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. 1980 Mar;39(3):535–540. doi: 10.1128/aem.39.3.535-540.1980

Metabolism of Radiolabeled β-Guaiacyl Ether-Linked Lignin Dimeric Compounds by Phanerochaete chrysosporium

David A Weinstein 1,†, Kanit Krisnangkura 1,‡, Mary B Mayfield 1, Michael H Gold 1
PMCID: PMC291373  PMID: 16345527

Abstract

Phanerochaete chrysosporium metabolized the radiolabeled lignin model compounds [γ-14C]guaiacylglycerol-β-guaiacyl ether and [4-methoxy-14C]veratrylglycerol-β-guaiacyl ether (VI) to 14CO2 in stationary and in shaking cultures. 14CO2 evolution was greater in stationary culture. 14CO2 evolution from [γ-14C]guaiacyl-glycerol-β-guaiacyl ether and [4-methoxy-14C]veratrylglycerol-β-guaiacyl ether in stationary cultures was two- to threefold greater when 100% O2 rather than air (21% O2) was the gas phase above the cultures. 14CO2 evolution from the metabolism of the substrates occurred only as the culture entered the stationary phase of growth. The presence of substrate levels of nitrogen in the medium suppressed 14CO2 evolution from both substrates in stationary cultures. [14C]veratryl alcohol and 4-ethoxy-3-methoxybenzyl alcohol were formed as products of the metabolism of VI and 4-ethoxy-3-methoxyphenylglycerol-β-guaiacyl ether, respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crawford R. L., Kirk T. K., Harkin J. M., McCoy E. Bacterial cleavage of an arylglycerol- -aryl ether bond. Appl Microbiol. 1973 Feb;25(2):322–324. doi: 10.1128/am.25.2.322-324.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fukuzumi T., Takatuka H., Minami K. Enzymic degradation of lignin. V. The effect of NADH on the enzymic cleavage of arylalkylether bond in veratrylglycerol-beta-guaiacylether as lignin model compound. Arch Biochem Biophys. 1969 Jan;129(1):396–403. doi: 10.1016/0003-9861(69)90191-x. [DOI] [PubMed] [Google Scholar]
  3. Gold M. H., Cheng T. M. Induction of colonial growth and replica plating of the white rot basidiomycete Phanaerochaete chrysosporium. Appl Environ Microbiol. 1978 Jun;35(6):1223–1225. doi: 10.1128/aem.35.6.1223-1225.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gold M. H., Mitzel D. L., Segel I. H. Regulation of nigeran accumulation by Aspergillus aculeatus. J Bacteriol. 1973 Feb;113(2):856–862. doi: 10.1128/jb.113.2.856-862.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Keyser P., Kirk T. K., Zeikus J. G. Ligninolytic enzyme system of Phanaerochaete chrysosporium: synthesized in the absence of lignin in response to nitrogen starvation. J Bacteriol. 1978 Sep;135(3):790–797. doi: 10.1128/jb.135.3.790-797.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kirk T. K., Connors W. J., Bleam R. D., Hackett W. F., Zeikus J. G. Preparation and microbial decomposition of synthetic [14C]ligins. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2515–2519. doi: 10.1073/pnas.72.7.2515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kirk T. K., Harkin J. M., Cowling E. B. Degradation of the lignin model compound syringylglycol-beta-guaiacyl ether by Polyporus versicolor and Stereum frustalatum. Biochim Biophys Acta. 1968 Aug 6;165(1):145–163. doi: 10.1016/0304-4165(68)90199-2. [DOI] [PubMed] [Google Scholar]
  8. Kirk T. K., Harkin J. M., Cowling E. B. Oxidation of guaiacyl- and veratryl-glycerol-beta-gualacyl ether by Polyporus versicolor and Stereum frustulatum. Biochim Biophys Acta. 1968 Aug 6;165(1):134–144. doi: 10.1016/0304-4165(68)90198-0. [DOI] [PubMed] [Google Scholar]
  9. RUSSELL J. D., HENDERSON M. E., FARMER V. C. Metabolism of lignin model compounds by Polystictus versicolor. Biochim Biophys Acta. 1961 Sep 30;52:565–570. doi: 10.1016/0006-3002(61)90415-2. [DOI] [PubMed] [Google Scholar]

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