = 5) were less sensitive than the wild type (P < 0.05; P < 0.001), also shown in the superpositions of individual current traces 300 s after ChT application (Fig. b). In constructs already lacking the methionine(s) in the linker (IFL) or receptor (LL), removal of the other respective Met significantly slowed down the effect of ChT even further: IFL vs. IFL_LL, P < 0.001; IFM_LL vs. IFL_LL, P < 0.01. Thus, the Met in the IFM-motif as well as the Met diad in the putative receptor contribute to the oxidation sensitivity of Na_V channels in a somewhat additive manner. Individual mutations of methionines in the putative receptor revealed that M1470 is much more important for the channel’s sensitivity than M1469 (Fig. 4c): Inactivation index after 500 s in 1 mM ChT was 0.058 ± 0.025 (n = 6) for IFM_LM and 0.364 ± 0.044 (n = 5) for IFM_ML (P > 0.001). Nevertheless, even if all these methionines are replaced with leucines, the channels still lose inactivation suggesting the involvement of additional residues.

We therefore introduced further mutations altering M442, M1130, M1154, and M1316 to leucines into the mutants IFM_LL and IFL_LL, termed IFM_6L and IFL_6L, respectively. Unfortunately, the mutant IFL_6L gave rise to only very small Na⁺ current strongly compromising an accurate determination of its sensitivity towards ChT. Mutant IFM_6L expressed well, but exhibited a strongly left-shifted voltage dependence of steady-state inactivation; the half-maximal inactivation voltage (V_h) was −72.9 ± 0.7 mV (n = 22) for the wild type and −103.1 ± 1.5 mV (n = 6) for the mutant. As a consequence, even small left-shifts in the steady-state inactivation induced by ChT resulted in a substantial reduction of peak current even if the holding voltage was lowered from −120 mV to −140 mV. The determined loss of inactivation is, therefore, an overestimation, as properly inactivating channels will be selectively eliminated from the statistics owing to a shift in the steady-state inactivation. Nevertheless, mutant IFM_6L was significantly less sensitive to ChT than the wild type and mutants IFL and IFM_LL (inactivation index after 500 s: 0.904 ± 0.014, n = 6; 0.394 ± 0.040, n = 9 P < 0.001). The comparison of IFM_LL and IFM_6L shows that the additional replacement of the four methionines significantly diminishes the sensitivity of the channel further. This is corroborated by a small but significant decrease of sensitivity in mutant IFM_4L compared with the wild type (P = 0.002; Fig. 4c)

Time course of ChT-induced loss of inactivation

In the experiments shown in Fig. 4, the time course of the loss of inactivation mediated by application of 200 μM ChT was described with Eq. (3), allowing the exponent n to adjust, i.e. providing a variable sigmoidicity. This exponent was then plotted versus the respective time constant, τ, in Fig. 5a for the wild type and several mutants. Two trends are clearly observed: (1) There is a strong negative correlation between n and τ. (2) While the wild-type data require an n between 2 and 3, n approaches unity as the mutants lose their sensitivity towards ChT such that the sigmoidicity in the modification kinetics is diminished. The correlation between n and τ is consistent with the idea that the loss of inactivation by ChT is mediated by oxidative modification of multiple Met residues in the wild-type channel. As the target Met residues are removed, the exponent n approaches unity but the modification kinetics slows.

Fig. 5.

a, b Sigmoidicity of ChT-induced loss of inactivation. a Results of the fits according to Eq. (3) to data as shown in Figure 4b are plotted with their individual error estimates: exponent, n, indicating the degree of sigmoidicity, versus the time constant, τ. b Time course of the inactivation index of Na_V1.4 channels upon repeated application of 500 μM ChT. Between the ChT applications the cells were bathed in 1 mM DTT-containing solution. The continuous curve is a data fit assuming that the loss of inactivation proceeds under the second ChT application with the same kinetics as at the end of the first application. Exponent n = 2.01; time constant τ = 205 s.

The idea that multiple target residues account for the sigmoidal time course reflected in the greater-than-unity exponent value in the wild-type channel suggests that pretreatment of the channel with ChT should arrest them in a certain state. Loss of inactivation should resume without sigmoidicity after long interruptions of ChT application. In Fig. 5b, an experiment is shown illustrating that this is indeed the case. ChT was applied to wild-type channels and inactivation loss starts with an exponent of 2. Then ChT was washed away with DTT-solutions to stop the reaction immediately. After about 200 s, ChT was applied again and the loss of inactivation proceeded without any noticeable lag or sigmoidicity. This result also rules out that the sigmoidicity arises from slow accumulation of ChT inside the cell.

Alternative oxidation of Na_V1.4 channels by irradiated Lucifer Yellow

To test whether the effect of oxidation on Na_V channel inactivation is specific for ChT, we also applied H₂O₂ to HEK 293 cells expressing wild-type channels. As previously shown by Wang & Wang [20], H₂O₂ also removed inactivation (data not shown). Another means of generating reactive oxygen species in living cells is to load cells with the dye Lucifer Yellow (LY) via the patch pipette and to illuminate it with blue light (436 nm). This method was shown to be effective in removing inactivation of Na_V channels in mouse hippocampal neurons [26]. It has the great advantage that no mechanical agitation by changing of external bath solutions is required. Light-exposed LY (1 mM) effectively removed inactivation of the wild-type Na_V1.4 channel (Fig. 6a, b). The M-to-L mutants were much less sensitive than the wild type. The inactivation index after 500 s light exposure was 0.570 ± 0.076 (n = 7) for the wild type, 0.951 ± 0.009 (n = 6) for mutant IFL, and 0.971 ± 0.005 (n = 6) for mutant IFM_LL (P < 0.01). Similar to what was found for ChT, removal of methionines in the IFM domain and in the receptor in domain-4 strongly reduced the oxidation sensitivity of the channel. For LY we did not observe an additional effect of the extra methionines eliminated in the IFM_6L mutation because the protection obtained with mutation IFM_LL was almost complete already.

Fig. 6.

a–c Removal of inactivation with light-exposed Lucifer Yellow (LY). a Patch pipettes contained 1 mM LY and light (436 nm) was turned on at time zero. The left panels show examples of LY-induced loss of inactivation (grey) with superimposed fits (black) according to Eq. (3) for the wild type and the indicated mutants. Right: Mean inactivation index obtained 500 s after start of illumination. Errors are SEM values, n values are indicated in parentheses. b Superposition of current traces obtained from different cells exposed to LY and light for 300 s. c Results of the fits according to Eq. (3) to data as shown in panel a are plotted with their individual error estimates: exponent, n, indicating the degree of sigmoidicity, versus the time constant, τ.

The time course of LY-induced loss of inactivation of the wild type and the mutants was also described with Eq. (3). Similar to the experiments with ChT (Fig. 5a) we found a strong negative correlation between the estimated exponent (n) and the time constant (τ) (Fig. 6c). In addition, this analysis shows that the M-to-L mutations have a stronger protective effect when modification is mediated by LY irradiation as compared to ChT application: The time constant of wild-type modification was 388±37 s for ChT and 415±91 s for LY; mutation IFL slowed down the modification rate by a factor of 2.5±0.3 and 8.5±3.3, mutant IFM_LL by a factor of 2.1±0.3 and 9.3±4.7 for ChT and LY, respectively.

DISCUSSION

Regulation of proteins by oxidation is a common cellular mechanism, relevant to various physiological and pathophysiological phenomena and the process of aging (e.g., [3,29,30]). Therefore, the detailed investigation of molecular mechanisms involved in oxidative protein modification is of general interest. However, investigation of oxidative protein modification is not always straightforward, in part because of limited amino-acid specificity of the oxidizing agents. In case of thiol modifications of cysteine moieties, specific protocols are available, but oxidation of methionines requires harsher and potentially less specific treatments. Thus, unequivocal molecular characterization of oxidative protein modification requires the identification of target residues to exclude unspecific oxidation events.

In this study we investigated the sensitivity of voltage-gated sodium channels towards Met-directed oxidation caused by two independent oxidative treatments and identified multiple target Met-residues by mutagenesis. We expressed various Na_V channel types in HEK 293 cells and either applied the mild oxidant ChT or we irradiated LY, which was loaded into the cells via the patch pipettes. In both cases oxidation impaired the fast inactivation of Na_V channels in a time- and dose-dependent manner. This effect was almost identical in four different Na_V channel isoforms (Na_V1.2 (brain), Na_V1.4 (skeletal muscle), Na_V1.5_C373Y (heart), and Na_V1.7 (peripheral nervous system)). For the heart channel we used the single-point mutant C373Y in order to avoid direct oxidation of the tetrodotoxin binding site in the pore.

The reducing agent DTT failed to restore inactivation following treatment with ChT or LY-irradiation, suggesting that oxidation of Met residues might be involved in the removal of fast inactivation of the channel. Since all Na_V channel isoforms tested showed similar oxidation sensitivity, we reasoned that the relevant Met residues might be conserved. The most obvious Met residue (Met1305) involved in channel inactivation resides in the D3–D4 inactivation linker and is part of the “IFM motif”. When mutated to leucine, channel modification by ChT and irradiated LY was considerably slower, suggesting that Met1305 in the IFM motif is a potential target of oxidation.

This result is compatible with a previous report on the oxidation sensitivity of Na_V channel inactivation in toad skeletal muscle fibres, in which an involvement of Met residues was hypothesized [19]. Eaholtz and colleagues [31] provided more direct evidence by studying the potency of small peptides corresponding to the amino acid sequence of this inactivation domain, to “inactivate” or block Na_V channels. When in such peptides Ile-Phe-Met was replaced with Ile-Phe-MetO, the inactivating potency of the peptides vanished, suggesting that a Met-oxidized inactivation linker does not function properly. However, a report by Wang and Wang [20] suggests that the Met in the inactivation domain is not responsible for the loss of inactivation induced by either ultraviolet light or H₂O₂ as both interventions removed inactivation similarly in the Na_V1.4 wild-type channels and in a mutant in which the inactivation domain contained Ile-Phe-Ile. We saw that IFL channels retain some degree of oxidation sensitivity, but the time course of inactivation removal in the mutant was much slower than in the wild type. One likely explanation for this discrepancy could be that application of ChT or irradiation of LY more specifically attacks Met residues as compared to channel exposure to UV light or H₂O₂, which may more readily modify other amino acids.

Further mutagenesis revealed that the methionines in the receptor of the inactivation lid, Met1469/1470 in the S4–S5 linker of domain 4, also contribute to the channels’ oxidation sensitivity with M1470 playing a particularly important role. Combining leucine replacements at Met1305 and Met1469/1470 resulted in channels that were even less sensitive to oxidation. These results clearly show that inactivation of Na_V channels is sensitive to oxidative events. Loss of fast inactivation appears to be a multi-step process involving the oxidation of the IFM motif as well as its receptor site in domain 4. This was demonstrated by analyzing the time course of inactivation removal upon oxidation. While the wild-type channels showed a sigmoidicity with an exponent of two or greater, the mutated channels exhibited an exponent close to unity. The molecular interpretation could be that both, the inactivation lid and its receptor must be oxidized to effectively impair inactivation, essentially acting as a coincidence detector. When one target is removed by an M-to-L mutation, a single oxidation event is much less likely to occur such that only minor effects on channel inactivation are happening. This was particularly well observed when modification was elicited with irradiated LY in the patch pipette.

Additional conserved Met residues are present in intracellular regions of the channel protein (Fig. 4a). Most of them are located at the interface between membrane and cytosol to form a ring or cluster of residues at the intracellular entry of the channel. One could, therefore, speculate that these methionines may act as radical scavengers. If this was true, a channel with an intact IFM motif but with all other methionines absent might be more sensitive to oxidative stress than a channel with all native methionines in place. This scenario was tested with mutant IFM_6L, which, however, was not more oxidation sensitive than mutant IFM_LL. Thus, our experiments do not provide direct evidence for a scavenging mechanism inside Na_V channel proteins, but we cannot completely rule out this possibility for milder long-term oxidative stress situations.

Several examples of oxidation by ChT having a marked impact on ion channel function have been reported. Shaker potassium channels with prominent C-type inactivation [32] and hERG1 potassium channels [33] become non-functional when exposed to ChT. Calcium-activated potassium channels of the BK-type exhibit an increased activity on treatment with ChT [27] and this oxidative regulation requires a triad of Met residues in the C-terminal domain [34]. In Shaker C/B channels, treatment with ChT disrupts rapid N-type inactivation by a selective oxidation of a Met residue in the N-terminal inactivation ball domain [6,7]. The loss of N-type inactivation in Shaker C/B by ChT somewhat resembles the oxidative regulation of fast inactivation in Na_V channels reported here. However, the mechanism of the oxidative regulation in Na_V channels is more complex because our mutagenesis results show that multiple Met residues in the IFM inactivation motif and its receptor site are involved. This conclusion is further strengthened by the presence of sigmoidicity in the onset of loss of inactivation.

Oxidation of Met residues by ChT leads to formation of methionine sulfoxide and such posttranslational modification should, in principle, be reversible in the presence of methionine sulfoxide reductases (MSRs). Su et al. [33] report that the hERG1 channel is less sensitive to extracellularly applied ChT when MSRA is present in the patch pipette. We failed to observe such a protecting or repairing effect of MSRA on the inactivation of Na_V channels (data not shown). The reasons for that are not clear, but most likely a limited accessibility of the oxidized Met residues to the reducing enzyme or the relatively low catalytic turn-over rate of MSRA plays a role.

The importance of proper Na_V channel inactivation for neuronal and muscle functions is manifested by several inherited diseases (e.g. LQT-3 syndrome and myotonia; for an overview see [35]) in which the pathomechanism is postulated to involve an incomplete Na_V channel inactivation. Hence, under oxidative stress situations, incomplete Na_V channel inactivation may result in dramatic changes of electrical activity. A prominent example is cardiac arrhythmia occurring as a consequence of an ischemia/reperfusion insult; oxidation of cardiac Na_V channels (e.g. by application of H₂O₂) results in an increase in slowly inactivating, “late” sodium currents, which may cause fatal prolongations of cardiac action potentials [11,12]. Since the critical Met residues identified for Na_V1.4 channels are conserved in cardiac Na_V1.5 channels, similar molecular mechanisms of oxidation-induced loss of Na_V channel inactivation may also apply to the electrical properties of cardiac tissue. In fact, cardiac arrhythmia is part of the ischemia/reperfusion complications [36–38] and an impaired inactivation of cardiac Na_V channels would contribute an arrhythmogenic potential. A possible intervention could be a specific blockade of oxidized, non-inactivating channels as demonstrated for the beneficial effects of ranolazine for the postischemic heart [39].

Considering the potential pathophysiological consequences implicated in a loss of Na_V channel inactivation, it is surprising that oxidation-sensitive residues are conserved in the inactivation machinery although alternative residues (e.g., leucines) would produce Na_V channels with similarly rapid and complete inactivation. It thus appears as if Na_V channels carry an unnecessary “risk” of becoming oxidatively damaged. One speculation is that oxidation-regulated inactivation may offer an evolutionary advantage, allowing organisms to regulate firing frequency in neurons or contraction in cardiac tissue when cells are exposed to oxidative stress. The arrangement using multiple Met residues thereby confers a finely graded oxidative modulation of Na_V channels and allows the organisms to adapt to a variety of oxidative stress conditions. In this respect it is interesting to note that Na_V channels of insects (e.g. the Para channel of the fruit fly Drosophila melanogaster) harbour an “MFM” inactivation motif, which is expected to be even more sensitive to oxidation than their mammalian relatives. In addition, at the putative receptor site for the inactivation domain Para channels harbor an “AM” rather than an “MM” motif, i.e. residue M1470 shown to be important for the oxidation sensitivity of the channels is conserved. Although the physiological relevance still needs to be investigated, Na_V channels are prone of being oxidatively damaged if the oxidative stress exceeds a critical level, a phenomenon that may be relevant in various pathophysiological conditions and during the process of aging.