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. 1980 Mar;39(3):630–634. doi: 10.1128/aem.39.3.630-634.1980

Cytoplasmic reserve polysaccharide of Selenomonas ruminantium.

R J Wallace
PMCID: PMC291388  PMID: 7387159

Abstract

Selenomonas ruminantium accumulated large quantities of intracellular polysaccharide when grown in simple defined medium in a chemostat, particularly at low dilution rate under NH3 limitation when the carbohydrate content of the cells was greater than 40% of the dry weight. This polysaccharide was used as a source of energy under conditions of energy starvation. Abundant, densely staining cytoplasmic granules were observed by electron microscopy in sections stained by the periodic acid-thiocarbohydrazide-osmium technique. The polysaccharide was extracted in 30% KOH followed by precipitation with 60% ethanol and was found to be a glucose homopolymer. Sepharose 4B gel filtration and iodine-complex spectroscopy showed that the polysaccharide was of the glycogen type with a molecular weight of 5 X 10(5) to greater than 20 X 10(5) and an average chain length of 12 glucose residues.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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