Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1980 Mar;39(3):572–575. doi: 10.1128/aem.39.3.572-575.1980

Impairment of phagocytosis by heterophils from chickens during ochratoxicosis.

C F Chang, P B Hamilton
PMCID: PMC291380  PMID: 7387156

Abstract

The effect of graded concentrations of dietary ochratoxin A (0, O.5, 1.0, 2.0, 4.0, and 8.0 microgram/g of diet) on the in vitro phagocytic, locomotory, and bactericidal capacities of heterophils from broiler chickens was investigated. Both the percentage and the mean phagocytic activities were decreased significantly (P less than 0.05) at 4.0 and 8.0 microgram/g. Both directed and undirected locomotion of heterophila was impaired significantly at the same concentrations. A crossover experiment revealed that the reduced percentage of phagocytosis was associated with the heterophil itself and not with a serum factor such as complement. Heterophila from birds that consumed 4.0 microgram/g were not impaired in ability to kill engulfed bacteria.

Full text

PDF
574

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altman L. C., Kirchner H. Mononuclear leucocyte chemotaxis in the chicken. Definition of a phylogenetically specific lymphokine. Immunology. 1974 Feb;26(2):393–405. [PMC free article] [PubMed] [Google Scholar]
  2. Applegate K. L., Chipley J. R. Ochratoxins. Adv Appl Microbiol. 1973;16:97–109. doi: 10.1016/s0065-2164(08)70024-8. [DOI] [PubMed] [Google Scholar]
  3. COHN Z. A., MORSE S. I. Interactions between rabbit polymorphonuclear leucocytes and staphylococci. J Exp Med. 1959 Sep 1;110:419–443. doi: 10.1084/jem.110.3.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chang C. F., Hamilton P. B. Impaired phagocytosis by heterophils from chickens during aflatoxicosis. Toxicol Appl Pharmacol. 1979 May;48(3):459–466. doi: 10.1016/0041-008x(79)90429-0. [DOI] [PubMed] [Google Scholar]
  5. Chu F. S. Studies on ochratoxins. CRC Crit Rev Toxicol. 1974 Jan;2(4):499–524. doi: 10.3109/10408447309025706. [DOI] [PubMed] [Google Scholar]
  6. Clark R. A., Kimball H. R. Defective granulocyte chemotaxis in the Chediak-Higashi syndrome. J Clin Invest. 1971 Dec;50(12):2645–2652. doi: 10.1172/JCI106765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Giambrone J. J., Ewert D. L., Wyatt R. D., Eidson C. S. Effect of aflatoxin on the humoral and cell-mediated immune systems of the chicken. Am J Vet Res. 1978 Feb;39(2):305–308. [PubMed] [Google Scholar]
  8. Michael G. Y., Thaxton P., Hamilton P. B. Impairment of the reticuloendothelial system of chickens during aflatoxicosis. Poult Sci. 1973 May;52(3):1206–1207. doi: 10.3382/ps.0521206. [DOI] [PubMed] [Google Scholar]
  9. Noble P. B., Cutts J. H. Isolation of individual leukocyte types from peripheral blood. J Lab Clin Med. 1968 Sep;72(3):533–538. [PubMed] [Google Scholar]
  10. Quie P. G., White J. G., Holmes B., Good R. A. In vitro bactericidal capacity of human polymorphonuclear leukocytes: diminished activity in chronic granulomatous disease of childhood. J Clin Invest. 1967 Apr;46(4):668–679. doi: 10.1172/JCI105568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Richard J. L., Thurston J. R., Deyoe B. L., Booth G. D. Effect of ochratoxin and aflatoxin on serum proteins, complement activity, and antibody production to Brucella abortus in guinea pigs. Appl Microbiol. 1975 Jan;29(1):27–29. doi: 10.1128/am.29.1.27-29.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Richard J. L., Thurston J. R. Effect of aflatoxin on phagocytosis of Aspergillus fumigatus spores by rabbit alveolar macrophages. Appl Microbiol. 1975 Jul;30(1):44–47. doi: 10.1128/am.30.1.44-47.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Thaxton J. P., Tung H. T., Hamilton P. B. Immunosuppression in chickens by aflatoxin. Poult Sci. 1974 Mar;53(2):721–725. doi: 10.3382/ps.0530721. [DOI] [PubMed] [Google Scholar]
  14. Trenk H. L., Butz M. E., Chu F. S. Production of ochratoxins in different cereal products by Aspergillus ochraceus. Appl Microbiol. 1971 Jun;21(6):1032–1035. doi: 10.1128/am.21.6.1032-1035.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ward P. A., Lepow I. H., Newman L. J. Bacterial factors chemotactic for polymorphonuclear leukocytes. Am J Pathol. 1968 Apr;52(4):725–736. [PMC free article] [PubMed] [Google Scholar]
  16. van der Merwe K. J., Steyn P. S., Fourie L., Scott D. B., Theron J. J. Ochratoxin A, a toxic metabolite produced by Aspergillus ochraceus Wilh. Nature. 1965 Mar 13;205(976):1112–1113. doi: 10.1038/2051112a0. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES